Abstract:
BACKGROUND:The merozoite surface protein 1 (msp1) is one of the most studied vaccine candidate genes in mammalian Plasmodium spp. to have been used for investigations of epidemiology, population structures, and immunity to infections. However methodological difficulties have impeded the use of nuclear markers such as msp1 in Plasmodium parasites causing avian malaria. Data from an infection transcriptome of the host generalist avian malaria parasite Plasmodium relictum was used to identify and characterize the msp1 gene from two different isolates (mtDNA lineages SGS1 and GRW4). The aim was to investigate whether the msp1 gene in avian malaria species shares the properties of the msp1 gene in Plasmodium falciparum in terms of block variability, conserved anchor points and repeat motifs, and further to investigate the degree to which the gene might be informative in avian malaria parasites for population and epidemiological studies. METHODS:Reads from 454 sequencing of birds infected with avian malaria was used to develop Sanger sequencing protocols for the msp1 gene of P. relictum. Genetic variability between variable and conserved blocks of the gene was compared within and between avian malaria parasite species, including P. falciparum. Genetic variability of the msp1 gene in P. relictum was compared with six other nuclear genes and the mtDNA gene cytochrome b. RESULTS:The msp1 gene of P. relictum shares the same general pattern of variable and conserved blocks as found in P. falciparum, although the variable blocks exhibited less variability than P. falciparum. The variation across the gene blocks in P. falciparum spanned from being as conserved as within species variation in P. relictum to being as variable as between the two avian malaria species (P. relictum and Plasmodium gallinaceum) in the variable blocks. In P. relictum the highly conserved p19 region of the peptide was identified, which included two epidermal growth factor-like domains and a fully conserved GPI anchor point. CONCLUSION:This study provides protocols for evaluation of the msp1 gene in the avian malaria generalist parasite P. relictum. The msp1 gene in avian Plasmodium shares the genetic properties seen in P. falciparum, indicating evolutionary conserved functions for the gene. The data on the variable blocks of the gene show that the msp1 gene in P. relictum might serve as a good candidate gene for future population and epidemiological studies of the parasite.
journal_name
Malar Jjournal_title
Malaria journalauthors
Hellgren O,Kutzer M,Bensch S,Valkiūnas G,Palinauskas Vdoi
10.1186/1475-2875-12-381subject
Has Abstractpub_date
2013-10-30 00:00:00pages
381issn
1475-2875pii
1475-2875-12-381journal_volume
12pub_type
杂志文章相关文献
MALARIA JOURNAL文献大全abstract:BACKGROUND:Deltamethrin-impregnated, long-lasting insecticidal nets (LLINs) were distributed in the study area from November 2014 to January 2015 to evaluate their impact on malaria transmission in the presence of insecticide-resistant vectors. Studies were carried out in 16 selected clusters in Keshkal sub-district, C...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2557-1
更新日期:2018-11-08 00:00:00
abstract:BACKGROUND:Malaria control has been dramatically scaled up the past decade, mainly thanks to increasing international donor financing since 2003. This study assessed progress up to 2010 towards global malaria impact targets, in relation to Global Fund, other donor and domestic malaria programme financing over 2003 to 2...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-18
更新日期:2013-01-14 00:00:00
abstract:BACKGROUND:Following the recognition that morbidity and mortality due to malaria had dramatically increased in the last three decades, in 2002 the government of Zambia reviewed its efforts to prevent and treat malaria. Convincing evidence of the failing efficacy of chloroquine resulted in the initiation of a process th...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-25
更新日期:2008-01-29 00:00:00
abstract:BACKGROUND:Anopheles arabiensis is a dominant vector of malaria in sub-Saharan Africa, which feeds indoors and outdoors on human and other vertebrate hosts, making it a difficult species to control with existing control methods. Novel methods that reduce human-vector interactions are, therefore, required to improve the...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1386-3
更新日期:2016-07-21 00:00:00
abstract:BACKGROUND:Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1) antigens play a critical role in host immune evasion. Serologic responses to these antigens have been associated with protection from clinical malaria, suggesting that antibodies to PfEMP1 antigens may contribute to natural immunity. The first N-t...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2905-9
更新日期:2019-08-13 00:00:00
abstract:BACKGROUND:Understanding spatio-temporal variation in malaria incidence provides a basis for effective disease control planning and monitoring. METHODS:Monthly surveillance data between 1991 and 2006 for Plasmodium vivax and Plasmodium falciparum malaria across 128 counties were assembled for Yunnan, a province of Chi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-180
更新日期:2009-07-31 00:00:00
abstract:BACKGROUND:Malaria is a global health emergency, and yet our understanding of the energy metabolism of the principle causative agent of this devastating disease, Plasmodium falciparum, remains rather basic. Glucose was shown to be an essential nutritional requirement nearly 100 years ago and since this original observa...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-38
更新日期:2009-03-06 00:00:00
abstract:BACKGROUND:Mass distribution campaigns of insecticide-treated nets for malaria prevention are usually accompanied by intensive behaviour change communication (BCC) to encourage hanging and use of nets. However, data on the effectiveness of these communication efforts are scarce. In preparation for the next round of mas...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1463-7
更新日期:2016-08-19 00:00:00
abstract:BACKGROUND:Laboratory studies have demonstrated that a variety of immune signaling pathways regulate malaria parasite infection in Anopheles gambiae, the primary vector species in Africa. METHODS:To begin to understand the importance of these associations under natural conditions, an association mapping approach was a...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-160
更新日期:2010-06-11 00:00:00
abstract:BACKGROUND:Glucose 6-phosphate dehydrogenase (G6PD) is an enzyme involved in prevention of cellular oxidative damage, particularly protecting erythrocytes from haemolysis. An estimated 400 million people present variable degrees of inherited G6PD deficiency (G6PDd) which puts them at risk for developing haemolysis trig...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1343-1
更新日期:2016-05-26 00:00:00
abstract:BACKGROUND:Relatively few programmes have attempted to actively engage the private sector in national malaria control efforts. This paper evaluates the health impact of a large-scale distribution of insecticide-treated nets (ITNs) conducted in partnership with a Zambian agribusiness, and its cost-effectiveness from the...
journal_title:Malaria journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/1475-2875-12-102
更新日期:2013-03-18 00:00:00
abstract:BACKGROUND:The massive deployment of life saving malaria interventions has not only resulted in a decline in disease burden but a change in the risk of infection and disease. The study reassesses the importance of known risk factors and reviews demographic and socio-economic determinants of malaria risk in the populati...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-306
更新日期:2014-08-08 00:00:00
abstract:BACKGROUND:Many recent studies have examined the impact of urbanization on Plasmodium falciparum malaria endemicity and found a general trend of reduced transmission in urban areas. However, none has examined the effect of urbanization on Plasmodium vivax malaria, which is the most widely distributed malaria species an...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-403
更新日期:2012-12-05 00:00:00
abstract:BACKGROUND:Several anti-malarial drugs are associated with adverse cardiovascular effects. These effects may be exacerbated when different anti-malarials are used in combination. There has been no report yet on the potential cardiac effects of the combination artesunate-amodiaquine. METHODS:Electrocardiographic (ECG) ...
journal_title:Malaria journal
pub_type: 临床试验,杂志文章
doi:10.1186/1475-2875-11-420
更新日期:2012-12-17 00:00:00
abstract:BACKGROUND:Iron deficiency is the most widespread nutrient deficiency and an important cause of developmental impairment in children. However, some studies have indicated that iron deficiency can also protect against malaria, which is a leading cause of childhood morbidity and mortality in large parts of the world. Thi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2186-8
更新日期:2018-01-16 00:00:00
abstract:BACKGROUND:A high-resolution surveillance-response system has been developed within a geographic information system (GIS) to support malaria elimination in the Pacific. This paper examines the application of a GIS-based spatial decision support system (SDSS) to automatically locate and map the distribution of confirmed...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-108
更新日期:2013-03-21 00:00:00
abstract:BACKGROUND:In a previous study, severe and cerebral malaria have been connected with acute cochlear malfunction in children, demonstrated by a decrease of transitory evoked otoacoustic emissions (TEOAEs) reproducibility. This study aims to determine whether cochlear malfunction persists for 4 years after recovery from ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2840-9
更新日期:2019-06-24 00:00:00
abstract:BACKGROUND:This study aimed to investigate baseline data on malaria before the evaluation of new vector control strategies in an area of pyrethroid-resistance of vectors. The burden of malaria was estimated in terms of infection (prevalence and parasite density) and of clinical episodes. METHODS:Between December 2007 ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-380
更新日期:2010-12-31 00:00:00
abstract::The potential use of ivermectin as an additional vector control tool is receiving increased attention from the malaria elimination community, driven by the increased importance of outdoor/residual malaria transmission and the threat of insecticide resistance where vector tools have been scaled-up. This report summariz...
journal_title:Malaria journal
pub_type:
doi:10.1186/s12936-015-0691-6
更新日期:2015-06-11 00:00:00
abstract:BACKGROUND:Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistanc...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03246-0
更新日期:2020-05-08 00:00:00
abstract:BACKGROUND:Passerine birds are frequently infected with diverse haemosporidian parasites. While infections are traditionally considered benign in wild birds, recent studies demonstrated mortalities of passerine species due to exo-erythrocytic development of the parasites, which can damage organs in affected hosts. Howe...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-3147-6
更新日期:2020-02-12 00:00:00
abstract:BACKGROUND:Molecular genotyping is performed in anti-malarial trials to determine whether recurrent parasitaemia after therapy represents a recrudescence (treatment failure) or new infection. The use of capillary instead of agarose gel electrophoresis for genotyping offers technical advantages, but it is unclear whethe...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-19
更新日期:2010-01-15 00:00:00
abstract::Malaria currently causes more harm to human beings than any other parasitic disease, and disproportionally affects low-income populations. The ethical issues raised by efforts to control or eliminate malaria have received little explicit analysis, in comparison with other major diseases of poverty. While some ethical ...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/s12936-015-1042-3
更新日期:2015-12-22 00:00:00
abstract:BACKGROUND:Antananarivo, the capital of Madagascar, is located at an altitude of over 1,200 m. The environment at this altitude is not particularly favourable to malaria transmission, but malaria nonetheless remains a major public health problem. The aim of this study was to evaluate exposure to malaria in the urban po...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-5-106
更新日期:2006-11-10 00:00:00
abstract:BACKGROUND:Although chemoprophylaxis remains an important strategy for preventing malaria in travellers, its effectiveness may be compromised by lack of adherence. Inappropriate use of chemoprophylaxis is likely to increase the risk of acquiring malaria, but may probably also worsen the severity of imported cases. The ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-265
更新日期:2013-07-31 00:00:00
abstract::Malaria vaccines are considered amongst the most important modalities for potential elimination of malaria disease and transmission. Research and development in this field has been an area of intense effort by many groups over the last few decades. Despite this, there is currently no licensed malaria vaccine. Research...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-12-86
更新日期:2013-03-04 00:00:00
abstract:BACKGROUND:The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-3142-y
更新日期:2020-02-03 00:00:00
abstract:BACKGROUND:Increases in the coverage of long-lasting insecticidal nets (LLINs) have significantly reduced the abundance of Anopheles gambiae sensu stricto in several African settings, leaving its more zoophagic sibling species Anopheles arabiensis as the primary vector. This study investigated the impact of livestock o...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-014-0536-8
更新日期:2015-01-21 00:00:00
abstract:BACKGROUND:Indonesia is home to a variety of malaria vectors whose specific bionomic traits remain largely uncharacterized. Species-specific behaviours, such as host feeding preferences, impact the dynamics of malaria transmission and the effectiveness of vector control interventions. METHODS:To examine species-specif...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1950-5
更新日期:2017-08-02 00:00:00
abstract:BACKGROUND:Malaria remains a major human health problem, with no licensed vaccine currently available. Malaria infections initiate when infectious Plasmodium sporozoites are transmitted by Anopheline mosquitoes during their blood meal. Investigations of the malaria sporozoite are, therefore, of clear medical importance...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-421
更新日期:2012-12-17 00:00:00