Abstract:
:Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of Plasmodium falciparum. Compared to standard protocols plate-based transfection requires 20-fold less DNA, transient transfection efficiency achieved is approximately seven-fold higher, whilst stable transfection success rate is above 90%. Furthermore the utility of this set of protocols to generate a knockout of the PfRH3 pseudogene, screened by whole-cell PCR, is demonstrated. The methods and tools presented here will facilitate genome-scale genetic manipulation of P. falciparum.
journal_name
Malar Jjournal_title
Malaria journalauthors
Caro F,Miller MG,DeRisi JLdoi
10.1186/1475-2875-11-22subject
Has Abstractpub_date
2012-01-18 00:00:00pages
22issn
1475-2875pii
1475-2875-11-22journal_volume
11pub_type
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