Abstract:
BACKGROUND:Rapid diagnostic tests (RDTs) for malaria provide a practical alternative to light microscopy for malaria diagnosis in resource-limited settings. Three-band RDTs incorporating two parasite antigens may have enhanced diagnostic specificity, relative to two-band RDTs with a single parasite antigen (typically histidine-rich protein 2 [HRP2]). METHODS:Phase 1: 2,000 children, two months to five years of age, admitted to a referral hospital in Jinja, Uganda, with acute febrile illness were enrolled. A WHO highly rated three-band RDT was compared to light microscopy of thick peripheral blood films read by local expert microscopists.Phase 2: the three-band RDT was used as a screening tool for inclusion of patients in a clinical trial, and subjects with three positive RDT bands were tested by microscopy using blood samples drawn in parallel. Discordant results were adjudicated by PCR. RESULTS:Phase 1: 1,648 children had both a RDT and peripheral blood smear performed. The specificity of a RDT with all three bands positive was 82% (95% CI: 79-85%) compared to 62% (95% CI: 59-66%) for HRP2 alone. The sensitivity was 88% (95% CI: 85-89%) and 94% (95% CI: 92-95%) for three-band positive RDT and HRP2 antigen, respectively. 119 patients (7.2%) had a positive HRP2 band, but negative parasite lactate dehydrogenase (pLHD) band and negative peripheral smear, and 72 (61%) of these had received pre-treatment with anti-malarials, suggesting a false positive HRP2 result (p = 0.002).Phase 2: the positive predictive value (PPV) of the three-band RDT was 94% (95% CI 89%-97%) using microscopy as the reference standard. However, microscopy-discordant results were shown to be positive for P. falciparum by PCR in all cases, suggesting that the PPV was in fact higher. CONCLUSION:The pLDH antigen on three-band RDTs, used in combination with HRP2, provides added diagnostic specificity for malaria parasitaemia and may be useful to distinguish acute infection from recently treated infection. In situations where diagnostic specificity is desirable (e.g., for selection of malaria-infected participants in clinical trials), a three-band RDT should be considered in a sub-Saharan African setting.
journal_name
Malar Jjournal_title
Malaria journalauthors
Hawkes M,Conroy AL,Opoka RO,Namasopo S,Liles WC,John CC,Kain KCdoi
10.1186/1475-2875-13-43subject
Has Abstractpub_date
2014-02-01 00:00:00pages
43issn
1475-2875pii
1475-2875-13-43journal_volume
13pub_type
杂志文章,随机对照试验相关文献
MALARIA JOURNAL文献大全abstract::Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of Plasmodium falciparum. Compared to standard protocols plate-based t...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-22
更新日期:2012-01-18 00:00:00
abstract:BACKGROUND:Polymorphism in the pfcrt gene underlies Plasmodium falciparum chloroquine resistance (CQR), as sensitive strains consistently carry lysine (K), while CQR strains carry threonine (T) at the codon 76. Previous studies have shown that microsatellite (MS) haplotype variation can be used to study the evolution o...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-6-34
更新日期:2007-03-21 00:00:00
abstract:BACKGROUND:Rapid diagnostic tests (RDT) and real-time PCR (qPCR) assays are sensitive for diagnosing malaria, but because they detect antigen and DNA, respectively, positivity may not reflect active infection. Performance characteristics of RDT and qPCR in Plasmodium falciparum positive specimens were evaluated over ti...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0882-1
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:Most rapid diagnostic tests (RDTs) currently used for malaria diagnosis cannot distinguish the various Plasmodium infections. The development of a Plasmodium vivax specific RDTs with high sensitivity to sufficiently differentiate the two most common Plasmodium infections would be very crucial for disease tre...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-199
更新日期:2013-06-12 00:00:00
abstract:BACKGROUND:The role of the liver for survival of blood-stage malaria is only poorly understood. In experimental blood-stage malaria with Plasmodium chabaudi, protective vaccination induces healing and, thus, survival of otherwise lethal infections. This model is appropriate to study the role of the liver in vaccination...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2366-6
更新日期:2018-05-29 00:00:00
abstract:BACKGROUND:Malaria remains a major public health problem, and its control has been hampered by drug resistance. For a number of drugs, Plasmodium falciparum single nucleotide polymorphisms (SNPs) are associated with altered drug sensitivity and can be used as markers of drug resistance. Several techniques have been stu...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-95
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:Deltamethrin-impregnated, long-lasting insecticidal nets (LLINs) were distributed in the study area from November 2014 to January 2015 to evaluate their impact on malaria transmission in the presence of insecticide-resistant vectors. Studies were carried out in 16 selected clusters in Keshkal sub-district, C...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2557-1
更新日期:2018-11-08 00:00:00
abstract:BACKGROUND:The receptors for adhesion of Plasmodium falciparum-infected red blood cells (RBC) in the placenta have been identified as chondroitin-4-sulphate (C4S) proteoglycans, and the more sulphate-rich chondroitin oligosaccharides have been reported to inhibit adhesion. Since the anti-malarial drug chloroquine accum...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-303
更新日期:2009-12-17 00:00:00
abstract:BACKGROUND:The recent reports on the decreasing susceptibility of Plasmodium falciparum to artemisinin derivatives along the Thailand and Myanmar border are worrying. Indeed it may spread to India and then Africa, repeating the same pattern observed for chloroquine resistance. Therefore, it is essential to start monito...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-207
更新日期:2014-05-31 00:00:00
abstract:BACKGROUND:Members of the Anopheles punctulatus group (AP group) are the primary vectors of human malaria in Papua New Guinea. The AP group includes 13 sibling species, most of them morphologically indistinguishable. Understanding why only certain species are able to transmit malaria requires a better comprehension of ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-64
更新日期:2013-02-14 00:00:00
abstract::BACKGROUND: Removal of exhaled air from total body emanations or artificially standardising carbon dioxide (CO2) outputs has previously been shown to eliminate differential attractiveness of humans to certain blackfly (Simuliidae) and mosquito (Culicidae) species. Whether or not breath contributes to between-person di...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-3-1
更新日期:2004-01-29 00:00:00
abstract:BACKGROUND:Plasmodium vivax parasites are the predominant cause of malaria infections in the Brazilian Amazon. Infected individuals are treated with primaquine, which can induce haemolytic anaemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals and may lead to severe and fatal complications. This X-li...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1889-6
更新日期:2017-06-15 00:00:00
abstract:BACKGROUND:The emergence and spread of Plasmodium falciparum resistance to artemisinin-based combination therapy in Southeast Asia prompted the need to develop new endoperoxide-type drugs. METHODS:A chemically diverse library of endoperoxides was designed and synthesized. The compounds were screened for in vitro and i...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2281-x
更新日期:2018-04-03 00:00:00
abstract:BACKGROUND:During malaria infection the Toll-like receptor 9 (TLR9) is activated through induction with plasmodium DNA or another malaria motif not yet identified. Although TLR9 activation by malaria parasites is well reported, the implication to the susceptibility to severe malaria is not clear. The aim of this study ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-44
更新日期:2009-03-13 00:00:00
abstract:BACKGROUND:The present study evaluated the SD Bioline Malaria Ag 05FK40 (SDFK40), a three-band RDT detecting Plasmodium falciparum-specific parasite lactate dehydrogenase (Pf-pLDH) and pan Plasmodium-specific pLDH (pan-pLDH), in a reference setting. METHODS:The SDFK40 was retrospectively and prospectively tested again...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-7
更新日期:2011-01-12 00:00:00
abstract:BACKGROUND:Primaquine is an anti-malarial used to prevent Plasmodium vivax relapses and malaria transmission. However, PQ metabolites cause haemolysis in patients deficient in the enzyme glucose-6-phosphate dehydrogenase (G6PD). Fifteen PQ-thiazolidinone derivatives, synthesized through one-post reactions from primaqui...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1755-6
更新日期:2017-03-09 00:00:00
abstract:BACKGROUND:Malaria is one of the most severe public health issues that result in massive morbidity and mortality in most countries of the sub-Saharan Africa (SSA). This study aimed to determine the scope of household, accessibility to malaria care and factors associated with household malaria in the Democratic Republic...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2679-0
更新日期:2019-02-26 00:00:00
abstract:BACKGROUND:Kenya recently changed its antimalarial drug policy to a specific artemisinin-based combination therapy (ACT), artemether-lumefantrine (AL). New national guidelines on the diagnosis, treatment and prevention were developed and disseminated to health workers together with in-service training. METHODS:Between...
journal_title:Malaria journal
pub_type: 杂志文章,多中心研究
doi:10.1186/1475-2875-7-29
更新日期:2008-02-05 00:00:00
abstract:BACKGROUND:Molecular tools are very sensitive and specific and could be an alternative for the diagnosis of malaria. The complexity and need for expensive equipment may hamper implementation and, therefore, simplifications to current protocols are warranted. METHODS:A PCR detecting the different Plasmodium species and...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-279
更新日期:2012-08-17 00:00:00
abstract:BACKGROUND:Rapid elimination of Plasmodium falciparum malaria in Cambodia is a goal with both national and international significance. Transmission of malaria in Cambodia is limited to forest environments, and the main population at risk consists of forest-goers who rely on forest products for income or sustenance. The...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03572-3
更新日期:2021-01-20 00:00:00
abstract:BACKGROUND:Obtaining single parasite clones is required for many techniques in malaria research. Cloning by limiting dilution using microscopy-based assessment for parasite growth is an arduous and labor-intensive process. An alternative method for the detection of parasite growth in limiting dilution assays is using a...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-95
更新日期:2011-04-18 00:00:00
abstract::This opinion article deals with the diagnostic clinical challenges faced by clinicians or health care workers in malaria-endemic areas when a severely sick child presents to the clinic with fever, coma or respiratory distress. Indeed, the coexistence of malaria with other severe infections like meningitis, invasive ba...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2533-9
更新日期:2018-10-22 00:00:00
abstract:BACKGROUND:The objective of this project was to achieve high, sustainable levels of net coverage in a village in rural Tanzania by combining free distribution of long-lasting insecticide-impregnated nets (LLINs) with community-tailored education. In Tanzania, malaria is the leading cause of morbidity and mortality. Alt...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-315
更新日期:2009-12-31 00:00:00
abstract:BACKGROUND:Early diagnosis and prompt treatment including appropriate home-based treatment of malaria is a major strategy for malaria control. A major determinant of clinical outcome in case management is compliance and adherence to effective antimalarial regimen. Home-based malaria treatment with inappropriate medicin...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-143
更新日期:2008-07-29 00:00:00
abstract:BACKGROUND:The Kilombero Valley is a highly malaria-endemic agricultural area in south-eastern Tanzania. Seasonal flooding of the valley is favourable to malaria transmission. During the farming season, many households move to distant field sites (shamba in Swahili) in the fertile river floodplain for the cultivation o...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-7
更新日期:2008-01-09 00:00:00
abstract:BACKGROUND:In many low-income countries, the private commercial sector plays an important role in the provision of malaria treatment. However, the quality of care it provides is often poor, with artemisinin combination therapy (ACT) generally being too costly for consumers. Decreasing ACT prices is critical for improvi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0737-9
更新日期:2015-05-30 00:00:00
abstract:BACKGROUND:Premature death of Plasmodium-infected erythrocytes is considered to favourably influence the clinical course of malaria. Aurothiomalate has previously been shown to trigger erythrocyte death or eryptosis, which is characterized by cell membrane scrambling leading to phosphatidylserine exposure at the cell s...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-118
更新日期:2010-05-07 00:00:00
abstract:BACKGROUND:In a malaria-endemic area the distribution of patients is neither constant in time nor homogeneous in space. The WHO recommends the stratification of malaria risk on a fine geographical scale. In the village of Cacao in French Guiana, the study of the spatial and temporal distribution of malaria cases, durin...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-90
更新日期:2013-03-11 00:00:00
abstract:BACKGROUND:Malaria caused by Plasmodium falciparum in pregnancy can result in adverse maternal and fetal sequelae. This review evaluated the adherence of the national guidelines drawn from World Health Organization (WHO) regions, Africa, Eastern Mediterranean, Southeast Asia, and Western Pacific, to the WHO recommendat...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/s12936-020-03565-2
更新日期:2021-01-23 00:00:00
abstract::Artesunate (AS) is a clinically versatile artemisinin derivative utilized for the treatment of mild to severe malaria infection. Given the therapeutic significance of AS and the necessity of appropriate AS dosing, substantial research has been performed investigating the pharmacokinetics of AS and its active metabolit...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-10-263
更新日期:2011-09-13 00:00:00