Abstract:
BACKGROUND:Tissue-transglutaminase (TG2), a dual function G-protein, plays key roles in cell differentiation and migration. In our previous studies we reported the mechanism of TG2-induced cell differentiation. In present study, we explored the mechanism of how TG2 may be involved in cell migration. METHODS:To study the mechanism of TG2-mediated cell migration, we used neuroblastoma cells (SH-SY5Y) which do not express TG2, neuroblastoma cells expressing exogenous TG2 (SHYTG2), and pancreatic cancer cells which express high levels of endogenous TG2. Resveratrol, a natural compound previously shown to inhibit neuroblastoma and pancreatic cancer in the animal models, was utilized to investigate the role of TG2 in cancer cell migration. Immunofluorescence assays were employed to detect expression and intracellular localization of TG2, and calcium levels in the migrating cells. Native gel electrophoresis was performed to analyze resveratrol-induced cellular distribution and conformational states of TG2 in migrating cells. Data are presented as the mean and standard deviation of at least 3 independent experiments. Comparisons were made among groups using one-way ANOVA followed by Tukey-Kramer ad hoc test. RESULTS:TG2 containing cells (SHYTG2 and pancreatic cancer cells) exhibit increased cell migration and invasion in collagen-coated and matrigel-coated transwell plate assays, respectively. Resveratrol (1 μM-10 μM) prevented migration of TG2-expressing cells. During the course of migration, resveratrol increased the immunoreactivity of TG2 without affecting the total TG2 protein level in migrating cells. In these cells, resveratrol increased calcium levels, and depletion of intracellular calcium by a calcium chelator, BAPTA, attenuated resveratrol-enhanced TG2 immunoreactivity. In native-polyacrylamide gels, we detected an additional TG2 protein band with slower migration in total cell lysates of resveratrol treated cells. This TG2 form is non-phosphorylated, exclusively present in plasma membrane fractions and sensitive to intracellular Ca(2+) concentration suggesting a calcium requirement in TG2-regulated cell migration. CONCLUSIONS:Taken together, we conclude that resveratrol induces conformational changes in TG2, and that Ca(2+)-mediated TG2 association with the plasma membrane is responsible for the inhibitory effects of resveratrol on cell migration.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Kumar A,Hu J,LaVoie HA,Walsh KB,DiPette DJ,Singh USdoi
10.1186/1471-2407-14-256subject
Has Abstractpub_date
2014-04-11 00:00:00pages
256issn
1471-2407pii
1471-2407-14-256journal_volume
14pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Bowel cancer is common and is a major cause of death. Most people with bowel symptoms who meet the criteria for urgent referral to secondary care will not be found to have bowel cancer, and some people who are found to have cancer will have been referred routinely rather than urgently. If general practitione...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-6-251
更新日期:2006-10-23 00:00:00
abstract:BACKGROUND:There are large ethnic differences in cervical cancer survival in New Zealand that are only partly explained by stage at diagnosis. We investigated the association of comorbidity with cervical cancer survival, and whether comorbidity accounted for the previously observed ethnic differences in survival. METH...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-132
更新日期:2011-04-12 00:00:00
abstract:BACKGROUND:Patients with advanced or metastatic non-small cell lung cancer (NSCLC) can develop acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (TKIs) erlotinib and gefitinib. Here, we report the successful treatment with alternating chemotherapy and TKIs of two cases of advanced NSCLC...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-90
更新日期:2011-03-02 00:00:00
abstract:BACKGROUND:Breast cancer mortality steadily declined from the 1990s and this has been attributed to early detection and/or to improvements in therapy. Which of those two has had the greater impact is a subject of contention. METHODS:A database of 386 patients, enrolled in a randomized clinical trial on the effect of a...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-702
更新日期:2014-09-24 00:00:00
abstract:BACKGROUND:Thyroid cancer is among the fastest growing malignancies; almost fifty-percent of these rapidly increasing incidence tumors are less than or equal to 1cm in size, termed papillary thyroid microcarcinoma (PTMC). The management of PTMC remains a controversy due to differing natural history of these patients. E...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-523
更新日期:2012-11-15 00:00:00
abstract:BACKGROUND:Despite all benefit provided by established therapies prognosis of gastric cancer remains poor. Targeted inhibition of platelet derived growth factor receptor (PDGFR) by imatinib may influence tumor growth and amplify chemotherapeutic effects. METHODS:This phase I study evaluated dose limiting toxicity (DLT...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-12-587
更新日期:2012-12-10 00:00:00
abstract:BACKGROUND:Lymphocyte Function-Associated Antigen-1 (LFA-1; CD18/CD11a) is one of the main adhesion molecules used by immune cells to infiltrate the liver under inflammatory conditions. Recently, the expression of this integrin has also been reported on several solid tumors, including colorectal cancer. However, its fu...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3823-2
更新日期:2017-12-06 00:00:00
abstract:BACKGROUND:Early diagnosis is important for the timely treatment of gallbladder carcinoma (GBC) patients and may lead to increased survival outcomes. Here, we have applied serological proteome analysis (SERPA), an immunoproteomics approach, for the detection of 'tumor-associated antigens (TAAs) that elicit humoral resp...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07676-6
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:To investigate the implications of prophylactic intraoperative Hyperthermic Intraperitoneal Chemotherapy (HIPEC) with D2 radical gastrectomy for locally advanced Gastric Cancer (AGC) in a randomized case control study. METHOD:Eighty consecutive patients with locally AGC were randomly separated into 2 groups...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6125-z
更新日期:2019-09-18 00:00:00
abstract:BACKGROUND:The sex hormone-binding globulin (SHBG) is a carrier protein that modulates the bio-availability of serum sex steroid hormones, which may be involved in ovarian cancer. We evaluated whether common genetic variation in SHBG and its 3' neighbor ATP1B2, in linkage disequilibrium, is associated with the risk of ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-7-60
更新日期:2007-04-05 00:00:00
abstract:BACKGROUND:ZFP36 is an mRNA binding protein that exerts anti-tumor activity in glioblastoma by triggering cell death, associated to an increase in the stability of the kinase RIP1. METHODS:We used cell death assays, size exclusion chromatography, Co-Immunoprecipitation, shRNA lentivectors and glioma neural stem cells ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1388-5
更新日期:2015-05-06 00:00:00
abstract:BACKGROUND:The first-generation epidermal growth factor receptor tyrosine kinase inhibitors gefitinib and erlotinib have both been proven effective for treating advanced non-small cell lung cancer (NSCLC), especially in East Asian patients. We conducted this meta-analysis to compare their efficacy and safety in treatin...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析
doi:10.1186/s12885-018-4685-y
更新日期:2018-08-02 00:00:00
abstract:BACKGROUND:Photodynamic therapy (PDT) of solid cancers comprises the administration of a photosensitizer followed by illumination of the photosensitizer-replete tumor with laser light. This induces a state of local oxidative stress, culminating in the destruction of tumor tissue and microvasculature and induction of an...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1994-2
更新日期:2015-12-26 00:00:00
abstract:BACKGROUND:The incidence of papillary thyroid cancer (PTC) is markedly higher in women than men during the reproductive years. In vitro studies have suggested that estrogen may play an important role in the development and progression of PTC through estrogen receptors (ERs). This study aimed to investigate the expressi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-383
更新日期:2014-05-29 00:00:00
abstract:BACKGROUND:Analysis of the clinical response of extramedullary lesions in leukemic patients treated with radiation therapy (RT) and defining the optimal dose of radiation. METHODS:Forty-two extramedullary lesions found in 24 leukemic patients treated with RT were reviewed. The radiation was delivered usually 2 Gy/day,...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-428
更新日期:2011-10-06 00:00:00
abstract:BACKGROUND:The human leucine-rich, repeat-containing G protein-coupled receptor 5 (LGR5) is a stem cell marker in numerous adult tissues and is overexpressed in a large number of human carcinoma including colon cancer, breast cancer and oral squamous cell carcinomas (OSCC). The role of the full length transcript (LGR5F...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5327-8
更新日期:2019-02-15 00:00:00
abstract:BACKGROUND:It has been previously demonstrated in several cancer models, that Dronabinol (THC) may have anti-tumor activity--however, controversial data exists for acute leukemia. We have anecdotal evidence that THC may have contributed to disease control in a patient with acute undifferentiated leukemia. METHODS:To t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-2029-8
更新日期:2016-01-16 00:00:00
abstract:BACKGROUND:Acute lymphoblastic leukemia (ALL) is a heterogeneous group of malignant disorders derived from B- or T-cell lymphoid progenitor cells. ALL often is refractory to or relapses after treatment; thus, novel targeted therapy for ALL is urgently needed. In the present study, we initially found that the level of S...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1282-1
更新日期:2015-04-07 00:00:00
abstract:BACKGROUND:RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients. METHODS:Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-22
更新日期:2012-01-17 00:00:00
abstract:BACKGROUND:Colon cancer is the most common type of gastrointestinal cancer and has high morbidity and mortality. Colon adenocarcinoma (COAD) is the main pathological type of colon cancer, and much evidence has supported the correlation between the prognosis of COAD and the immune system. The current study aimed to deve...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07532-7
更新日期:2020-11-09 00:00:00
abstract:BACKGROUND:Less than 20% of familial breast cancer patients who undergo genetic testing for BRCA1 and BRCA2 carry a pathogenic mutation in one of these two genes. The GENESIS (GENE SISter) study was designed to identify new breast cancer susceptibility genes in women attending cancer genetics clinics and with no BRCA1/...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-2028-9
更新日期:2016-01-12 00:00:00
abstract:BACKGROUND:A number of osteoblastic meningiomas, primary osteosarcomas of the meninges, and post-radiation osteosarcomas of the head have been reported. However, postradiation dedifferentiation of meningioma into osteosarcoma has not been reported previously. CASE PRESENTATION:In 1987 a caucasian man, then 38 years ol...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-2-34
更新日期:2002-12-03 00:00:00
abstract:BACKGROUND:To evaluate efficacy in patients with brain metastasis (BM) on entry into the lapatinib expanded access program (LEAP). METHODS:LEAP is a worldwide, single-arm, open-label study. HER2-positive, locally-advanced or metastatic breast cancer patients with progression after an anthracycline, taxane, and trastuz...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/1471-2407-12-322
更新日期:2012-07-28 00:00:00
abstract:BACKGROUND:Gastric cancer peritoneal carcinomatosis is a common clinical problem, but there are no suitable large animal models to study this problem. This study was to establish a stable rabbit peritoneal carcinomatosis model of gastric cancer using VX2 tumor, and analyze the clinico-pathological features. METHODS:VX...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-124
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:Breast cancer can impact survivors in many aspects of their life. Scarce information is currently available on the quality of life of cancer survivors in Bahrain. The objective of this study is to describe the quality of life of Bahraini women with breast cancer and its association with their sociodemographi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-212
更新日期:2013-04-28 00:00:00
abstract:BACKGROUND:Based on the mechanism of action, combining somatostatin analogues (SSAs) with mTOR inhibitors or antiangiogenic agents may provide synergistic effects for the treatment of patients with neuroendocrine tumours (NETs). Herein, we investigate the use of these treatment combinations in clinical practice. METHO...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1512-6
更新日期:2015-07-04 00:00:00
abstract:BACKGROUND:Inducible nitric oxide synthase (iNOS) metabolizes L-arginine to produce nitric oxide (NO) which was originally identified in myeloid cells as a host defense mechanism against pathogens. Recent studies, however, have revealed that iNOS is often induced in tumor cells and myeloid cells in the tumor microenvir...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1808-6
更新日期:2015-10-23 00:00:00
abstract:BACKGROUND:Pancreatic cancer remains the deadliest of all cancers, with a mortality rate of 91%. Gemcitabine is considered the gold chemotherapeutic standard, but only marginally improves life-span due to its chemical instability and low cell penetrance. A new paradigm to improve Gemcitabine's therapeutic index is to a...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-419
更新日期:2012-09-22 00:00:00
abstract:BACKGROUND:Albendazole (ABZ) is a microtubule-targeting anthelmintic with a remarkable activity against a variety of human cancer cells. In this study, we examined if the antitumor activity of ABZ could be enhanced by its combination with other microtubule-binding agents. METHODS:The interactions between ABZ and micro...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-86
更新日期:2013-02-23 00:00:00
abstract:BACKGROUND:Patients suffering from squamous cell carcinoma of the larynx (LSCC) with lymphatic metastasis have a relatively poor prognosis and often require radical therapeutic management. The mechanisms which drive metastasis to the lymph nodes are largely unknown but may be promoted by a pro-angiogenic tumor microenv...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4180-5
更新日期:2018-03-09 00:00:00