Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene.

Abstract:

:A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10² DNA copies/μL, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.

journal_name

Virol J

journal_title

Virology journal

authors

Song C,Zhu C,Zhang C,Cui S

doi

10.1186/1743-422X-7-353

subject

Has Abstract

pub_date

2010-12-02 00:00:00

pages

353

issn

1743-422X

pii

1743-422X-7-353

journal_volume

7

pub_type

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