Abstract:
BACKGROUND:A major portion of available fatty acids for adipocyte uptake is derived from lipoprotein lipase (LPL)-mediated hydrolysis of circulating lipoprotein particles. In vivo studies aimed at identifying the precise role of adipocyte-derived LPL in fat storage function of adipose tissue have been unable to provide conclusive evidence due to compensatory mechanisms that activate endogenous fatty acid synthesis. To address this gap in knowledge, we have measured the effect of reducing adipocyte LPL expression on intracellular lipid accumulation using a well-established cultured model of adipocyte differentiation. METHODS:siRNA specific for mouse LPL was transfected into 3T3-L1 adipocytes. Expression of LPL was measured by quantitative real-time PCR and cell surface-associated LPL enzymatic activity was measured by colorimetric detection following substrate (p-nitrophenyl butyrate) hydrolysis. Apolipoprotein CII and CIII expression ratios were also measured by qRT-PCR. Intracellular lipid accumulation was quantified by Nile Red staining. RESULTS:During differentiation of 3T3-L1 pre-adipocytes, LPL mRNA expression increases 6-fold resulting in a 2-fold increase in cell surface-associated LPL enzymatic activity. Parallel to this increase in LPL expression, we found that intracellular lipids increased ~10-fold demonstrating a direct correlation between adipocyte-derived LPL expression and lipid storage. We next reduced LPL expression in adipocytes using siRNA transfections to directly quantify the contributions of adipocyte-derived LPL to lipid storage, This treatment reduced LPL mRNA expression and cell surface-associated LPL enzymatic activity to ~50% of non-treated controls while intracellular lipid levels were reduced by 80%. Exogenous addition of purified LPL (to restore extracellular lipolytic activity) or palmitate (as a source of free fatty acids) to siRNA-treated cells restored intracellular lipid levels to those measured for non-treated controls. We also found that adipocytes express apolipoprotein CII and CIII and, in addition, the apoCII/apoCIII ratio remains largely unchanged in cells with reduced LPL expression. CONCLUSION:We provide evidence that adipocyte-derived LPL is required for efficient fatty acid uptake and storage, and that adipocytes express their own source of apoCII and apoCIII for regulating extracellular LPL activity. These findings demonstrate that adipocytes are capable of producing the necessary enzymatic components and co-factors for efficient lipid storage independent of vascular sources.
journal_name
Nutr Metab (Lond)journal_title
Nutrition & metabolismauthors
Gonzales AM,Orlando RAdoi
10.1186/1743-7075-4-22subject
Has Abstractpub_date
2007-10-30 00:00:00pages
22issn
1743-7075pii
1743-7075-4-22journal_volume
4pub_type
杂志文章abstract:Background:Consumption of diet high in soy products is suggested to contribute to lower prostate cancer incidence in Asian men. But little has been known about the influences of dietary patterns on gut microbiota and microbiota-mediated isoflavone metabolism. Here, we determined the influences of western pattern diet o...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-019-0351-x
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Total body water (TBW) estimations have been used to estimate body composition, particularly fat-free mass, to aid in nutritional interventions, and to monitor hydration status. In the past, bioimpedance spectroscopy (BIS) devices have been used to estimate TBW. Previous investigations have examined the vali...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-5-7
更新日期:2008-03-19 00:00:00
abstract:UNLABELLED: BACKGROUND:It is difficult to study the mechanisms of the metabolic syndrome in humans due to the heterogeneous genetic background and lifestyle. The present study investigated changes in the gene and protein profiles in an animal model of the metabolic syndrome to identify the molecular targets associated...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-9-87
更新日期:2012-09-21 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-11-51
更新日期:2014-10-24 00:00:00
abstract:BACKGROUND:Sub-optimally nourished rats show reduced growth, biochemical and physiological changes. However, no one has assessed metabolic rate adaptations in rats subjected to chronic suboptimal nutrition (CSN). In this study energy expenditure (EE; kcal/100 g body weight) and physical activity (PA; oscillations in we...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-3-11
更新日期:2006-01-31 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-11-57
更新日期:2014-12-23 00:00:00
abstract:Background:It is well known that ingestion of protein sources can stimulate muscle protein synthesis (MPS). The intake of whey protein is highly effective especially for accelerating MPS. Whey protein hydrolysate (WPH) can raise postprandial plasma concentration of amino acids, which impact stimulation of MPS more rapi...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-019-0417-9
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-015-0012-7
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-018-0301-z
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
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更新日期:2016-11-03 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-7-82
更新日期:2010-11-04 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-019-0348-5
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-10-68
更新日期:2013-12-06 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-9-81
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-5-24
更新日期:2008-10-06 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-020-00535-y
更新日期:2021-01-07 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-018-0291-x
更新日期:2018-09-26 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-015-0016-3
更新日期:2015-06-06 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-017-0208-0
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
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更新日期:2020-08-03 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-7-78
更新日期:2010-10-06 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 社论
doi:10.1186/1743-7075-5-10
更新日期:2008-04-09 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-018-0282-y
更新日期:2018-06-22 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-6-13
更新日期:2009-03-24 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-3-38
更新日期:2006-09-12 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/s12986-018-0249-z
更新日期:2018-02-17 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-7-33
更新日期:2010-04-22 00:00:00
abstract:: Impaired kidney function and chronic kidney disease (CKD) leading to kidney failure and end-stage renal disease (ESRD) is a serious medical condition associated with increased morbidity, mortality, and in particular cardiovascular disease (CVD) risk. CKD is associated with multiple physiological and metabolic disturb...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-9-36
更新日期:2012-04-26 00:00:00
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journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-8-30
更新日期:2011-05-12 00:00:00
abstract:BACKGROUND:Recent data from this laboratory suggest that components of dairy foods may serve as activators of SIRT1 (Silent Information Regulator Transcript 1), and thereby participate in regulation of glucose and lipid metabolism. In this study, an ex-vivo/in-vitro approach was used to examine the integrated effects o...
journal_title:Nutrition & metabolism
pub_type: 杂志文章
doi:10.1186/1743-7075-8-91
更新日期:2011-12-20 00:00:00