Abstract:
BACKGROUND:The Hepatitis B Virus X protein (HBx) plays a major role in hepatocellular carcinoma (HCC) development, however, its contribution to tumor invasion and metastasis has not been established so far. Heat shock protein 90 alpha (HSP90alpha) isoform is an ATP-dependent molecular chaperone that maintains the active conformation of client oncoproteins in cancer cells, which is abundantly expressed in HCC, especially in hepatitis B virus (HBV)-related tumors, might be involved in tumor progression. METHODS:The levels of HSP90alpha, extracellular signal-regulated kinase 1/2 (ERK1/2), phosphorylated ERK1/2 (p-ERK1/2) and c-Myc in HBx-transfected HepG2 cells were determined by western blots analysis. The endogenous ERKs activity was demonstrated by ELISA assay. The regulation of c-Myc-mediated HSP90 alpha promoter transactivation by HBx was evaluated through electrophoretic mobility shift analysis (EMSA). The c-Myc-mediated HSP90alpha transcription was analysed by promoter assay. The HBx-expressing cells were transfected with specific small interference RNA (siRNA) against c-Myc. The in vitro invasion potentials of cells were evaluated by Transwell cell invasion assay. RESULTS:HBx induces HSP90alpha expression at the transcription level. The induction effect of HBx was inhibited after treatment with c-Myc inhibitor, 10058-F4. In addition, the luciferase activity of the HSP90alpha promoter analysis revealed that the HBx is directly involved in the c-Myc-mediated transcriptional activation of HSP90alpha. Furthermore, HBx induces c-Myc expression by activation of Ras/Raf/ERK1/2 cascades, which in turn results in activation of the c-Myc-mediated HSP90alpha promoter and subsequently up-regulation of the HSP90alpha expression. Overexpression of HSP90alpha in HBx-transfected cells enhances tumor cells invasion. siRNA-mediated c-Myc knockdown in HBx-transfected cells significantly suppressed HSP90alpha expression and cells invasion in vitro. CONCLUSION:These results demonstrate the ability of HBx to promote tumor cells invasion by a mechanism involving the up-regulation of HSP90alpha and provide new insights into the mechanism of action of HBx and its involvement in tumor metastasis and recurrence of HCC.
journal_name
Virol Jjournal_title
Virology journalauthors
Li W,Miao X,Qi Z,Zeng W,Liang J,Liang Zdoi
10.1186/1743-422X-7-45subject
Has Abstractpub_date
2010-02-20 00:00:00pages
45issn
1743-422Xpii
1743-422X-7-45journal_volume
7pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. METHODS:The expression...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1220-y
更新日期:2019-10-07 00:00:00
abstract:BACKGROUND:The emergence of influenza viruses resistant to anti-influenza drugs is a threat to global public health. The Korea Centers for Disease Control and Prevention operates the Korea Influenza and Respiratory Viruses Surveillance System (KINRESS) to monitor epidemics of influenza and Severe Acute Respiratory Infe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01375-1
更新日期:2020-07-06 00:00:00
abstract:BACKGROUND:Tanganya virus (TGNV), the only shrew-associated hantavirus reported to date from sub-Saharan Africa, is harbored by the Therese's shrew (Crocidura theresae), and is phylogenetically distinct from Thottapalayam virus (TPMV) in the Asian house shrew (Suncus murinus) and Imjin virus (MJNV) in the Ussuri white-...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-373
更新日期:2011-07-28 00:00:00
abstract:BACKGROUND:Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. METHODS:In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important sw...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1199-4
更新日期:2019-07-31 00:00:00
abstract:BACKGROUND:Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread. METHODS:The study population consis...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-014-0194-z
更新日期:2014-11-22 00:00:00
abstract:BACKGROUND:Dengue has emerged as the most significant of arboviral diseases in the 21st century. It is endemic to >100 tropical and sub-tropical countries around the world placing an estimated 3.6 billion people at risk. It is caused by four genetically similar but antigenically distinct, serotypes of dengue viruses. T...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0248-x
更新日期:2015-02-08 00:00:00
abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01353-7
更新日期:2020-06-22 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV) infection is a serious health problem worldwide. Treatment recommendation and response are mainly indicated by viral load, e antigen (HBeAg) seroconversion, and ALT levels. The S antigen (HBsAg) seroconversion is much less frequent. Since HBeAg can be negative in the presence of high ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-93
更新日期:2007-09-24 00:00:00
abstract:BACKGROUND:Crimean-Congo Hemorrhagic Fever virus (CCHFV), a member of the genus Nairovirus, family Bunyaviridae, is a tick-borne pathogen causing severe disease in humans. To better understand the CCHFV life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-42
更新日期:2005-04-25 00:00:00
abstract:BACKGROUND:Fast, reliable and easy to handle methods are required to facilitate urgently needed point-of-care testing (POCT) in the current coronavirus pandemic. Life-threatening severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread all over the world, infecting more than 33,500,000 people and...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01435-6
更新日期:2020-10-21 00:00:00
abstract:BACKGROUND:Public health risks associated to infection by human coronaviruses remain considerable and vaccination is a key option for preventing the resurgence of severe acute respiratory syndrome coronavirus (SARS-CoV). We have previously reported that antibodies elicited by a SARS-CoV vaccine candidate based on recom...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-82
更新日期:2014-05-06 00:00:00
abstract:BACKGROUND:hyaluronic acid (HA), a non-sulphated glycosaminoglycan, is present in synovial fluid, vitreous humour serum and many connective tissues. Pharmaceutical preparations of HA are used in clinical practice for wound healing, joint pain, kerato-conjunctivitis, asthma, mouth care, oesophageal-reflux, and gastritis...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-141
更新日期:2011-03-25 00:00:00
abstract:BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-38
更新日期:2012-02-15 00:00:00
abstract:BACKGROUND:Due to the high prevalence of viral infections having no specific treatment and the constant appearance of resistant viral strains, the development of novel antiviral agents is essential. The aim of this study was to evaluate the antiviral activity against bovine viral diarrhea virus, herpes simplex virus ty...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-245
更新日期:2013-07-27 00:00:00
abstract::We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that th...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-6-95
更新日期:2009-07-07 00:00:00
abstract:BACKGROUND:We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has a mixture of features normally associated with other specific geminivirus genera...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-36
更新日期:2009-03-25 00:00:00
abstract:BACKGROUND:Lymphocytes provide invaluable whistle blowers of changes due to infections. We use the information registered by these cells using their mRNAs as they encounter the pathogen to develop patterns of expression that correspond to that specific pathogen. Venezuelan equine encephalitis (VEE) is a mosquito-borne ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-82
更新日期:2007-08-29 00:00:00
abstract::Hepatitis C virus (HCV) is endemic worldwide, and it causes cirrhosis and other complications that often lead to death; nevertheless, our knowledge of the disease and its mechanisms is limited. HCV is most common in underdeveloped nations, including many in Africa and Asia. The virus is usually transmitted by parenter...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-9-57
更新日期:2012-03-02 00:00:00
abstract:BACKGROUND:Since its first appearance in the USA in 1999, West Nile virus (WNV) has spread in the Western hemisphere and continues to represent an important public health concern. In the absence of effective treatment, there is a medical need for the development of a safe and efficient vaccine. Live attenuated WNV vacc...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-146
更新日期:2010-07-06 00:00:00
abstract:BACKGROUND:The global spread of the highly pathogenic avian influenza H5N1 virus has stimulated interest in a better understanding of the mechanisms of H5N1 dispersal, including the potential role of migratory birds as carriers. Although wild birds have been found dead during H5N1 outbreaks, evidence suggests that othe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-71
更新日期:2008-06-04 00:00:00
abstract:BACKGROUND:Ebola viruses (EBOVs) cause severe hemorrhagic fever with a high mortality rate. At present, there are no licensed vaccines or efficient therapies to combat EBOV infection. Previous studies have shown that both humoral and cellular immune responses are crucial for controlling Ebola infection. CD8+ T cells pl...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-111
更新日期:2012-06-13 00:00:00
abstract:BACKGROUND:In China, more than 20 million patients with chronic hepatitis B need antiviral treatment. Side effects of antiviral treatment such as renal complications can be problematic, particularly in an aging population. METHODS:The data were retrospectively extracted from the hospital medical charts of five centers...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-021-01491-6
更新日期:2021-01-13 00:00:00
abstract:BACKGROUND:Chronic hepatitis C virus (HCV) genotype (GT) 3 infection with advanced liver disease has emerged as a challenging to treat by direct-acting antivirals (DAAs), but the efficacy of DAAs in Chinese HCV-GT3 patients is rarely reported. This study aimed to analyze the efficacy of sofosbuvir (SOF)-based regimens ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1066-8
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:The mint family (Lamiaceae) produces a wide variety of constituents with medicinal properties. Several family members have been reported to have antiviral activity, including lemon balm (Melissa officinalis L.), sage (Salvia spp.), peppermint (Mentha×piperita L.), hyssop (Hyssopus officinalis L.), basil (Oci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-188
更新日期:2011-04-23 00:00:00
abstract:BACKGROUND:In adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other adenovirus capsid proteins and leads to a productive infection. Altho...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0309-1
更新日期:2015-05-22 00:00:00
abstract::Flaviviruses are small viruses with single-stranded RNA, which include the yellow fever virus, dengue virus, West Nile virus, Japanese encephalitis virus, tick-borne encephalitis virus, and Zika virus; and are causal agents of the most important emerging diseases that have no available treatment to date. In recent yea...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-017-0761-1
更新日期:2017-05-15 00:00:00
abstract:BACKGROUND:Reporting new cases of enterovirus (EV)-D68-associated acute flaccid myelitis (AFM) is essential to understand how the virus causes neurological damage and to characterize EV-D68 strains associated with AFM. CASE PRESENTATION:A previously healthy 4-year-old boy presented with sudden weakness and limited mob...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0678-0
更新日期:2017-01-11 00:00:00
abstract:BACKGROUND:Chronic viral hepatitis B remains a global public health concern. Currently, several drugs, such as lamivudine and telbivudine, are recommended for treatment of patients with chronic hepatitis B. However, there are no conclusive results on the comparison of the efficacy of lamivudine (LAM) and telbivudine (L...
journal_title:Virology journal
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1743-422X-7-211
更新日期:2010-09-03 00:00:00
abstract::HIV expressed in genital secretions provides the inoculum from which transmitting variants are selected, both in sexual transmission and mother-to-infant transmission during partuition. Characterization of HIV levels and genotypes found in vaginal secretions and the impact of anti-retroviral therapy (ART) on this viru...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-87
更新日期:2008-07-29 00:00:00
abstract:BACKGROUND:Human pegivirus-1 (HPgV-1) is a member of the Flaviviridae family whose genomic organization and mode of cellular entry is similar to that of hepatitis C virus (HCV). The E2 glycoprotein of HPgV-1 is the principle mediator in the virus-cell interaction and as such harbors most of HPgV-1's antigenic determina...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0837-y
更新日期:2017-08-31 00:00:00