Abstract:
:In this study, we investigated the mechanistic role and prognostic significance of the long coding RNA (lncRNA) KCNQ1OT1 in colorectal cancer (CRC). KCNQ1OT1 levels were significantly higher in CRC tissues than adjacent normal colorectal tissues (n=79). High KCNQ1OT1 expression correlated with poorer prognosis in CRC patients. KCNQ1OT1-silenced CRC cells showed reduced proliferation, colony formation, extracellular acidification, and lactate and glucose secretion. This suggests KCNQ1OT1 promotes CRC cell proliferation by increasing aerobic glycolysis. RNA pull-down assays with biotinylated KCNQ1OT1 followed by mass spectrometry analysis showed that KCNQ1OT1 directly binds to hexokinase 2 (HK2). This was confirmed by RNA immunoprecipitation assays using anti-hexokinase 2 antibody. HK2 protein levels were reduced in KCNQ1OT1 knockdown CRC cells, but were restored by treatment with the proteasomal inhibitor MG132. KCNQ1OT1 knockdown CRC cells also showed higher ubiquitinated-HK2 levels, suggesting KCNQ1OT1 enhances aerobic glycolysis by stabilizing HK2. HK2 overexpression in KCNQ1OT1 knockdown CRC cells restored proliferation and aerobic glycolysis. KCNQ1OT1 levels correlated positively with HK2 expression and prognosis in CRC patients. These findings show that KCNQ1OT1 promotes colorectal carcinogenesis by increasing aerobic glycolysis through HK2.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Chen C,Wei M,Wang C,Sun D,Liu P,Zhong X,Yu Wdoi
10.18632/aging.103334subject
Has Abstractpub_date
2020-06-21 00:00:00pages
11685-11697issue
12issn
1945-4589pii
103334journal_volume
12pub_type
杂志文章相关文献
Aging-US文献大全abstract::Telomeres are short segments in chromosome ends, the length of which is reduced during cell lifecycles. We examined the association of mean leukocyte telomere length (LTL) and short telomere proportion (STP) with hemodynamic variables in normotensive and never-treated hypertensive volunteers (n=566, 19-72 years). STP ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103459
更新日期:2020-06-23 00:00:00
abstract::Bladder cancer (BCa) is a heterogeneous disease with various tumorigenic mechanisms and clinical behaviors. The current tumor-node-metastasis (TNM) staging system is inadequate to predict overall survival (OS) in BCa patients. We developed a BCa-specific, long-non-coding-RNA (lncRNA)-based nomogram to improve survival...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102782
更新日期:2020-02-12 00:00:00
abstract::One important process in liver cancer growth and progression is angiogenesis. Vascular endothelial growth factor (VEGF) has the significant role in liver cancer angiogenesis. sFlt1 (soluble Fms-like tyrosine kinase-1) is the promising inhibitor of VEGF and can be used as the new method of inhibiting angiogenesis. MSCs...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101146
更新日期:2016-12-28 00:00:00
abstract::Inflammation of the vascular microenvironment modulates distinct types of vascular cells, and plays important roles in promoting atherosclerosis, stenosis/restenosis, and vascular-related diseases. Nik-related kinase (Nrk), a member of the Ste20-type kinase family, has been reported to be selectively expressed in embr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103104
更新日期:2020-04-24 00:00:00
abstract::Amine neurotransmitters, such as dopamine, serotonin, and noradrenaline, play important roles in the modulation of behaviors and metabolism of animals. InC. elegans, it has been shown that serotonin and octopamine, an invertebrate equivalent of noradrenaline, also regulate lifespan through a mechanism related to food ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100097
更新日期:2009-10-21 00:00:00
abstract::Chondrocyte apoptosis and extracellular matrix (ECM) degeneration have been implicated in the pathogenesis of osteoarthritis (OA). Based on previously reported microarray analysis, HRAS (Harvey rat sarcoma viral oncogene homolog), a member of the RAS protein family, was chosen as a potential regulator of OA chondrocyt...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102801
更新日期:2020-02-12 00:00:00
abstract::Aging and age-related diseases occur in almost all organisms. Recently, it was discovered that the inhibition of target of rapamycin complex 1 (TORC1), a conserved complex that mediates nutrient status and cell metabolism, can extend an individual's lifespan and inhibit age-related diseases in many model organisms. Ho...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102428
更新日期:2019-11-11 00:00:00
abstract::The incidence of subarachnoid hemorrhage (SAH) and hazard ratio of death increase with age. Overactivation of microglia contributes to brain damage. This study aimed to investigate the effects of A3 adenosine receptors (A3R) activation on neurofunction and microglial phenotype polarization in the context of SAH in age...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202178
更新日期:2020-11-30 00:00:00
abstract::The Editorial Board of Aging reviews research papers published in 2009, which they believe have or will have significant impact on aging research. Among many others, the topics include genes that accelerate aging or in contrast promote longevity in model organisms, DNA damage responses and telomeres, molecular mechani...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100132
更新日期:2010-03-01 00:00:00
abstract::This study compares the longitudinal histological characteristics of proximal humeral implants with different spatial structures in rabbits. Thirty skeletally-mature male rabbits were divided into a trabecular structure group and regular hexahedron structure group according to the different spatial structures of a bio...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202391
更新日期:2021-01-20 00:00:00
abstract::The epidermis is continuously renewed by stem cell proliferation and differentiation. Basal keratinocytes append the dermal-epidermal junction, a cell surface-associated, extracellular matrix that provides structural support and influences their behaviour. It consists of laminins, type IV collagen, nidogens, and perle...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100928
更新日期:2016-04-01 00:00:00
abstract::DNA damaging agents and radiation, cytotoxins and anti-cancer drugs, telomere erosion and cytokines, culture shock and mitogenic stimuli, oncogenes and tumor suppressors can induce both cell cycle arrest and cellular senescence. Due to this semi-coincidence, senescence is confused with cell cycle arrest, or even more ...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100281
更新日期:2011-02-01 00:00:00
abstract::Background: Aging has often been linked to age-related vascular disorders. The elucidation of the putative genes and pathways underlying vascular aging likely provides useful insights into vascular diseases at advanced ages. Transcriptional regulatory network analysis is the key to describing genetic interactions betw...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102275
更新日期:2019-09-12 00:00:00
abstract::Despite the accumulating evidence from in vitro and animal experiments supporting the role of kynurenine (a tryptophan metabolite) in a number of degenerative age-related changes, the relationship between kynurenine and frailty in older adults is not well understood. We collected blood samples from 73 participants who...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104179
更新日期:2020-11-13 00:00:00
abstract::Impaired quality of life (QOL) is common in hepatocellular carcinoma (HCC) patients. In this study, we used a large hospital-based multiethnic HCC patient cohort to systematically identify factors associated with QOL and investigate the prognostic value of QOL.The Short Form-12 questionnaire was used to assess QOL. Th...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102889
更新日期:2020-03-07 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by glucose metabolic disorders, and gluconeogenesis inhibiting is a promisingly therapeutic strategy for T2DM. Glucocorticoid receptor (GR) is tightly implicated in the regulation of gluconeogenesis, although the underlying mechanism remains ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202275
更新日期:2020-12-09 00:00:00
abstract::Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 8...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103465
更新日期:2020-08-05 00:00:00
abstract::Since the initial description of apoptosis, a number of different forms of cell death have been described. In this review we will focus on classic caspase-dependent apoptosis and its variations that contribute to diseases. Over fifty years of research have clarified molecular mechanisms involved in apoptotic signaling...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100459
更新日期:2012-05-01 00:00:00
abstract::Proliferating cell nuclear antigen binding factor (encoded by KIAA0101/PCLAF) regulates DNA synthesis and cell cycle progression; however, whether the level of KIAA0101 mRNA in lung adenocarcinoma is related to prognosis and tumor immune infiltration is unknown. In patients with lung adenocarcinoma, the differential e...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104144
更新日期:2020-11-20 00:00:00
abstract::The development and progression of hepatocellular carcinoma (HCC) is accompanied with persistent oxidative stress, but the molecular basis is not well defined. Superoxide dismutase 2 (SOD2) is an important mitochondrial antioxidant and a key aging factor. Here we investigated the expression and clinical significance o...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100967
更新日期:2016-06-01 00:00:00
abstract::To investigate the association of homocysteine concentration with intracranial atherosclerotic stenosis (ICAS), we assessed 933 acute ischemic stroke patients (346 with ICAS, 587 without ICAS) and 798 non-stroke controls (175 with ICAS, 623 without ICAS) with magnetic resonance angiography (MRA). Homocysteine concentr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102019
更新日期:2019-06-12 00:00:00
abstract::The tumor suppressor LKB1 has emerged as a critical regulator of cell polarity and energy‐metabolism. Studies in diverse model organisms continue to unravel the pathways downstream of LKB1; the emerging picture is that the outcomes of LKB1 signaling are mediated by a plethora of tissue‐specific and context‐dependent e...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100319
更新日期:2011-04-01 00:00:00
abstract::Traditionally, the p21 protein has been viewed as limiting cancer progression and promoting aging. In contrast, there are reports that p21 can enhance cancer survival and limit tissue damage, depending on the tissue of origin and type of stressor involved. Here, we provide evidence to support these latter two roles of...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103961
更新日期:2020-09-24 00:00:00
abstract::New strategies to block the immune evasion activity of programmed death ligand-1 (PD-L1) are urgently needed. When exploring the PD-L1-targeted effects of mechanistically diverse metabolism-targeting drugs, exposure to the dietary polyphenol resveratrol (RSV) revealed its differential capacity to generate a distinct P...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102646
更新日期:2020-01-04 00:00:00
abstract::Neural stem cell (NSC) transplantation has prevailed as a promising protective strategy for cardiac arrest (CA)-induced brain damage. Surprisingly, the poor survival of neuronal cells in severe hypoxic condition restricts the utilization of this cell-based therapy. Extracellular vesicles (EVs) transfer microRNAs (miRN...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103920
更新日期:2021-01-12 00:00:00
abstract::Cardiac remodeling and subsequent heart failure remain critical issues after myocardial infarction despite improved treatment and reperfusion strategies. Recently, complete cardiac regeneration has been demonstrated in fish and newborn mice following resection of the cardiac apex. However, it remained entirely unclear...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100526
更新日期:2012-12-01 00:00:00
abstract::Autophagy is involved in degenerative diseases such as osteoarthritis and disc degeneration. Although, tumor necrosis factor α-induced protein 3 (TNFAIP3) is well-known as a key regulator of inflammation and autophagy, it is still not clear whether TNFAIP3 regulates autophagy to protect from human disc cells degenerat...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104160
更新日期:2020-11-20 00:00:00
abstract::Circular RNAs (circRNAs), a novel class of endogenous long non-coding RNAs, have attracted considerable attention due to their closed continuous loop structure and potential clinical value. In this study, we investigated the function of circFASTKD1 in vascular endothelial cells. CircFASTKD1 bound directly to miR-106a ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202305
更新日期:2020-12-19 00:00:00
abstract:OBJECTIVE:The high mortality rate associated with acute respiratory distress syndrome (ARDS) is a major challenge for intensive care units. In the present study, we applied bioinformatics and animal models to identify core genes and potential corresponding pathways in ARDS. RESULTS:Using bioinformatics analysis, IL-1β...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102804
更新日期:2020-02-15 00:00:00
abstract::Excess salt (NaCl) intake is closely related to a variety of central nervous system (CNS) diseases characterized by increased cerebral microvascular permeability. However, the link between a high salt diet (HSD) and the breakdown of tight junctions (TJs) remains unclear. In the present study, we found that high salt d...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103348
更新日期:2020-06-22 00:00:00