Abstract:
:A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10² DNA copies/μL, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.
journal_name
Virol Jjournal_title
Virology journalauthors
Song C,Zhu C,Zhang C,Cui Sdoi
10.1186/1743-422X-7-353subject
Has Abstractpub_date
2010-12-02 00:00:00pages
353issn
1743-422Xpii
1743-422X-7-353journal_volume
7pub_type
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