Abstract:
:Mycoviruses infect a large number of diverse fungal species, but considering their prevalence, relatively few high-quality genome sequences have been determined. Many mycoviruses have linear double-stranded RNA genomes, which makes it technically challenging to ascertain their nucleotide sequence using conventional sequencing methods. Different specialist methodologies have been developed for the extraction of double-stranded RNAs from fungi and the subsequent synthesis of cDNAs for cloning and sequencing. However, these methods are often labor-intensive, time-consuming, and can require several days to produce cDNAs from double-stranded RNAs. Here, we describe a comprehensive method for the rapid extraction and sequencing of dsRNAs derived from yeasts, using short-read next generation sequencing. This method optimizes the extraction of high-quality double-stranded RNAs from yeasts and 3' polyadenylation for the initiation of cDNA synthesis for next-generation sequencing. We have used this method to determine the sequence of two mycoviruses and a double-stranded RNA satellite present within a single strain of the model yeast Saccharomyces cerevisiae. The quality and depth of coverage was sufficient to detect fixed and polymorphic mutations within viral populations extracted from a clonal yeast population. This method was also able to identify two fixed mutations within the alpha-domain of a variant K1 killer toxin encoded on a satellite double-stranded RNA. Relative to the canonical K1 toxin, these newly reported mutations increased the cytotoxicity of the K1 toxin against a specific species of yeast.
journal_name
Virusesjournal_title
Virusesauthors
Crabtree AM,Kizer EA,Hunter SS,Van Leuven JT,New DD,Fagnan MW,Rowley PAdoi
10.3390/v11010070subject
Has Abstractpub_date
2019-01-16 00:00:00issue
1issn
1999-4915pii
v11010070journal_volume
11pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Influenza A viruses (IAVs) are a major cause of respiratory illness and are responsible for yearly epidemics associated with more than 500,000 annual deaths globally. Novel IAVs may cause pandemic outbreaks and zoonotic infections with, for example, highly pathogenic avian influenza virus (HPAIV) of the H5N1 and H7N9 ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11020171
更新日期:2019-02-20 00:00:00
abstract::Evidence that Hepatitis D virus (HDV) genotype is involved in HDV infection pathogenesis is increasing. Indeed, HDV genotypes have been shown to be linked to different outcomes in terms of liver fibrosis and treatment response. Herein, we show that the promising HDVdb genotyping tool available online can lead to wrong...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12101066
更新日期:2020-09-23 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) was first isolated in 2012, and circulated worldwide with high mortality. The continual outbreaks of MERS-CoV highlight the importance of developing antiviral therapeutics. Here, we rationally designed a novel fusion inhibitor named MERS-five-helix bundle (ME...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9090255
更新日期:2017-09-14 00:00:00
abstract::The Megalocytivirus genus includes three genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV), and has caused mass mortalities in various marine and freshwater fish species in East and Southeast Asia. Of the three genotypes, TRBIV-l...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12060681
更新日期:2020-06-24 00:00:00
abstract::The genomes of polyomaviruses are characterized by their tripartite organization with an early region, a late region and a noncoding control region (NCCR). The early region encodes proteins involved in replication and transcription of the viral genome, while expression of the late region generates the capsid proteins....
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12121406
更新日期:2020-12-07 00:00:00
abstract::Porcine circovirus 3 (PCV3) has been identified as a putative swine pathogen with a subset of infections resulting in stillborn and mummified fetuses, encephalitis and myocarditis in perinatal, and periarteritis in growing pigs. Three PCV3 isolates were isolated from weak-born piglets or elevated stillborn and mummifi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020219
更新日期:2020-02-16 00:00:00
abstract::Influenza A virus (IAV) in swine, so-called swine influenza A virus (swIAV), causes respiratory illness in pigs around the globe. In Danish pig herds, a H1N2 subtype named H1N2dk is one of the main circulating swIAV. In this cohort study, the infection dynamic of swIAV was evaluated in a Danish pig herd by sampling an...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12091013
更新日期:2020-09-10 00:00:00
abstract::Arthropod-borne flaviviruses are human pathogens of global medical importance, against which no effective small molecule-based antiviral therapy has currently been reported. Arbidol (umifenovir) is a broad-spectrum antiviral compound approved in Russia and China for prophylaxis and treatment of influenza. This compoun...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10040184
更新日期:2018-04-10 00:00:00
abstract::Efforts towards developing a vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV) have yielded promising results. Utilizing a variety of platforms, several vaccine approaches have shown efficacy in animal models and begun to enter clinical trials. In this review, we summarize the current progress toward...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11010074
更新日期:2019-01-17 00:00:00
abstract::Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain⁻Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZI...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11020092
更新日期:2019-01-22 00:00:00
abstract::The Human immunodeficiency virus-1 (HIV-1) accessory protein Vpu modulates numerous proteins, including the host proteins CD4 and BST-2/tetherin. Vpu interacts with the Skp, Cullin, F-Box (SCF) ubiquitin ligase through interactions with the F-Box protein βTrCP (1 and/or 2). This interaction is dependent on phosphoryla...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10100573
更新日期:2018-10-19 00:00:00
abstract::Short or damaged telomeres have been implicated in degenerative conditions. We hypothesized that analysis of telomere length (TL) in human T-cell lymphotropic virus (HTLV) infection and HTLV-associated neuropathy might provide clues to the etiology of HTLV-associated disease and viral dynamics. A subset of 45 human T-...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8080221
更新日期:2016-08-11 00:00:00
abstract::Paramyxoviruses comprise a large number of diverse viruses which in part give rise to severe diseases in affected hosts. A new genotype of feline morbillivirus, tentatively named feline morbillivirus genotype 2 (FeMV-GT2), was isolated from urine of cats with urinary tract diseases. Whole genome sequencing showed abou...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11020146
更新日期:2019-02-09 00:00:00
abstract::HIV-1 Gag polyprotein orchestrates the assembly of viral particles. Its C-terminus consists of the nucleocapsid (NC) domain that interacts with nucleic acids, and p1 and p6, two unstructured regions, p6 containing the motifs to bind ALIX, the cellular ESCRT factor TSG101 and the viral protein Vpr. The processing of Ga...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12101109
更新日期:2020-09-29 00:00:00
abstract::The initial step of viral infection is the binding of a virus onto the host cell surface. This first viral-host interaction would determine subsequent infection steps and the fate of the entire infection process. A basic understating of the underlining mechanism of initial virus-host binding is a prerequisite for esta...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4113162
更新日期:2012-11-15 00:00:00
abstract::Circoviruses infect a variety of animal species and have small (~1.8-2.2 kb) circular single-stranded DNA genomes. Recently a penguin circovirus (PenCV) was identified associated with an Adélie Penguin (Pygoscelis adeliae) with feather disorder and in the cloacal swabs of three asymptomatic Adélie Penguins at Cape Cro...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12080858
更新日期:2020-08-06 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is a complex retrovirus that infects CD4+ T cells and causes adult T-cell leukemia/lymphoma (ATLL) in 3%-5% of infected individuals after a long latent period. HTLV-1 Tax is a trans-activating protein that regulates viral gene expression and also modulates cellular signaling...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6103925
更新日期:2014-10-23 00:00:00
abstract::Human parechovirus (HPeV), particularly type 3 (HPeV3), is an important cause of sepsis-/meningitis-like illness in young infants. Laboratory records identified a total of ten HPeV-positive cases in Southeastern Australia between January and July 2019. The HPeV present in these cases were typed by Sanger sequencing of...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100913
更新日期:2019-10-03 00:00:00
abstract::One hundred Prunus trees, including almond (P. dulcis), apricot (P. armeniaca), nectarine (P. persica var. nucipersica), peach (P. persica), plum (P. domestica), purple leaf plum (P. cerasifera) and sweet cherry (P. avium), were selected from growing regions Australia-wide and tested for the presence of 34 viruses and...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020246
更新日期:2020-02-23 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with P...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11060544
更新日期:2019-06-11 00:00:00
abstract::The successful control of HBV infection requires an efficient expansion of distinct elements of the adaptive immune system (B cells, helper and cytotoxic T cells) that, due to the hepatotropic nature of HBV, need to operate in the liver parenchyma. In this respect, we will discuss broad features of HBV immunity in pat...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v1020091
更新日期:2009-09-01 00:00:00
abstract::The MinION sequencer is increasingly being used for the detection and outbreak surveillance of pathogens due to its rapid throughput. For RNA viruses, MinION's new direct RNA sequencing is the next significant development. Direct RNA sequencing studies are currently limited and comparisons of its diagnostic performanc...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12080801
更新日期:2020-07-25 00:00:00
abstract::The smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae), is an economically important pest of tea in Japan. Previous work showed that a fast-killing nucleopolyhedrovirus (NPV) isolated from A. orana (AdorNPV) and a slow-killing NPV isolated from A. honmai (AdhoNPV) are both infectious to A. honmai larvae...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7031271
更新日期:2015-03-18 00:00:00
abstract::The ability of the host to trigger apoptosis in infected cells is perhaps the most powerful tool by which viruses can be cleared from the host organism. To avoid elimination by this mechanism, human papillomaviruses (HPV) have developed several mechanisms that enable the cells they infect to elude both extrinsic and i...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4123831
更新日期:2012-12-18 00:00:00
abstract::The development of oncolytic viruses has led to an emerging new class of cancer therapeutics. Although the safety profile has been encouraging, the transition of oncolytic viruses to the clinical setting has been a slow process due to modifications. Therefore, a new generation of more potent oncolytic viruses needs to...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v8030072
更新日期:2016-03-07 00:00:00
abstract::Present in many cell types, non-degradative secretory autophagy is a newly discovered pathway in which autophagosomes fuse with the plasma membrane instead of lysosomes. Surprisingly, some viruses exploit secretory autophagy to exit cells non-lytically, shedding into the extracellular environment as particle populatio...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10030139
更新日期:2018-03-20 00:00:00
abstract::Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with diffe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7082815
更新日期:2015-07-27 00:00:00
abstract::Currently, only three phages that infect the medically important bacterium Clostridium difficile have been discussed by the International Committee of Viral Taxonomy (ICTV). They are all myoviruses, and have been assigned to the genus "phicd119likevirus". An additional nine phages have since been described in the lite...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7052534
更新日期:2015-05-21 00:00:00
abstract::Hantaviruses are an established cause of haemorrhagic fever with renal syndrome (HFRS) in Europe. Following a confirmed case of HFRS in the UK, in an individual residing on a farm in North Yorkshire and the Humber, a tidal estuary on the east coast of Northern England, and the subsequent isolation of a Seoul hantaviru...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v6020524
更新日期:2014-02-05 00:00:00
abstract::As more cases of COVID-19 are studied and treated worldwide, it had become apparent that the lethal and most severe cases of pneumonia are due to an out-of-control inflammatory response to the SARS-CoV-2 virus. I explored the putative causes of this specific feature through a detailed genomic comparison with the close...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12060646
更新日期:2020-06-15 00:00:00