Abstract:
BACKGROUND:Molecular tools are very sensitive and specific and could be an alternative for the diagnosis of malaria. The complexity and need for expensive equipment may hamper implementation and, therefore, simplifications to current protocols are warranted. METHODS:A PCR detecting the different Plasmodium species and differentiating between Plasmodium falciparum and Plasmodium vivax was developed and combined with a nucleic acid lateral flow immuno-assay (PCR-NALFIA) for amplicon detection. The assay was thoroughly evaluated for the analytical sensitivity and specificity in the laboratory, the robustness and reproducibility in a ring trial and accuracy and predictive value in a field trial. RESULTS:The analytical sensitivity and specificity were 0.978 (95% CI: 0.932-0.994) and 0.980 (95% CI: 0.924-0.997), respectively, and were slightly less sensitive for the detection of P. vivax than for P. falciparum. The reproducibility tested in three laboratories was very good (k = 0.83). This evaluation showed that the PCR machine used could influence the results. Accuracy was evaluated in Thailand and compared to expert microscopy and rapid diagnostic tests (RDTs). The overall and P. falciparum-specific sensitivity and specificity was good ranging from 0.86-1 and 0.95-0.98 respectively, compared to microscopy. Plasmodium vivax detection was better than the sensitivity of RDT, but slightly less than microscopy performed in this study. CONCLUSION:PCR-NALFIA is a sensitive, specific and robust assay able to identify Plasmodium species with good accuracy. Extensive testing including a ring trial can identify possible bottlenecks before implementation and is therefore essential to perform in additon to other evaluations.
journal_name
Malar Jjournal_title
Malaria journalauthors
Mens PF,Moers AP,de Bes LM,Flint J,Sak JR,Keereecharoen L,van Overmeir C,Verweij JJ,Hallett RL,Wihokhoen B,Proux S,Schallig HD,van Amerongen Adoi
10.1186/1475-2875-11-279subject
Has Abstractpub_date
2012-08-17 00:00:00pages
279issn
1475-2875pii
1475-2875-11-279journal_volume
11pub_type
杂志文章相关文献
MALARIA JOURNAL文献大全abstract:BACKGROUND:In Uganda, treatment of clinical malaria and intermittent preventive treatment with sulphadoxine-pyrimethamine (SP) are common during pregnancy. As a result, both formal and informal reports from antenatal sources suggest possible misuse of SP for malaria treatment among pregnant women. The objective of this...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0702-7
更新日期:2015-05-03 00:00:00
abstract:BACKGROUND:The number of clinical cases of malaria is often recorded in resource constrained or conflict settings as a proxy for disease burden. Interpreting case count data in areas of humanitarian need is challenging due to uncertainties in population size caused by security concerns, resource constraints and populat...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2460-9
更新日期:2018-08-29 00:00:00
abstract:BACKGROUND:Plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a characteristic set of mutations in the dihydrofolate reductase (PfDHFR) gene that leads to reduced competitive drug binding at the enzyme's active site. Analogous mutations can be found in the DHFR gene in...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-65
更新日期:2010-03-02 00:00:00
abstract:BACKGROUND:Colombia contributes a significant proportion of malaria cases in the Americas, which are predominantly rural. However, in the last 8 years ~ 10 % of the endemic municipalities have also reported urban and peri-urban malaria cases, a growing concern for health authorities. This study focused on the character...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0956-0
更新日期:2015-11-14 00:00:00
abstract::The classic formulae in malaria epidemiology are reviewed that relate entomological parameters to malaria transmission, including mosquito survivorship and age-at-infection, the stability index (S), the human blood index (HBI), proportion of infected mosquitoes, the sporozoite rate, the entomological inoculation rate ...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-3-13
更新日期:2004-06-04 00:00:00
abstract:BACKGROUND:The resistance of malaria parasites to sulphadoxine-pyrimethamine (SP) in 2007 led to the Malawi Ministry of Health changing to artemether-lumefantrine (AL) as first-line for uncomplicated malaria treatment. This study determined the efficacy and safety of AL for the treatment of uncomplicated Plasmodium fal...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0701-8
更新日期:2015-04-23 00:00:00
abstract:BACKGROUND:Mosquito-feeding assays are important tools to guide the development and support the evaluation of transmission-blocking interventions. These functional bioassays measure the sporogonic development of gametocytes in blood-fed mosquitoes. Measuring the infectivity of low gametocyte densities has become increa...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1959-9
更新日期:2017-08-05 00:00:00
abstract:BACKGROUND:Indonesia is home to a variety of malaria vectors whose specific bionomic traits remain largely uncharacterized. Species-specific behaviours, such as host feeding preferences, impact the dynamics of malaria transmission and the effectiveness of vector control interventions. METHODS:To examine species-specif...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1950-5
更新日期:2017-08-02 00:00:00
abstract::Malaria currently causes more harm to human beings than any other parasitic disease, and disproportionally affects low-income populations. The ethical issues raised by efforts to control or eliminate malaria have received little explicit analysis, in comparison with other major diseases of poverty. While some ethical ...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/s12936-015-1042-3
更新日期:2015-12-22 00:00:00
abstract:BACKGROUND:In view of the close relationship of Portugal with African countries, particularly former Portuguese colonies, the diagnosis of malaria is not a rare thing. When a traveller returns ill from endemic areas, malaria should be the number one suspect. World Health Organization treatment guidelines recommend that...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-96
更新日期:2012-03-29 00:00:00
abstract:BACKGROUND:Adequate malaria diagnosis and treatment remain major difficulties in rural sub-Saharan Africa. These issues deserve renewed attention in the light of first-line treatment with expensive artemisinin-combination therapy (ACT) and changing patterns of transmission intensity. This study describes diagnostic and...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-232
更新日期:2008-11-05 00:00:00
abstract:BACKGROUND:Helminths and malaria are among the most prevalent infectious diseases in the world. They both occur in tropical area where they often affect the same populations. There are studies suggesting an effect of helminths on malariometric indices. For example, malaria attacks as well as disease severity has been s...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0608-4
更新日期:2015-02-25 00:00:00
abstract:BACKGROUND:Kohat district is one of the medium intensity malaria transmission areas in Pakistan where asymptomatic carriers are likely to form a reservoir of infection. This study was done to explore the possibility of using microscopy, rapid diagnostic testing (RDT), real time polymerase chain reaction (RT-PCR) and RT...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2191-y
更新日期:2018-01-22 00:00:00
abstract:BACKGROUND:Global efforts to address the burden of malaria have stagnated in recent years with malaria cases beginning to rise. Substandard and falsified anti-malarial treatments contribute to this stagnation. Poor quality anti-malarials directly affect health outcomes by increasing malaria morbidity and mortality, as ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2628-3
更新日期:2019-01-09 00:00:00
abstract:BACKGROUND:Over the last decade, the incidence of confirmed malaria has declined significantly in Nepal. The aim of this paper is to assess the spatio-temporal distribution of malaria and its association with climatic factors and vector control interventions in two high-risk districts of Nepal. METHODS:Hotspot analysi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-457
更新日期:2014-11-25 00:00:00
abstract:BACKGROUND:Determination of the genetic diversity of malaria parasites can inform the intensity of transmission and identify potential deficiencies in malaria control programmes. This study was conducted to characterize the genetic diversity and allele frequencies of Plasmodium falciparum in Northwest Ethiopia along th...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2540-x
更新日期:2018-10-25 00:00:00
abstract::While significant advances have been made in the prevention and treatment of malaria in recent years, these successes continue to fall short of the World Health Organization (WHO) goals for malaria control and elimination. For elimination strategies to be effective, limited disease transmission, achieved through rapid...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-13-291
更新日期:2014-07-28 00:00:00
abstract::The splenic complications of acute malaria include two different prognostic and treatment entities: splenic infarction and splenic rupture. This is the first case of splenic infarction during an acute malaria due to Plasmodium ovale in a 34-year-old man. As in the majority other described cases of splenic infarction, ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-288
更新日期:2010-10-18 00:00:00
abstract:BACKGROUND:Major investments have been made since 2001, with intensification of malaria control interventions after 2006. Interventions included free distribution of insecticide-treated nets (ITN) to pregnant women and children under 5 years old, the introduction of artemisinin combination therapy (ACT) for malaria tre...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2573-1
更新日期:2018-11-14 00:00:00
abstract:BACKGROUND:Artemisinin-derivative formulations are now widely used to treat falciparum malaria. However, the dry powder suspensions developed for children are few and/or are of poor quality. In addition to the active compound, the presence of a suitable preservative in these medicines is essential. In this study, an ev...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-6-12
更新日期:2007-01-26 00:00:00
abstract:BACKGROUND:In areas with ongoing malaria transmission, strategies to clear parasites from populations can reduce infection and transmission. The objective of this paper was to describe a malaria mass testing and treatment (MTAT) intervention implemented in six kebeles (villages) in Amhara Region, Ethiopia, at the begin...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1333-3
更新日期:2016-06-02 00:00:00
abstract:BACKGROUND:In the last decade, several epidemiological studies have demonstrated the potential of using seroprevalence (SP) and seroconversion rate (SCR) as informative indicators of malaria burden in low transmission settings or in populations on the cusp of elimination. However, most of studies are designed to contro...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0661-z
更新日期:2015-04-03 00:00:00
abstract:BACKGROUND:The accuracy of the World Health Organization method of estimating malaria parasite density from thick blood smears by assuming a white blood cell (WBC) count of 8,000/μL has been questioned in several studies. Since epidemiological investigations, anti-malarial efficacy trials and routine laboratory reporti...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-145
更新日期:2014-04-16 00:00:00
abstract:BACKGROUND:There is growing concern that malaria vector resistance to pyrethroid insecticides may reduce the effectiveness of long-lasting insecticidal nets (LLINs). Combination LLINs are designed to control susceptible and pyrethroid-resistant mosquito populations through a mixture of pyrethroid with piperonyl butoxid...
journal_title:Malaria journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12936-017-2124-1
更新日期:2017-11-21 00:00:00
abstract::The outcomes from recent high profile deliberations concerning African health research and economic development all point towards the need for a mechanism to support health innovation on the continent. The mission of the African Network for Drugs and Diagnostics Innovation (ANDI), is to promote and sustain African-led...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-10-S1-S9
更新日期:2011-03-15 00:00:00
abstract:BACKGROUND:Chloroquine has been the treatment of choice for acute vivax malaria for more than 60 years. Malaria caused by Plasmodium vivax has recently shown resistance to chloroquine in some places. This study compared the efficacy and safety of fixed dose combination (FDC) of arterolane maleate and piperaquine phosph...
journal_title:Malaria journal
pub_type: 杂志文章,多中心研究
doi:10.1186/s12936-016-1084-1
更新日期:2016-01-27 00:00:00
abstract:BACKGROUND:The successful control of insect disease vectors relies on a thorough understanding of their ecology and behaviour. However, knowledge of the ecology of many human disease vectors lags behind that of agricultural pests. This is partially due to the paucity of experimental tools for investigating their ecolog...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-356
更新日期:2010-12-08 00:00:00
abstract:BACKGROUND:Malaria during pregnancy is associated with poor maternal and pregnancy outcome and the World Health Organization recommends the administration of intermittent preventive treatment in pregnancy (IPTp) with sulfadoxine-pyrimethamine (SP) and distribution of insecticide-treated mosquito nets (ITNs) to all preg...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1872-2
更新日期:2017-05-25 00:00:00
abstract:BACKGROUND:In malaria-endemic areas, human populations are frequently exposed to immunomodulatory salivary components injected during mosquito blood feeding. The consequences on pathogen-specific immune responses are not well known. This study evaluated and compared the humoral responses specific to merozoite stage vac...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03160-5
更新日期:2020-02-21 00:00:00
abstract:BACKGROUND:Prompt, quality assured laboratory diagnosis is key to effective malaria case management and control, especially since the introduction of the more expensive artemisinin combination therapy (ACT). The malaria programme and its non-government partners, on the basis of WHO recommended Lot Quality Assurance met...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-45
更新日期:2011-02-17 00:00:00