Golgi-like labeling of a single neuron recorded extracellularly.

Abstract:

:We describe a novel and powerful extracellular method for the staining of a single neuron identified by electrophysiological criteria. This single-unit technique involves the use of glass micro-electrodes (tip diameter: 1.5-2.5 microns) filled with a saline solution (NaCl; 0.5 M) containing 1.5% of biocytin or Neurobiotin. Once a neuron is recorded, isolated and identified, the tracer is delivered by anodal current pulses of a few nA. The cell must remain well isolated and alive during the ejection procedure to ensure optimal staining. Evidence is provided that the labeled neuron is actually the one that was recorded. Our simple method is reliable with a success rate exceeding 85%. The advantages and pitfalls are discussed. This single-unit labeling technique could further be combined with any other procedures ranging from biological to behavioral studies.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Pinault D

doi

10.1016/0304-3940(94)90332-8

subject

Has Abstract

pub_date

1994-04-11 00:00:00

pages

255-60

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(94)90332-8

journal_volume

170

pub_type

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