An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites.

abstract：BACKGROUND:The erythrocytic stage, where malaria parasites proliferate in human blood, is clinically significant as this causes the symptoms and illness of malaria. Experimental rodent models of malaria at the erythrocytic stage are used for the development of anti-malarial drugs and for biological analysis. An automat...

journal_title：Malaria journal

authors： Tougan T,Suzuki Y,Izuka M,Aono K,Okazaki T,Toya Y,Uchihashi K,Horii T

更新日期：2018-04-16 00:00:00

abstract：BACKGROUND:Molecular tools for detection of low-density asymptomatic Plasmodium infections are needed in malaria elimination efforts. This study reports results from the hitherto largest implementation of loop-mediated isothermal amplification (LAMP) for centralized mass screening of asymptomatic malaria in Zanzibar. ...

journal_title：Malaria journal

authors： Morris U,Khamis M,Aydin-Schmidt B,Abass AK,Msellem MI,Nassor MH,González IJ,Mårtensson A,Ali AS,Björkman A,Cook J

更新日期：2015-05-17 00:00:00

abstract：BACKGROUND:Plasmodium falciparum merozoite antigens elicit antibody responses in malaria-endemic populations, some of which are clinically protective, which is one of the reasons why merozoite antigens are the focus of malaria vaccine development efforts. Polymorphisms in several merozoite antigen-encoding genes are th...

journal_title：Malaria journal

authors： Ochola-Oyier LI,Okombo J,Wagatua N,Ochieng J,Tetteh KK,Fegan G,Bejon P,Marsh K

更新日期：2016-05-06 00:00:00

abstract：BACKGROUND:Laboratory studies have demonstrated that a variety of immune signaling pathways regulate malaria parasite infection in Anopheles gambiae, the primary vector species in Africa. METHODS:To begin to understand the importance of these associations under natural conditions, an association mapping approach was a...

journal_title：Malaria journal

authors： Horton AA,Lee Y,Coulibaly CA,Rashbrook VK,Cornel AJ,Lanzaro GC,Luckhart S

更新日期：2010-06-11 00:00:00

abstract：:Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of Plasmodium falciparum. Compared to standard protocols plate-based t...

journal_title：Malaria journal

authors： Caro F,Miller MG,DeRisi JL

更新日期：2012-01-18 00:00:00

abstract：BACKGROUND:Molecular detection of low-density Plasmodium falciparum infections is essential for surveillance studies conducted to inform malaria control strategies in close-to-elimination settings. Molecular monitoring of residual malaria infections usually requires a large study size, therefore sampling and diagnostic...

journal_title：Malaria journal

authors： Grossenbacher B,Holzschuh A,Hofmann NE,Omar KA,Stuck L,Fakih BS,Ali A,Yukich J,Hetzel MW,Felger I

更新日期：2020-01-29 00:00:00

abstract：BACKGROUND:Polymorphism in the pfcrt gene underlies Plasmodium falciparum chloroquine resistance (CQR), as sensitive strains consistently carry lysine (K), while CQR strains carry threonine (T) at the codon 76. Previous studies have shown that microsatellite (MS) haplotype variation can be used to study the evolution o...

journal_title：Malaria journal

authors： DaRe JT,Mehlotra RK,Michon P,Mueller I,Reeder J,Sharma YD,Stoneking M,Zimmerman PA

更新日期：2007-03-21 00:00:00

abstract：BACKGROUND:Thrombocytopaenia is the most frequent malaria-associated haematologic alteration observed with all five Plasmodium parasites causing disease in humans. Although not included in the World Health Organization criteria for severe Plasmodium falciparum malaria, severe thrombocytopaenia has been increasingly men...

journal_title：Malaria journal

authors： Antinori S,Corona A,Ridolfo AL,Galimberti L,Ricaboni D,Milazzo L,Corbellino M

更新日期：2016-02-19 00:00:00

abstract：BACKGROUND:Over the last decade, the incidence of confirmed malaria has declined significantly in Nepal. The aim of this paper is to assess the spatio-temporal distribution of malaria and its association with climatic factors and vector control interventions in two high-risk districts of Nepal. METHODS:Hotspot analysi...

journal_title：Malaria journal

authors： Dhimal M,O'Hara RB,Karki R,Thakur GD,Kuch U,Ahrens B

更新日期：2014-11-25 00:00:00

abstract：BACKGROUND:New World vultures (Cathartiformes: Cathartidae) are obligate scavengers comprised of seven species in five genera throughout the Americas. Of these, turkey vultures (Cathartes aura) and black vultures (Coragyps atratus) are the most widespread and, although ecologically similar, have evolved differences in ...

journal_title：Malaria journal

authors： Yabsley MJ,Vanstreels RET,Martinsen ES,Wickson AG,Holland AE,Hernandez SM,Thompson AT,Perkins SL,West CJ,Bryan AL,Cleveland CA,Jolly E,Brown JD,McRuer D,Behmke S,Beasley JC

更新日期：2018-01-08 00:00:00

abstract：BACKGROUND:The objective of this project was to achieve high, sustainable levels of net coverage in a village in rural Tanzania by combining free distribution of long-lasting insecticide-impregnated nets (LLINs) with community-tailored education. In Tanzania, malaria is the leading cause of morbidity and mortality. Alt...

journal_title：Malaria journal

authors： Widmar M,Nagel CJ,Ho DY,Benziger PW,Hennig N

更新日期：2009-12-31 00:00:00

abstract：BACKGROUND:When discussing the relationship between meteorological factors and malaria, previous studies mainly focus on the interaction between different climatic factors, while the possible interaction within one particular climatic predictor at different lag periods has been largely neglected. In this study, this is...

journal_title：Malaria journal

authors： Wu Y,Qiao Z,Wang N,Yu H,Feng Z,Li X,Zhao X

更新日期：2017-01-31 00:00:00

abstract：BACKGROUND:Malaria transmission in arid and semi-arid regions of Kenya such as Baringo County, is seasonal and often influenced by climatic factors. Unravelling the relationship between climate variables and malaria transmission dynamics is therefore instrumental in developing effective malaria control strategies. The ...

journal_title：Malaria journal

authors： Kipruto EK,Ochieng AO,Anyona DN,Mbalanya M,Mutua EN,Onguru D,Nyamongo IK,Estambale BBA

更新日期：2017-05-25 00:00:00

abstract：BACKGROUND:Internally displaced persons (IDP) represent vulnerable populations whose public health conditions merit special attention. In the China-Myanmar border area, human movement and resettlements of IDP can influence malaria transmission. Comparison of disease incidence and vector densities between IDP camps and ...

journal_title：Malaria journal

authors： Zhou G,Lo E,Zhong D,Wang X,Wang Y,Malla S,Lee MC,Yang Z,Cui L,Yan G

更新日期：2016-09-15 00:00:00

abstract：:The goal to eliminate malaria from the Asia-Pacific by 2030 will require the safe and widespread delivery of effective radical cure of malaria. In October 2017, the Asia Pacific Malaria Elimination Network Vivax Working Group met to discuss the impediments to primaquine (PQ) radical cure, how these can be overcome and...

journal_title：Malaria journal

authors： Thriemer K,Bobogare A,Ley B,Gudo CS,Alam MS,Anstey NM,Ashley E,Baird JK,Gryseels C,Jambert E,Lacerda M,Laihad F,Marfurt J,Pasaribu AP,Poespoprodjo JR,Sutanto I,Taylor WR,van den Boogaard C,Battle KE,Dysoley L,Ghim

更新日期：2018-06-20 00:00:00

abstract：BACKGROUND:The erythrocytic stage of Plasmodium falciparum parasites in humans is clinically important, as the parasites at this growth stage causes malarial symptoms. Most of the currently available anti-malarial drugs target this stage, but the emergence and spread of parasites resistant to anti-malarial drugs are a ...

journal_title：Malaria journal

authors： Tougan T,Toya Y,Uchihashi K,Horii T

更新日期：2019-01-14 00:00:00

abstract：BACKGROUND:Early diagnosis and prompt treatment including appropriate home-based treatment of malaria is a major strategy for malaria control. A major determinant of clinical outcome in case management is compliance and adherence to effective antimalarial regimen. Home-based malaria treatment with inappropriate medicin...

journal_title：Malaria journal

authors： Orimadegun AE,Amodu OK,Olumese PE,Omotade OO

更新日期：2008-07-29 00:00:00

abstract：:BACKGROUND: This study investigated causes of malaria and how cases were managed at household level, in order to improve the ability to identify malaria and ensure correct use of chloroquine. It was conducted in Nakonde District, Northern Province of Zambia, between 2000 and 2001. Nakonde district is in a hyperendemic...

journal_title：Malaria journal

authors： Kaona FA,Tuba M

更新日期：2003-11-19 00:00:00

abstract：BACKGROUND:Post-malaria neurological syndrome (PMNS) is a debated entity, defined by neurological complications following a post-malaria symptom-free period and a negative blood smear. Four cases of PMNS are hereby reported and a review the literature performed to clarify the nosological framework of this syndrome. ME...

journal_title：Malaria journal

authors： Tamzali Y,Demeret S,Haddad E,Guillot H,Caumes E,Jauréguiberry S

更新日期：2018-10-26 00:00:00

abstract：BACKGROUND:This study was conducted to evaluate whether a selection of South African ethnomedicinal plants included in this study displayed insecticidal properties when screened against adult stages of the mosquito. METHODS:381 crude extracts of 80 plant taxa in 42 families were sprayed onto ceramic tiles and screened...

journal_title：Malaria journal

authors： Maharaj R,Maharaj V,Crouch NR,Bhagwandin N,Folb PI,Pillay P,Gayaram R

更新日期：2011-08-11 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the profile of antibodies against several antigens of Plasmodium vivax and Plasmodium falciparum in Mandalay, Myanmar. METHODS:Malaria parasites were identified by microscopic examination. To test the antibodies against P. vivax and P. falciparum in sera, an indirect...

journal_title：Malaria journal

authors： Kim TS,Kim HH,Kim JY,Kong Y,Na BK,Lin K,Moon SU,Kim YJ,Kwon MH,Sohn Y,Kim H,Lee HW

更新日期：2011-08-06 00:00:00

abstract：BACKGROUND:Appropriate case management of suspected malaria in Cambodia is critical given anti-malarial drug resistance in the region. Improving diagnosis and the use of recommended malarial treatments is a challenge in Cambodia where self-treatment and usage of drug cocktails is widespread, a notable difference from m...

journal_title：Malaria journal

authors： O'Connell KA,Samandari G,Phok S,Phou M,Dysoley L,Yeung S,Allen H,Littrell M

更新日期：2012-10-05 00:00:00

abstract：BACKGROUND:In view of the close relationship of Portugal with African countries, particularly former Portuguese colonies, the diagnosis of malaria is not a rare thing. When a traveller returns ill from endemic areas, malaria should be the number one suspect. World Health Organization treatment guidelines recommend that...

journal_title：Malaria journal

authors： Santos LC,Abreu CF,Xerinda SM,Tavares M,Lucas R,Sarmento AC

更新日期：2012-03-29 00:00:00

abstract：BACKGROUND:T1BT* is a peptide construct containing the T1 and B epitopes located in the 5' minor repeat and the 3' major repeat of the central repeat region of the Plasmodium falciparum circumsporozoite protein (CSP), respectively, and the universal T* epitope located in the C-terminus of the same protein. This peptide...

journal_title：Malaria journal

authors： Topchiy E,Armstrong GS,Boswell KI,Buchner GS,Kubelka J,Lehmann TE

更新日期：2013-03-18 00:00:00

abstract：BACKGROUND:The intermittent screening and treatment (IST) of school children for malaria is one possible intervention strategy that could help reduce the burden of malaria among school children. Future implementation of IST will not only depend on its efficacy and cost-effectiveness but also on its acceptability to par...

journal_title：Malaria journal

authors： Okello G,Ndegwa SN,Halliday KE,Hanson K,Brooker SJ,Jones C

更新日期：2012-06-08 00:00:00

abstract：BACKGROUND:Understanding spatio-temporal variation in malaria incidence provides a basis for effective disease control planning and monitoring. METHODS:Monthly surveillance data between 1991 and 2006 for Plasmodium vivax and Plasmodium falciparum malaria across 128 counties were assembled for Yunnan, a province of Chi...

journal_title：Malaria journal

authors： Clements AC,Barnett AG,Cheng ZW,Snow RW,Zhou HN

更新日期：2009-07-31 00:00:00

abstract：:The substantial impact of cross-border collaborative control efforts on the burden of malaria in southern Africa has previously been demonstrated through the successes of the Lubombo Spatial Development Initiative. Increases in malaria cases recorded in the three partner countries (Mozambique, South Africa, Swaziland)...

journal_title：Malaria journal

authors： Moonasar D,Maharaj R,Kunene S,Candrinho B,Saute F,Ntshalintshali N,Morris N

更新日期：2016-08-18 00:00:00

abstract：BACKGROUND:Intensified efforts are urgently needed to contain and eliminate artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion. Médecins Sans Frontières plans to support the Ministry of Health in eliminating P. falciparum in an area with artemisinin resistance in the north-east of Cambodia. As ...

journal_title：Malaria journal

authors： Bosman P,Stassijns J,Nackers F,Canier L,Kim N,Khim S,Alipon SC,Chuor Char M,Chea N,Dysoley L,Van den Bergh R,Etienne W,De Smet M,Ménard D,Kindermans JM

更新日期：2014-10-06 00:00:00

abstract：:BACKGROUND: Removal of exhaled air from total body emanations or artificially standardising carbon dioxide (CO2) outputs has previously been shown to eliminate differential attractiveness of humans to certain blackfly (Simuliidae) and mosquito (Culicidae) species. Whether or not breath contributes to between-person di...

journal_title：Malaria journal

authors： Mukabana WR,Takken W,Killeen GF,Knols BG

更新日期：2004-01-29 00:00:00

abstract：BACKGROUND:The emergence of parasite drug resistance, especially Plasmodium falciparum, persists as a major obstacle for malaria control and elimination. To develop effective public health containment strategies, a clear understanding of factors that govern the emergence and spread of resistant parasites in the field i...

journal_title：Malaria journal

authors： Mharakurwa S,Sialumano M,Liu K,Scott A,Thuma P

更新日期：2013-12-19 00:00:00