Abstract：
BACKGROUND:Diet-induced hepatic steatosis is highly associated with nonalcoholic fatty liver disease, which is related to the development of metabolic syndrome. While advanced stage nonalcoholic hepatic steatosis and steatohepatitis (NASH) result ultimately in fibrosis and cirrhosis, the molecular basis for lipid droplet formation is poorly understood. Common pathways underlie the pathology of mammalian obesity and the zebrafish diet-induced obesity model (DIO-zebrafish) used in this study. METHODS:Our analysis involved a combination of transcriptome (DNA microarray) and proteome (two-dimensional electrophoresis) methods using liver tissue from DIO-zebrafish to find candidate genes involved in hepatic steatosis. We conducted intraperitoneal injection (i.p.) of morpholino antisense oligonucleotides (MOs) for each gene into DIO-zebrafish. We also conducted in vitro overexpression in human cells. Additionally, we examined gene expression during feeding experiments involving anti-obesity compounds, creatine and anserine. RESULTS:We found that fatty acid binding protein 3 (fabp3) and E2F transcription factors were upregulated in hepatic steatosis. E2f8 MO i.p. suppressed fabp3 expression in liver, and ameliorated hepatic steatosis. In human cells (HepG2), E2F8 overexpression promoted FABP3 expression. Additionally, co-administration of creatine and anserine suppressed obesity associated phenotypes including hepatic steatosis as indicated by e2f8 and fabp3 down regulation. CONCLUSION:We discovered that the e2f8-fabp3 axis is important in the promotion of hepatic steatosis in DIO-zebrafish. The combination of transcriptome and proteome analyses using the disease model zebrafish allow identification of novel pathways involved in human diseases.
journal_name
Nutr Metab (Lond)journal_title
Nutrition & metabolismauthors
Shimada Y,Kuninaga S,Ariyoshi M,Zhang B,Shiina Y,Takahashi Y,Umemoto N,Nishimura Y,Enari H,Tanaka Tdoi
10.1186/s12986-015-0012-7subject
Has Abstractpub_date
2015-05-20 00:00:00pages
17issn
1743-7075pii
12journal_volume
12pub_type
杂志文章abstract：: Sphingomyelin synthase (SMS) sits at the crossroads of sphingomyelin (SM), ceramide, diacylglycerol (DAG) metabolism. It utilizes ceramide and phosphatidylcholine as substrates to produce SM and DAG, thereby regulating lipid messengers which play a role in cell survival and apoptosis. Furthermore, its product SM has ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-8-89
更新日期：2011-12-14 00:00:00
abstract：: Drug-drug interactions have become an important issue in health care. It is now realized that many drug-drug interactions can be explained by alterations in the metabolic enzymes that are present in the liver and other extra-hepatic tissues. Many of the major pharmacokinetic interactions between drugs are due to hepa...
journal_title：Nutrition & metabolism
pub_type： 杂志文章,收录出版
doi：10.1186/1743-7075-5-27
更新日期：2008-10-18 00:00:00
abstract：Objective:This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to determine the effect of alpha-lipoic acid (ALA) supplementation on the inflammatory markers among patients with metabolic syndrome (MetS) and related disorders. Methods:We searched the following databases until N...
journal_title：Nutrition & metabolism
pub_type： 杂志文章,评审
doi：10.1186/s12986-018-0274-y
更新日期：2018-06-05 00:00:00
abstract：Background:The aim of this study was to investigate how the signaling pathway downstream of mTOR/S6K1 contributes to the regulation of SREBP-1c expression during lipogenesis in HepG2 cells. Methods:The model of steatosis was established using human hepatocytes HepG2 and inducting them with sodium palmitate. mTOR, S6K1...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-018-0268-9
更新日期：2018-05-02 00:00:00
abstract：BACKGROUND:Sub-optimally nourished rats show reduced growth, biochemical and physiological changes. However, no one has assessed metabolic rate adaptations in rats subjected to chronic suboptimal nutrition (CSN). In this study energy expenditure (EE; kcal/100 g body weight) and physical activity (PA; oscillations in we...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-3-11
更新日期：2006-01-31 00:00:00
abstract：Background:To investigate the association between different obesity phenotypes and the risk of incident hypertension among both genders. Methods:The study population included 3659 Iranians (men = 1540), aged ≥20 years free of hypertension at baseline. Participants were classified into six categories of body mass index...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-019-0340-0
更新日期：2019-02-27 00:00:00
abstract：BACKGROUND:A major portion of available fatty acids for adipocyte uptake is derived from lipoprotein lipase (LPL)-mediated hydrolysis of circulating lipoprotein particles. In vivo studies aimed at identifying the precise role of adipocyte-derived LPL in fat storage function of adipose tissue have been unable to provide...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-4-22
更新日期：2007-10-30 00:00:00
abstract：Background:Consumption of diet high in soy products is suggested to contribute to lower prostate cancer incidence in Asian men. But little has been known about the influences of dietary patterns on gut microbiota and microbiota-mediated isoflavone metabolism. Here, we determined the influences of western pattern diet o...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-019-0351-x
更新日期：2019-04-11 00:00:00
abstract：BACKGROUND:Holotranscobalamin, cobalamin-saturated transcobalamin, is the minor fraction of circulating cobalamin (vitamin B12), which is available for cellular uptake and hence is physiologically relevant. Currently, no method allows simple, direct quantification of holotranscobalamin. We now report on the identificat...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-3-3
更新日期：2006-01-04 00:00:00
abstract：BACKGROUND:The aim of the present study was to examine the effects of onion as functional ingredient on the oxidative status, lipoprotein levels (total cholesterol-TC, HDL-C, LDL-C), triacylglycerides (TAG) and vascular reactivity of mesenteric arteries in hypercholesterolemic Wistar rats. METHODS:Twenty-four animals ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-11-57
更新日期：2014-12-23 00:00:00
abstract：Background:Trehalose is well known as a functional disaccharide with anti-metabolic activities such as suppression of adipocyte hypertrophy in mice and alleviation of impaired glucose tolerance in humans. Recently, a new type of adipocyte beige cells, involved in so-called white adipocyte tissue (WAT) browning, has rec...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-019-0373-4
更新日期：2019-07-16 00:00:00
abstract：BACKGROUND:Protein energy malnutrition is commonly associated with immune dysfunctions and is a major factor in susceptibility to infectious diseases. METHODS:In this study, we evaluated the impact of protein energy malnutrition on the capacity of monocytes and macrophages to upregulate arginase, an enzyme associated ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-11-51
更新日期：2014-10-24 00:00:00
abstract：BACKGROUND:We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS). To find biomarkers of calcium deficiency and further confirm these biomarkers in serum, we p...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-020-00507-2
更新日期：2020-11-23 00:00:00
abstract：UNLABELLED: BACKGROUND:Leptin-deficient ob/ob mice exhibit adipocyte hypertrophy and hyperplasia as well as elevated adipose tissue and systemic inflammation. Multipotent stem cells isolated from adult adipose tissue can differentiate into adipocytes ex vivo and thereby contribute toward increased adipocyte cell numbe...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-9-109
更新日期：2012-12-05 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate the relationship between intakes of energy-providing macronutrients, and markers of cardio metabolic risk factors in high BMI African American (AA) children. METHODS:A cross sectional analysis of a sample of 9-11 year old children (n = 80) with BMI greater then the 85th...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-6-41
更新日期：2009-10-13 00:00:00
abstract：BACKGROUND:Fasting is a rigorous type of dietary restriction that is associate with a number of health benefits. During fasting, ketone bodies significantly increase in blood and become major body fuels, thereby sparing glucose. In the present study, we investigated effects of fasting on hypersensitivity. In addition, ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-11-40
更新日期：2014-08-28 00:00:00
abstract：Background:Muscle protein synthesis and muscle net balance plateau after moderate protein ingestion in adults. However, it has been suggested that there is no practical limit to the anabolic response of whole-body net balance to dietary protein. Moreover, limited research has addressed the anabolic response to dietary ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-018-0301-z
更新日期：2018-09-24 00:00:00
abstract：Background:NAFLD as a result of inappropriate diet and obesity, may progress to sever conditions such as: type 2 diabetes mellitus or steatohepatitis, and has recently become a prevalent topic of numerous investigations. Due to its dangerous aftermaths, finding new substances, such as polyphenols and their derivatives,...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-017-0223-1
更新日期：2017-11-13 00:00:00
abstract：PURPOSE:To determine the influence of menstrual irregularity, oral contraceptive use and other factors on bone mineral density (BMD) and bone size at different skeletal sites in 135 college-aged fit women. METHODS:Menstrual history, oral contraceptive use, exercise history, and nutritional factors including calcium, c...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-4-17
更新日期：2007-08-06 00:00:00
abstract：BACKGROUND:Cytokines secreted by adipose tissue macrophages (ATMs) significantly alter adipocyte function, inducing inflammatory responses and decreasing insulin sensitivity. However, little relevant information is available regarding the role of microvesicles (MVs) derived from ATMs in macrophage-adipocyte crosstalk. ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-015-0016-3
更新日期：2015-06-06 00:00:00
abstract：: This paper reviews evidence in the context of current research linking dietary fructose to health risk markers.Fructose intake has recently received considerable media attention, most of which has been negative. The assertion has been that dietary fructose is less satiating and more lipogenic than other sugars. Howev...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-7-82
更新日期：2010-11-04 00:00:00
abstract：BACKGROUND:Recent data from this laboratory suggest that components of dairy foods may serve as activators of SIRT1 (Silent Information Regulator Transcript 1), and thereby participate in regulation of glucose and lipid metabolism. In this study, an ex-vivo/in-vitro approach was used to examine the integrated effects o...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-8-91
更新日期：2011-12-20 00:00:00
abstract：BACKGROUND:Total body water (TBW) estimations have been used to estimate body composition, particularly fat-free mass, to aid in nutritional interventions, and to monitor hydration status. In the past, bioimpedance spectroscopy (BIS) devices have been used to estimate TBW. Previous investigations have examined the vali...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-5-7
更新日期：2008-03-19 00:00:00
abstract：BACKGROUND:Body volume expands with the positive energy balance associated with the development of adult human obesity and this "growth" is captured by two widely used clinical metrics, waist circumference and body mass index (BMI). Empirical correlations between circumferences, BMI, and related body compartments are f...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-5-24
更新日期：2008-10-06 00:00:00
abstract：BACKGROUND:Low plasma high-density lipoprotein-cholesterol (HDL-c) level is commonly present in obesity and represents an independent cardiovascular risk factor. However, obese patients are a very heterogeneous population and the factors and mechanisms that contribute to low HDL-c remain unclear. The aim of this study ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-9-17
更新日期：2012-03-13 00:00:00
abstract：UNLABELLED:Insufficient calcium intake has been proposed to cause unbalanced energy partitioning leading to obesity. However, weight loss interventions including dietary calcium or dairy product consumption have not reported changes in lipid metabolism measured by the plasma lipidome. METHODS:The objective of this stu...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-8-67
更新日期：2011-10-05 00:00:00
abstract：Background:The health benefits of dietary fibre (DF) intake on non-alcoholic fatty liver disease (NAFLD) are controversial. Thus, this large cross-sectional study aimed to determine the associations between DF intake and the prevalence of newly-diagnosed NAFLD in a large general population. Methods:A total of 23,529 p...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-019-0420-1
更新日期：2020-01-13 00:00:00
abstract：Objective:We aimed to describe the 25-hydroxyvitamin D (25(OH)D) status of southern Chinese individuals by a high-accuracy liquid chromatography tandem mass spectrometry (LC-MS/MS) method which can trace to reference measurement procedure. Materials and methods:From January 2018 to June 2019, a total of 4775 southern ...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-020-0427-7
更新日期：2020-01-17 00:00:00
abstract：Background:It is not clear whether the frequently reported phenomenon of exercise-induced anorexia is exacerbated or blunted in warm or cold environments. Therefore, this study investigated the effects of exercise in three different environmental temperatures vs. rest, on perceptions of appetite, appetite regulating ho...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/s12986-019-0348-5
更新日期：2019-05-06 00:00:00
abstract：BACKGROUND:Two major risk factors for the onset of fatty liver disease are excessive alcohol intake and obesity, the latter being associated with non-alcoholic fatty liver disease (NAFLD). The aim of this study was to examine the effects of black rice extract (BRE) on hepatic steatosis and insulin resistance in high-fa...
journal_title：Nutrition & metabolism
pub_type： 杂志文章
doi：10.1186/1743-7075-9-27
更新日期：2012-03-30 00:00:00
