当前位置： SCI文献检索 > Virology Journal期刊下所有文献 > Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Abstract：

BACKGROUND:Fast, reliable and easy to handle methods are required to facilitate urgently needed point-of-care testing (POCT) in the current coronavirus pandemic. Life-threatening severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread all over the world, infecting more than 33,500,000 people and killing over 1 million of them as of October 2020. Infected individuals without any symptoms might still transfer the virus to others underlining the extraordinary transmissibility of this new coronavirus. In order to identify early infections effectively, treat patients on time and control disease spreading, rapid, accurate and onsite testing methods are urgently required. RESULTS:Here we report the development of a loop-mediated isothermal amplification (LAMP) based method to detect SARS-CoV-2 genes ORF8 and N directly from pharyngeal swab samples. The established reverse transcription LAMP (RT-LAMP) assay detects SARS-CoV-2 directly from pharyngeal swab samples without previous time-consuming and laborious RNA extraction. The assay is sensitive and highly specific for SARS-CoV-2 detection, showing no cross reactivity when tested on 20 other respiratory pathogens. The assay is 12 times faster and 10 times cheaper than routine reverse transcription real-time polymerase chain reaction, depending on the assay used. CONCLUSION:The fast and easy to handle RT-LAMP assay amplifying specifically the genomic regions ORF8 and N of SARS-CoV-2 is ideally suited for POCT at e.g. railway stations, airports or hospitals. Given the current pandemic situation, rapid, cost efficient and onsite methods like the here presented RT-LAMP assay are urgently needed to contain the viral spread.

journal_name

Virol J

journal_title

Virology journal

authors

Mautner L,Baillie CK,Herold HM,Volkwein W,Guertler P,Eberle U,Ackermann N,Sing A,Pavlovic M,Goerlich O,Busch U,Wassill L,Huber I,Baiker A

doi

10.1186/s12985-020-01435-6

subject

Has Abstract

pub_date

2020-10-21 00:00:00

pages

160

issue

1

issn

1743-422X

pii

10.1186/s12985-020-01435-6

journal_volume

17

pub_type

杂志文章

相关文献

Virology Journal文献大全
  • Vaccination induced antibodies to recombinant avian influenza A virus M2 protein or synthetic M2e peptide do not bind to the M2 protein on the virus or virus infected cells.

    abstract：BACKGROUND:Influenza viruses are characterized by their highly variable surface proteins HA and NA. The third surface protein M2 is a nearly invariant protein in all Influenza A strains. Despite extensive studies in other animal models, this study is the first to describe the use of recombinant M2 protein and a peptide...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-10-206

    authors： Swinkels WJ,Hoeboer J,Sikkema R,Vervelde L,Koets AD

    更新日期：2013-06-24 00:00:00

  • Interactions of viruses in Cowpea: effects on growth and yield parameters.

    abstract：:The study was carried out to investigate the effects of inoculating three cowpea cultivars: "OLO II", "OLOYIN" and IT86D-719 with three unrelated viruses: Cowpea aphid-borne mosaic virus (CABMV), genus Potyvirus, Cowpea mottle virus (CMeV), genus Carmovirus and Southern bean mosaic virus (SBMV), genus Sobemovirus sing...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-4-15

    authors： Kareem KT,Taiwo MA

    更新日期：2007-02-08 00:00:00

  • Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.

    abstract：BACKGROUND:Human T-cell Leukemia Virus type 1 (HTLV-1) is the etiological agent of tropical spastic paraparesis/HTLV-associated myelopathy (HAM/TSP) that can be identified in around 0.25%-3.8% of the infected population. Disease progression can be monitored by the proviral load and may depend on genetic factors, howeve...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-10-75

    authors： Mota-Miranda AC,Barreto FK,Amarante MF,Batista E,Monteiro-Cunha JP,Farre L,Galvão-Castro B,Alcantara LC

    更新日期：2013-03-06 00:00:00

  • Intramuscular immunization of mice with live influenza virus is more immunogenic and offers greater protection than immunization with inactivated virus.

    abstract：BACKGROUND:Influenza virus continues to cause significant hospitalization rates in infants and young children. A 2-dose regime of trivalent inactivated vaccine is required to generate protective levels of hemagglutination inhibiting (HAI) antibodies. A vaccine preparation with enhanced immunogenicity is therefore desir...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-251

    authors： Harris K,Ream R,Gao J,Eichelberger MC

    更新日期：2011-05-21 00:00:00

  • Comparative analysis of Panicum streak virus and Maize streak virus diversity, recombination patterns and phylogeography.

    abstract：BACKGROUND:Panicum streak virus (PanSV; Family Geminiviridae; Genus Mastrevirus) is a close relative of Maize streak virus (MSV), the most serious viral threat to maize production in Africa. PanSV and MSV have the same leafhopper vector species, largely overlapping natural host ranges and similar geographical distribut...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-6-194

    authors： Varsani A,Monjane AL,Donaldson L,Oluwafemi S,Zinga I,Komba EK,Plakoutene D,Mandakombo N,Mboukoulida J,Semballa S,Briddon RW,Markham PG,Lett JM,Lefeuvre P,Rybicki EP,Martin DP

    更新日期：2009-11-10 00:00:00

  • Prevalence, type and concentration of human enterovirus and parechovirus in cerebrospinal fluid samples of pediatric patients over a 10-year period: a retrospective study.

    abstract：BACKGROUND:Human enterovirus (EV) and parechovirus (HPeV) are significant causes of encephalitis and meningitis in children. The aim of this study was to determine the prevalence, type and viral RNA concentration of EV and HPeV in cerebrospinal fluid (CSF) samples in an unselected cohort of patients <18 years admitted ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-015-0427-9

    authors： Vollbach S,Müller A,Drexler JF,Simon A,Drosten C,Eis-Hübinger AM,Panning M

    更新日期：2015-11-25 00:00:00

  • Characterization of neutralizing epitopes within the major capsid protein of human papillomavirus type 33.

    abstract：BACKGROUND:Infections with papillomaviruses induce type-specific immune responses, mainly directed against the major capsid protein, L1. Based on the propensity of the L1 protein to self-assemble into virus-like particles (VLPs), type-specific vaccines have already been developed. In order to generate vaccines that tar...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-3-83

    authors： Roth SD,Sapp M,Streeck RE,Selinka HC

    更新日期：2006-10-02 00:00:00

  • Antenatal HIV-1 RNA load and timing of mother to child transmission; a nested case-control study in a resource poor setting.

    abstract：OBJECTIVE:To determine HIV-1 RNA load during the third trimester of pregnancy and evaluate its effect on in utero and intra-partum/postpartum transmissions in a breastfeeding population. DESIGN:A nested case-control study within a PMTCT cohort of antiretroviral therapy naive pregnant women and their infants. METHODS:...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-176

    authors： Duri K,Gumbo FZ,Kristiansen KI,Kurewa NE,Mapingure MP,Rusakaniko S,Chirenje MZ,Muller F,Stray-Pedersen B

    更新日期：2010-08-02 00:00:00

  • Origin of measles virus: divergence from rinderpest virus between the 11th and 12th centuries.

    abstract：:Measles, caused by measles virus (MeV), is a common infection in children. MeV is a member of the genus Morbillivirus and is most closely related to rinderpest virus (RPV), which is a pathogen of cattle. MeV is thought to have evolved in an environment where cattle and humans lived in close proximity. Understanding th...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-52

    authors： Furuse Y,Suzuki A,Oshitani H

    更新日期：2010-03-04 00:00:00

  • Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India: evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains.

    abstract：BACKGROUND:During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). OBJECTIVE:To characterize full genome of the H1N2 influenza virus. METHODS:F...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-233

    authors： Mukherjee TR,Agrawal AS,Chakrabarti S,Chawla-Sarkar M

    更新日期：2012-10-11 00:00:00

  • Whole-genome sequencing of human Pegivirus variant from an Egyptian patient co-infected with hepatitis C virus: a case report.

    abstract：BACKGROUND:Human pegivirus (HPgV) is structurally similar to hepatitis C virus (HCV) and was discovered 20 years ago. Its distribution, natural history and exact rule of this viral group in human hosts remain unclear. Our aim was to determine, by deep next-generation sequencing (NGS), the entire genome sequence of HPgV...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-019-1242-5

    authors： Soliman HK,Abouelhoda M,El Rouby MN,Ahmed OS,Esmat G,Hassan ZK,Hafez MM,Mehaney DA,Selvaraju M,Darwish RK,Osman YA,Zekri AN

    更新日期：2019-11-11 00:00:00

  • A mobile genetic element with unknown function found in distantly related viruses.

    abstract：BACKGROUND:The genetic element s2m seems to represent one of very few examples of mobile genetic elements in viruses. The function remains obscure and a scattered taxonomical distribution has been reported by numerous groups. METHODS:We have searched GenBank in order to identify all viral accessions that have s2m(-lik...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-10-132

    authors： Tengs T,Kristoffersen AB,Bachvaroff TR,Jonassen CM

    更新日期：2013-04-25 00:00:00

  • Defining viral species: making taxonomy useful.

    abstract：:Virus taxonomy at present is best characterized as a categorization of convenience, without a firm basis in the principles of evolutionary biology. Specifically, virus species definitions appear to depend more on tradition and popular opinion among virologists than on firm, quantitative biological evidence. I suggest ...

    journal_title：Virology journal

    pub_type： 信件

    doi：10.1186/1743-422X-11-131

    authors： Peterson AT

    更新日期：2014-07-23 00:00:00

  • Current status and future directions in the management of chronic hepatitis C.

    abstract：:Hepatitis C virus (HCV) is endemic worldwide, and it causes cirrhosis and other complications that often lead to death; nevertheless, our knowledge of the disease and its mechanisms is limited. HCV is most common in underdeveloped nations, including many in Africa and Asia. The virus is usually transmitted by parenter...

    journal_title：Virology journal

    pub_type： 杂志文章,评审

    doi：10.1186/1743-422X-9-57

    authors： Aman W,Mousa S,Shiha G,Mousa SA

    更新日期：2012-03-02 00:00:00

  • A parallel genome-wide RNAi screening strategy to identify host proteins important for entry of Marburg virus and H5N1 influenza virus.

    abstract：BACKGROUND:Genome-wide RNAi screening has been widely used to identify host proteins involved in replication and infection of different viruses, and numerous host factors are implicated in the replication cycles of these viruses, demonstrating the power of this approach. However, discrepancies on target identification ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-015-0420-3

    authors： Cheng H,Koning K,O'Hearn A,Wang M,Rumschlag-Booms E,Varhegyi E,Rong L

    更新日期：2015-11-24 00:00:00

  • Herpes simplex virus type 2 infection triggers AP-1 transcription activity through TLR4 signaling in genital epithelial cells.

    abstract：BACKGROUND:The pattern recognition receptors (PPRs) are the earliest phase of the host defense against pathogens in genital epithelium, and toll-like receptors (TLRs) are best characterized PPRs mediating innate immune responses. Herpes simplex virus type 2 (HSV-2), a member of herpesviridae family, causes one of the m...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-018-1087-3

    authors： Lv X,Wang H,Su A,Xu S,Chu Y

    更新日期：2018-11-12 00:00:00

  • Full-length Ebola glycoprotein accumulates in the endoplasmic reticulum.

    abstract：:The Filoviridae family comprises of Ebola and Marburg viruses, which are known to cause lethal hemorrhagic fever. However, there is no effective anti-viral therapy or licensed vaccines currently available for these human pathogens. The envelope glycoprotein (GP) of Ebola virus, which mediates entry into target cells, ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-11

    authors： Bhattacharyya S,Hope TJ

    更新日期：2011-01-12 00:00:00

  • Rotavirus NSP4 is secreted from infected cells as an oligomeric lipoprotein and binds to glycosaminoglycans on the surface of non-infected cells.

    abstract：BACKGROUND:Nonstructural glycoprotein 4 (NSP4) encoded by rotavirus is the only viral protein currently believed to function as an enterotoxin. NSP4 is synthesized as an intracellular transmembrane glycoprotein and as such is essential for virus assembly. Infection of polarized Caco-2 cells with rotavirus also results ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-551

    authors： Didsbury A,Wang C,Verdon D,Sewell MA,McIntosh JD,Taylor JA

    更新日期：2011-12-20 00:00:00

  • Investigation of the immunogenicity of Zika glycan loop.

    abstract：BACKGROUND:Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked glycan on the "glycan loop...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-020-01313-1

    authors： Henderson EA,Tam CC,Cheng LW,Ngono AE,Nguyen AV,Shresta S,McGee M,Padgett H,Grill LK,Martchenko Shilman M

    更新日期：2020-03-31 00:00:00

  • Author Correction: In vitro virucidal activity of Echinaforce®, an Echinacea purpurea preparation, against coronaviruses, including common cold coronavirus 229E and SARS-CoV-2.

    abstract：:The original version of the acknowledgement unfortunately contained a mistake. ...

    journal_title：Virology journal

    pub_type： 已发布勘误

    doi：10.1186/s12985-020-01439-2

    authors： Signer J,Jonsdottir HR,Albrich WC,Strasser M,Züst R,Ryter S,Ackermann-Gäumann R,Lenz N,Siegrist D,Suter A,Schoop R,Engler OB

    更新日期：2020-11-09 00:00:00

  • The altered gut virome community in rhesus monkeys is correlated with the gut bacterial microbiome and associated metabolites.

    abstract：BACKGROUND:The gut microbiome is closely associated with the health of the host; although the interaction between the bacterial microbiome and the whole virome has rarely been studied, it is likely of medical importance. Examination of the interactions between the gut bacterial microbiome and virome of rhesus monkey wo...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-019-1211-z

    authors： Li H,Li H,Wang J,Guo L,Fan H,Zheng H,Yang Z,Huang X,Chu M,Yang F,He Z,Li N,Yang J,Wu Q,Shi H,Liu L

    更新日期：2019-08-19 00:00:00

  • Classic swine fever virus NS2 protein leads to the induction of cell cycle arrest at S-phase and endoplasmic reticulum stress.

    abstract：BACKGROUND:Classical swine fever (CSF) caused by virulent strains of Classical swine fever virus (CSFV) is a haemorrhagic disease of pigs, characterized by disseminated intravascular coagulation, thrombocytopoenia and immunosuppression, and the swine endothelial vascular cell is one of the CSFV target cells. In this re...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-4

    authors： Tang QH,Zhang YM,Fan L,Tong G,He L,Dai C

    更新日期：2010-01-11 00:00:00

  • Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression.

    abstract：BACKGROUND:Bovine leukemia virus (BLV), which is closely related to human T-cell leukemia virus, is the etiological agent of enzootic bovine leukosis, a disease characterized by a highly prolonged course involving persistent lymphocytosis and B-cell lymphoma. The bovine major histocompatibility complex class II region ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-019-1259-9

    authors： Bai L,Takeshima SN,Sato M,Davis WC,Wada S,Kohara J,Aida Y

    更新日期：2019-12-16 00:00:00

  • Seewis virus, a genetically distinct hantavirus in the Eurasian common shrew (Sorex araneus).

    abstract：:More than 20 years ago, hantaviral antigens were reported in tissues of the Eurasian common shrew (Sorex araneus), Eurasian water shrew (Neomys fodiens) and common mole (Talpa europea), suggesting that insectivores, or soricomorphs, might serve as reservoirs of unique hantaviruses. Using RT-PCR, sequences of a genetic...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-4-114

    authors： Song JW,Gu SH,Bennett SN,Arai S,Puorger M,Hilbe M,Yanagihara R

    更新日期：2007-10-30 00:00:00

  • Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0.

    abstract：BACKGROUND:Herpes simplex viruses (HSVs) rapidly shut off macromolecular synthesis in host cells. In contrast, global microarray analyses have shown that HSV infection markedly up-regulates a number of host cell genes that may play important roles in HSV-host cell interactions. To understand the regulatory mechanisms i...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-257

    authors： Kamakura M,Goshima F,Luo C,Kimura H,Nishiyama Y

    更新日期：2011-05-25 00:00:00

  • Systemic virus distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virus.

    abstract：BACKGROUND:Avian influenza virus (AIV) is classified into two pathotypes, low pathogenic (LP) and high pathogenic (HP), based on virulence in chickens.Differences in pathogenicity between HPAIV and LPAIV might eventually be related to specific characteristics of strains, tissue tropism and host responses. METHODS:To s...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-61

    authors： Post J,Burt DW,Cornelissen JB,Broks V,van Zoelen D,Peeters B,Rebel JM

    更新日期：2012-03-06 00:00:00

  • Over-expression of mitochondrial antiviral signaling protein inhibits coxsackievirus B3 infection by enhancing type-I interferons production.

    abstract：BACKGROUND:Recent studies have revealed that Mitochondrial Antiviral Signaling (MAVS) protein plays an essential role in the inhibition of viral infection through type I interferon (IFN) pathway. It has been shown that 3C (pro) cysteine protease of coxsackievirus B3 (CVB3) cleaves MAVS to inhibit type I IFNs induction....

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-312

    authors： Zhang QM,Song WQ,Li YJ,Qian J,Zhai AX,Wu J,Li AM,He JM,Zhao JY,Yu X,Wei LL,Zhang FM

    更新日期：2012-12-19 00:00:00

  • Epidemics of viral meningitis caused by echovirus 6 and 30 in Korea in 2008.

    abstract：BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-38

    authors： Kim HJ,Kang B,Hwang S,Hong J,Kim K,Cheon DS

    更新日期：2012-02-15 00:00:00

  • RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite.

    abstract：BACKGROUND:Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus (family Geminiviridae) is responsible for heavy yield losses for tomato production around the globe. In Oman at least five distinct begomoviruses cause disease in tomato, including TYLCV. Unusually, TYLCV infections in Oman are sometimes associ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-015-0263-y

    authors： Ammara Ue,Mansoor S,Saeed M,Amin I,Briddon RW,Al-Sadi AM

    更新日期：2015-03-04 00:00:00

  • Genetic lesions within the 3a gene of SARS-CoV.

    abstract：:A series of frameshift mutations within the 3a gene has been observed in culture-derived severe acute respiratory syndrome coronavirus (SARS-CoV). We report here that viral RNA from clinical samples obtained from SARS-CoV infected patients also contains a heterogeneous population of wild-type and mutant 3a transcripts...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-2-51

    authors： Tan TH,Barkham T,Fielding BC,Chou CF,Shen S,Lim SG,Hong W,Tan YJ

    更新日期：2005-06-20 00:00:00