Abstract:
BACKGROUND:Plasmodium knowlesi is the fifth Plasmodium species that can infect humans. The Plasmodium merozoite surface protein-1(42) (MSP-1(42)) is a potential candidate for malaria vaccine. However, limited studies have focused on P. knowlesi MSP-1(42). METHODS:A ~42 kDa recombinant P. knowlesi MSP-1(42) (pkMSP-1(42)) was expressed using an Escherichia coli system. The purified pkMSP-1(42) was evaluated with malaria and non-malaria human patient sera (n = 189) using Western blots and ELISA. The immunogenicity of pkMSP-1(42) was evaluated in mouse model. RESULTS:The purified pkMSP-1(42) had a sensitivity of 91.0% for detection of human malaria in both assays. Specificity was 97.5 and 92.6% in Western blots and ELISA, respectively. Levels of cytokine interferon-gamma, interleukin-2, interleukin-4, and interleukin-10 significantly increased in pkMSP-1(42)-immunized mice as compared to the negative control mice. pkMSP-1(42)-raised antibody had high endpoint titres, and the IgG isotype distribution was IgG1 > IgG2b > IgG3 > IgG2a. CONCLUSIONS:pkMSP-1(42) was highly immunogenic and able to detect human malaria. Hence, pkMSP-1(42) would be a useful candidate for malaria vaccine development and seroprevalence studies.
journal_name
Malar Jjournal_title
Malaria journalauthors
Cheong FW,Fong MY,Lau YL,Mahmud Rdoi
10.1186/1475-2875-12-454subject
Has Abstractpub_date
2013-12-19 00:00:00pages
454issn
1475-2875pii
1475-2875-12-454journal_volume
12pub_type
杂志文章相关文献
MALARIA JOURNAL文献大全abstract:BACKGROUND:Malaria in highland areas of Kenya affects children and adults. Local clinicians include symptoms other than fever when screening for malaria because they believe that fever alone does not capture all cases of malaria. METHODS:Individuals who presented to dispensaries in a highland Kenya site of low, unstab...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-163
更新日期:2014-04-30 00:00:00
abstract:BACKGROUND:Passerine birds are frequently infected with diverse haemosporidian parasites. While infections are traditionally considered benign in wild birds, recent studies demonstrated mortalities of passerine species due to exo-erythrocytic development of the parasites, which can damage organs in affected hosts. Howe...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-3147-6
更新日期:2020-02-12 00:00:00
abstract:BACKGROUND:Insecticide resistance in Anopheles coluzzii mosquitoes has become widespread throughout West Africa including in Burkina Faso. The insecticide resistance allele (kdr or L1014F) is a prime indicator that is highly correlated with phenotypic resistance in West Africa. Studies from Benin, Ghana and Mali have s...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2759-1
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Surprisingly little is known about the geographical overlap between malaria and other tropical diseases, including helminth infections. This is despite the potential public health importance of co-infection and synergistic opportunities for control. METHODS:Statistical models are presented that predict the ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-5-99
更新日期:2006-11-03 00:00:00
abstract:BACKGROUND:Control of the major African malaria vector species continues to rely extensively on the application of residual insecticides through indoor house spraying or bed net impregnation. Insecticide resistance is undermining the sustainability of these control strategies. Alternatives to the currently available co...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-71
更新日期:2010-03-08 00:00:00
abstract:BACKGROUND:The study of the biology, transmission and pathogenesis of Plasmodium vivax is hindered due to the lack of a robustly propagating, continuous culture of this parasite. The current culture system for P. vivax parasites still suffered from consistency and difficulties in long-term maintenance of parasites in c...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0815-z
更新日期:2015-08-05 00:00:00
abstract:BACKGROUND:The use of insecticide-treated nets (ITN) is an important tool in the Roll Back Malaria (RBM) strategy. For ITNs to be effective they need to be used correctly. Previous studies have shown that many factors, such as wealth, access to health care, education, ethnicity and gender, determine the ownership and u...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-175
更新日期:2009-07-29 00:00:00
abstract:BACKGROUND:Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. METHODS:A total 606 febrile patients in the Ch...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-73
更新日期:2013-02-22 00:00:00
abstract:BACKGROUND:In formerly endemic areas where malaria transmission has declined, levels of population immunity to Plasmodium falciparum provide information on continued malaria transmission and potentially susceptible populations. Traditional techniques for measuring serological responses to P. falciparum antigens use pla...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-162
更新日期:2011-06-10 00:00:00
abstract:BACKGROUND:Polymorphisms within the PfATP6 gene have been indicated as potential molecular markers for artemisinin efficacy. Since 2004, the use of artemisinin combination therapy (ACT) was introduced as first-line treatment of the uncomplicated malaria cases in Suriname. The aim of this research was to determine chang...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-322
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:Improving the way artemether-lumefantrine (AL) is provided to patients attending clinics is critical to maximize the benefit of this new medicine. In 2007, a new initiative was launched in one part of Kenya to improve malaria case-management through enhanced in-service training and provision of job aids. ME...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-261
更新日期:2010-09-18 00:00:00
abstract:BACKGROUND:Following the recognition that morbidity and mortality due to malaria had dramatically increased in the last three decades, in 2002 the government of Zambia reviewed its efforts to prevent and treat malaria. Convincing evidence of the failing efficacy of chloroquine resulted in the initiation of a process th...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-25
更新日期:2008-01-29 00:00:00
abstract:BACKGROUND:Glucose 6-phosphate dehydrogenase (G6PD) is an enzyme involved in prevention of cellular oxidative damage, particularly protecting erythrocytes from haemolysis. An estimated 400 million people present variable degrees of inherited G6PD deficiency (G6PDd) which puts them at risk for developing haemolysis trig...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1343-1
更新日期:2016-05-26 00:00:00
abstract:BACKGROUND:The Cascades region, Burkina Faso, has a high malaria burden despite reported high insecticide-treated mosquito net (ITN) use. Human and vector activities outside the hours when indoor interventions offer direct protection from infectious bites potentially increase exposure risk to bites from malaria-transmi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03538-5
更新日期:2021-01-18 00:00:00
abstract::This article reports the changing pattern of US President's Malaria Initiative-funded IRS in sub-Saharan Africa between 2008 and 2015. IRS coverage in sub-Saharan Africa increased from <2 % of the at-risk population in 2005, to 11 % or 78 million people in 2010, mainly as a result of increased funding from PMI. The sc...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1201-1
更新日期:2016-03-08 00:00:00
abstract:BACKGROUND:Knowledge of the ecological differences between the molecular forms of Anopheles gambiae and their sibling species, An. arabiensis might lead to understanding their unique contribution to disease transmission and to better vector control as well as to understanding the evolutionary forces that have separated...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-5-19
更新日期:2006-03-22 00:00:00
abstract:BACKGROUND:Acute kidney injury (AKI) is a frequently encountered complication of imported Plasmodium falciparum infection. Markers of structural kidney damage have been found to detect AKI earlier than serum creatinine-based prediction models but have not yet been evaluated in imported malaria. This pilot study aims to...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1516-y
更新日期:2016-09-09 00:00:00
abstract:BACKGROUND:Malaria can be transmitted by blood transfusion through donations collected from asymptomatic or parasitic donors. The parasites are released into the bloodstream during its life cycle and will therefore be present in donated blood by infected individuals. All cases of transfusion-transmitted malaria (TTM) n...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2486-z
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:The development of insecticide resistance and the increased outdoor-biting behaviour of malaria vectors reduce the efficiency of indoor vector control methods. Attractive toxic sugar baits (ATSBs), a method targeting the sugar-feeding behaviours of vectors both indoors and outdoors, is a promising supplement...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-1012-9
更新日期:2015-12-08 00:00:00
abstract::Following publication of the original article [1], one of the authors has highlighted an xml-related discrepancy concerning the author group titled 'Additional Tracking Resistance to Artemisinin Collaboration authors (TRAC Group Authorship)', listed under the Acknowledgements section. ...
journal_title:Malaria journal
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12936-018-2464-5
更新日期:2018-09-10 00:00:00
abstract:BACKGROUND:There is evidence that suggests that undernutrition has a detrimental effect on malarial immunity in children. The aim of the study was to discover whether nutrient supplementation improved development of malarial antibody immunity in children up to 18 months of age. METHODS:The study was conducted with a s...
journal_title:Malaria journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12936-018-2224-6
更新日期:2018-02-07 00:00:00
abstract:BACKGROUND:In Plasmodium, the high level of genetic diversity and the interactions established by co-infecting parasite populations within the same host may be a source of selection on pathogen virulence and drug resistance. As different patterns have already been described in humans and mosquitoes, parasite diversity ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-114
更新日期:2013-03-27 00:00:00
abstract:BACKGROUND:In recent years, malaria (Plasmodium vivax and Plasmodium falciparum) has been successfully controlled in the Ecuador-Peru coastal border region. The aim of this study was to document this control effort and to identify the best practices and lessons learned that are applicable to malaria control and to othe...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1630-x
更新日期:2016-11-28 00:00:00
abstract:BACKGROUND:New World vultures (Cathartiformes: Cathartidae) are obligate scavengers comprised of seven species in five genera throughout the Americas. Of these, turkey vultures (Cathartes aura) and black vultures (Coragyps atratus) are the most widespread and, although ecologically similar, have evolved differences in ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-2165-5
更新日期:2018-01-08 00:00:00
abstract:BACKGROUND:Acute kidney injury (AKI) is a common complication of malaria. In low resource settings, a lack of diagnostic tools and delayed treatment of malaria associated AKI lead to significant morbidity and mortality. The aim of this study was to assess the diagnostic performance of salivary urea nitrogen (SUN) dipst...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2627-4
更新日期:2018-12-18 00:00:00
abstract:BACKGROUND:Complex malaria infections are defined as those containing more than one genetically distinct lineage of Plasmodium parasite. Complexity of infection (COI) is a useful parameter to estimate from patient blood samples because it is associated with clinical outcome, epidemiology and disease transmission rate. ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-14-4
更新日期:2015-01-19 00:00:00
abstract:BACKGROUND:Relatively few programmes have attempted to actively engage the private sector in national malaria control efforts. This paper evaluates the health impact of a large-scale distribution of insecticide-treated nets (ITNs) conducted in partnership with a Zambian agribusiness, and its cost-effectiveness from the...
journal_title:Malaria journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/1475-2875-12-102
更新日期:2013-03-18 00:00:00
abstract:BACKGROUND:There is limited data available regarding safety profile of artemisinins in early pregnancy. They are, therefore, not recommended by WHO as a first-line treatment for malaria in first trimester due to associated embryo-foetal toxicity in animal studies. The study assessed birth outcome among pregnant women i...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-197
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:Anti-malarial herbal preparations (HPs) continue to enjoy high patronage in Ghana despite reports that the artemisinin-based combination therapy (ACT), the recommended first choice for treatment of uncomplicated malaria in the country, remains efficacious. A major issue with the use of these preparations is ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1757-4
更新日期:2017-03-04 00:00:00
abstract::Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of Plasmodium falciparum. Compared to standard protocols plate-based t...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-22
更新日期:2012-01-18 00:00:00