Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene.


:A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10² DNA copies/μL, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.


Virol J


Virology journal


Song C,Zhu C,Zhang C,Cui S




Has Abstract


2010-12-02 00:00:00










  • Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China.

    abstract:BACKGROUND:Astroviruses (AstVs) have been reported to infect and cause gastroenteritis in most animal species. Human AstVs were regarded the causative agent of viral diarrhea in children. In dogs, little is known about the epidemiology and clinical significance of AstV infection. FINDINGS:In this study, we collected a...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Zhou H,Liu L,Li R,Qin Y,Fang Q,Balasubramaniam VR,Wang G,Wei Z,Ouyang K,Huang W,Chen Y

    更新日期:2017-08-17 00:00:00

  • Purification of infectious human herpesvirus 6A virions and association of host cell proteins.

    abstract:BACKGROUND:Viruses that are incorporating host cell proteins might trigger autoimmune diseases. It is therefore of interest to identify possible host proteins associated with viruses, especially for enveloped viruses that have been suggested to play a role in autoimmune diseases, like human herpesvirus 6A (HHV-6A) in m...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Hammarstedt M,Ahlqvist J,Jacobson S,Garoff H,Fogdell-Hahn A

    更新日期:2007-10-19 00:00:00

  • Tumor necrosis factor-α-induced protein 8-like 2 mRNA in peripheral blood mononuclear cells is associated with the disease progression of chronic hepatitis B virus infection.

    abstract:BACKGROUND:Tumor necrosis factor-alpha-induced protein 8-like 2 (TIPE2) is a novel target and molecule in the negative regulation of immune homeostasis. The present study aimed to investigate the dynamic expression of TIPE2 mRNA during the progression of chronic hepatitis B virus (HBV) infection. METHODS:A total of 19...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Liu Y,Jin J,Ji J,Gao XM,Fan YC

    更新日期:2019-10-28 00:00:00

  • Torquetenovirus detection in exosomes enriched vesicles circulating in human plasma samples.

    abstract:BACKGROUND:Torquetenovirus (TTV) belongs to Anelloviridae family, infects nearly all people indefinitely without causing overt disease establishing a fine and successful interaction with the host. Increasing evidence have shown some human viruses exploit extracellular vesicles thereby helping viral persistence in the h...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Martelli F,Macera L,Spezia PG,Medici C,Pistello M,Guasti D,Romagnoli P,Maggi F,Giannecchini S

    更新日期:2018-09-20 00:00:00

  • Bioinformatics analysis of rabbit haemorrhagic disease virus genome.

    abstract:BACKGROUND:Rabbit haemorrhagic disease virus (RHDV), as the pathogeny of Rabbit haemorrhagic disease, can cause a highly infectious and often fatal disease only affecting wild and domestic rabbits. Recent researches revealed that it, as one number of the Caliciviridae, has some specialties in its genome, its reproducti...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Tian XT,Li BY,Zhang L,Jiao WQ,Liu JX

    更新日期:2011-11-01 00:00:00

  • Infectious pancreatic necrosis virus (IPNV) from salmonid fish enters, but does not replicate in, mammalian cells.

    abstract:BACKGROUND:The aquatic birnavirus infectious pancreatic necrosis virus (IPNV) causes infectious pancreatic necrosis (IPN), a severe disease in farmed salmonid fish. IPNV has a very broad host range and infects many different species of fish as well as molluscs and crustaceans. Investigation of the host reservoir of a v...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Ørpetveit I,Küntziger T,Sindre H,Rimstad E,Dannevig BH

    更新日期:2012-10-05 00:00:00

  • Association of LIN28B polymorphisms with chronic hepatitis B virus infection.

    abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Han Q,Sang J,Fan X,Wang X,Zeng L,Zhang X,Zhang K,Li N,Lv Y,Liu Z

    更新日期:2020-06-22 00:00:00

  • Role of antenatal plasma cytomegalovirus DNA levels on pregnancy outcome and HIV-1 vertical transmission among mothers in the University of Zimbabwe birth cohort study (UZBCS).

    abstract:INTRODUCTION:Despite being a leading infectious cause of childhood disability globally, testing for cytomegalovirus (CMV) infections in pregnancy is generally not done in Sub-Sahara Africa (SSA), where breastfeeding practice is almost universal. Whilst CMV and human immunodeficiency virus (HIV) are both endemic in SSA,...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Duri K,Chimhuya S,Gomo E,Munjoma PT,Chandiwana P,Yindom LM,Mhandire K,Ziruma A,Mtapuri-Zinyowera S,Mazengera LR,Misselwitz B,Gumbo FZ,Jordi S,Rowland-Jones S,for (UZBCS) The U Z Birth Cohort Study Team.

    更新日期:2021-01-29 00:00:00

  • Full-length genome sequence analysis of enzootic nasal tumor virus isolated from goats in China.

    abstract:BACKGROUND:Enzootic nasal tumor virus (ENTV) is a betaretrovirus of sheep (ENTV-1) and goats (ENTV-2) associated with neoplastic transformation of epithelial cells of the ethmoid turbinate. Confirmation of the role of ENTV in the pathogenesis of enzootic nasal adenocarcinoma (ENA) has yet to be resolved due to the inab...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: He Y,Zhang Q,Wang J,Zhou M,Fu M,Xu X

    更新日期:2017-07-26 00:00:00

  • Systemic virus distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virus.

    abstract:BACKGROUND:Avian influenza virus (AIV) is classified into two pathotypes, low pathogenic (LP) and high pathogenic (HP), based on virulence in chickens.Differences in pathogenicity between HPAIV and LPAIV might eventually be related to specific characteristics of strains, tissue tropism and host responses. METHODS:To s...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Post J,Burt DW,Cornelissen JB,Broks V,van Zoelen D,Peeters B,Rebel JM

    更新日期:2012-03-06 00:00:00

  • Progressive multifocal leukoencephalopathy in a patient without apparent immunosuppression.

    abstract::An 80-year-old man with no history of an immune-compromising disorder was diagnosed with progressive multifocal leukoencephalopathy (PML). He presented with dysphagia and left-sided weakness; magnetic resonance imaging demonstrated marked signal abnormality in the subcortical white matter of the left frontal lobe and ...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Vaklavas C,Sotelo-Rafiq EP,Lovy J,Escobar MA,Tsimberidou AM

    更新日期:2010-09-28 00:00:00

  • Transmission of H7N9 influenza virus in mice by different infective routes.

    abstract:BACKGROUND:On 19 February 2013, the first patient infected with a novel influenza A H7N9 virus from an avian source showed symptoms of sickness. More than 349 laboratory-confirmed cases and 109 deaths have been reported in mainland China since then. Laboratory-confirmed, human-to-human H7N9 virus transmission has not b...

    journal_title:Virology journal

    pub_type: 历史文章,杂志文章


    authors: Bao L,Xu L,Zhu H,Deng W,Chen T,Lv Q,Li F,Yuan J,Xu Y,Huang L,Li Y,Liu J,Yao Y,Yu P,Chen H,Qin C

    更新日期:2014-11-03 00:00:00

  • Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012.

    abstract:BACKGROUND:Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. An outbreak of aseptic meningitis occurred from May to June, 2012, in Guangdong Province, China. In order to determine the etiolog...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Xiao H,Guan D,Chen R,Chen P,Monagin C,Li W,Su J,Ma C,Zhang W,Ke C

    更新日期:2013-08-23 00:00:00

  • Novel snake papillomavirus does not cluster with other non-mammalian papillomaviruses.

    abstract::Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals. We identified, cloned and sequenced PV DNA from pigmented papilloma-like lesions of a diamond python (Morelia spilota spilota). This represents the first ...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Lange CE,Favrot C,Ackermann M,Gull J,Vetsch E,Tobler K

    更新日期:2011-09-12 00:00:00

  • Single amino acid change in gp41 region of HIV-1 alters bystander apoptosis and CD4 decline in humanized mice.

    abstract:BACKGROUND:The mechanism by which HIV infection leads to a selective depletion of CD4 cells leading to immunodeficiency remains highly debated. Whether the loss of CD4 cells is a direct consequence of virus infection or bystander apoptosis of uninfected cells is also uncertain. RESULTS:We have addressed this issue in ...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Garg H,Joshi A,Ye C,Shankar P,Manjunath N

    更新日期:2011-01-21 00:00:00

  • Caveolin-1 influences human influenza A virus (H1N1) multiplication in cell culture.

    abstract:BACKGROUND:The threat of recurring influenza pandemics caused by new viral strains and the occurrence of escape mutants necessitate the search for potent therapeutic targets. The dependence of viruses on cellular factors provides a weak-spot in the viral multiplication strategy and a means to interfere with viral multi...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Sun L,Hemgård GV,Susanto SA,Wirth M

    更新日期:2010-05-26 00:00:00

  • In vitro antiviral activity of plant extracts from Asteraceae medicinal plants.

    abstract:BACKGROUND:Due to the high prevalence of viral infections having no specific treatment and the constant appearance of resistant viral strains, the development of novel antiviral agents is essential. The aim of this study was to evaluate the antiviral activity against bovine viral diarrhea virus, herpes simplex virus ty...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Visintini Jaime MF,Redko F,Muschietti LV,Campos RH,Martino VS,Cavallaro LV

    更新日期:2013-07-27 00:00:00

  • Rubella, herpes simplex virus type 2 and preeclampsia.

    abstract:BACKGROUND:Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE:The aim of the present study was to investigate the association between maternal seropositivity of rubella, He...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Alshareef SA,Eltom AM,Nasr AM,Hamdan HZ,Adam I

    更新日期:2017-07-26 00:00:00

  • A novel candidate HIV vaccine vector based on the replication deficient Capripoxvirus, Lumpy skin disease virus (LSDV).

    abstract:BACKGROUND:The Capripoxvirus, Lumpy skin disease virus (LSDV) has a restricted host-range and is being investigated as a novel HIV-1 vaccine vector. LSDV does not complete its replication cycle in non-ruminant hosts. METHODS:The safety of LSDV was tested at doses of 104 and 106 plaque forming units in two strains of i...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Shen YJ,Shephard E,Douglass N,Johnston N,Adams C,Williamson C,Williamson AL

    更新日期:2011-05-30 00:00:00

  • Torque teno virus dynamics during the first year of life.

    abstract:BACKGROUND:Torque teno virus is a small chronically persisting circular negative ssDNA virus reaching near 100% prevalence. It is reported to be a marker for immune function in immunocompromised patients. The possibility of vertical maternal-fetal transmission remains controversial but incidence rate of TTV DNA in chil...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Tyschik EA,Rasskazova AS,Degtyareva AV,Rebrikov DV,Sukhikh GT

    更新日期:2018-05-30 00:00:00

  • Prevalence and resistance mutations of non-B HIV-1 subtypes among immigrants in Southern Spain along the decade 2000-2010.

    abstract:BACKGROUND:Most of the non-B HIV-1 subtypes are predominant in Sub-Saharan Africa and India although they have been found worldwide. In the last decade, immigration from these areas has increased considerably in Spain. The objective of this study was to evaluate the prevalence of non-B subtypes circulating in a cohort ...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: de Felipe B,Pérez-Romero P,Abad-Fernández M,Fernandez-Cuenca F,Martinez-Fernandez FJ,Trastoy M,Mata Rdel C,López-Cortés LF,Leal M,Viciana P,Vallejo A

    更新日期:2011-08-26 00:00:00

  • Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro.

    abstract:BACKGROUND:Human adenoviruses (Ads) have substantial potential for clinical applications in cancer patients. Conditionally replicating adenoviruses (CRAds) include oncolytic adenoviruses in which expression of the immediate early viral transactivator protein E1A is controlled by a cancer cell-selective promoter. To enh...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Fontecedro AC,Lutschg V,Eichhoff O,Dummer R,Greber UF,Hemmi S

    更新日期:2010-07-29 00:00:00

  • Human Polyomavirus JC monitoring and noncoding control region analysis in dynamic cohorts of individuals affected by immune-mediated diseases under treatment with biologics: an observational study.

    abstract:BACKGROUND:Progressive multifocal leukoencephalopathy (PML) onset, caused by Polyomavirus JC (JCPyV) in patients affected by immune-mediated diseases during biological treatment, raised concerns about the safety profile of these agents. Therefore, the aims of this study were the JCPyV reactivation monitoring and the no...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Bellizzi A,Anzivino E,Rodio DM,Cioccolo S,Scrivo R,Morreale M,Pontecorvo S,Ferrari F,Di Nardo G,Nencioni L,Carluccio S,Valesini G,Francia A,Cucchiara S,Palamara AT,Pietropaolo V

    更新日期:2013-09-30 00:00:00

  • A mobile genetic element with unknown function found in distantly related viruses.

    abstract:BACKGROUND:The genetic element s2m seems to represent one of very few examples of mobile genetic elements in viruses. The function remains obscure and a scattered taxonomical distribution has been reported by numerous groups. METHODS:We have searched GenBank in order to identify all viral accessions that have s2m(-lik...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Tengs T,Kristoffersen AB,Bachvaroff TR,Jonassen CM

    更新日期:2013-04-25 00:00:00

  • Characteristics and PD-1 expression of peripheral CD4+CD127loCD25hiFoxP3+ Treg cells in chronic HCV infected-patients.

    abstract:BACKGROUND:Both regulatory T cells (Tregs) and PD-1/PD-L1 pathway were critically involved in HCV viral persistence. However, the association between them was not well investigated. Herein, we aimed to investigate the distributional profiles of Tregs subsets and association between PD-1 expression on these subsets and ...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Shen T,Zheng J,Liang H,Xu C,Chen X,Zhang T,Xu Q,Lu F

    更新日期:2011-06-07 00:00:00

  • Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells.

    abstract:BACKGROUND:Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian tiger mosquitoes. To study the processing of envelope proteins E1 and...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Metz SW,Geertsema C,Martina BE,Andrade P,Heldens JG,van Oers MM,Goldbach RW,Vlak JM,Pijlman GP

    更新日期:2011-07-15 00:00:00

  • Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case.

    abstract:BACKGROUND:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS:We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, k...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Li S,Li Y,Wang Q,Yu X,Liu M,Xie H,Qian L,Ye L,Yang Z,Zhang J,Zhu H,Zhang W

    更新日期:2018-05-30 00:00:00

  • Differential pathogenicity of two different recombinant PVY(NTN) isolates in Physalis floridana is likely determined by the coat protein gene.

    abstract::A previous study has identified two types of recombinant variants of Potato virus Y strain NTN (PVY(NTN)) in China and sequenced the complete genome of the variant PVY(NTN)-HN2. In this study, the complete genome of isolate PVY(NTN)-HN1 was fully sequenced and analyzed. The most striking difference between the two var...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Hu X,Nie X,He C,Xiong X

    更新日期:2011-05-07 00:00:00

  • Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States.

    abstract:BACKGROUND:Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have mad...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Chen Q,Wang L,Zheng Y,Zhang J,Guo B,Yoon KJ,Gauger PC,Harmon KM,Main RG,Li G

    更新日期:2018-05-25 00:00:00

  • Local production of CCL3, CCL11, and IFN-γ correlates with disease severity in murine parainfluenza virus infection.

    abstract:BACKGROUND:Using a murine model of parainfluenza virus infection (mPIV1 or Sendai virus; SeV), we compared the inflammatory responses to lethal and sub-lethal infections in inbred DBA/2 mice. METHODS:Mice were intranasally inoculated with either 1.6×10(3) or 1.6×10(5) infectious units (IU) of SeV or diluent control. C...

    journal_title:Virology journal

    pub_type: 杂志文章


    authors: Suryadevara M,Bonville CA,Rosenberg HF,Domachowske JB

    更新日期:2013-12-21 00:00:00