Abstract:
BACKGROUND:The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated with host-vector transmission. This highlights the importance of studying the parasite's sexual forms (gametocytes) which are involved in this phase of the parasite's life-cycle. Some African and Asian strains of Plasmodium falciparum have been fully characterized regarding sexual forms' production; however, few Latin-American strains have been so characterized. This study was aimed at characterizing the Colombian FCB2 strain as a gametocyte producer able to infect mosquitoes. METHODS:Gametocyte production was induced in in vitro cultured P. falciparum FCB2 and 3D7 strains. Pfap2g and Pfs25 gene expression was detected in FCB2 strain gametocyte culture by RT-PCR. Comparative analysis of gametocytes obtained from both strains was made (counts and morphological changes). In vitro zygote formation from FCB2 gametocytes was induced by incubating a gametocyte culture sample at 27 °C for 20 min. A controlled Anopheles albimanus infection was made using an artificial feed system with cultured FCB2 gametocytes (14-15 days old). Mosquito midgut dissection was then carried out for analyzing oocysts. RESULTS:The FCB2 strain expressed Pfap2g, Pfs16, Pfg27/25 and Pfs25 sexual differentiation-related genes after in vitro sexual differentiation induction, producing gametocytes that conserved the expected morphological features. The amount of FCB2 gametocytes produced was similar to that from the 3D7 strain. FCB2 gametocytes were differentiated into zygotes and ookinetes after an in vitro low-temperature stimulus and infected An. albimanus mosquitoes, developing to oocyst stage. CONCLUSIONS:Even with the history of long-term FCB2 strain in vitro culture maintenance, it has retained its sexual differentiation ability. The gametocytes produced here preserved these parasite forms' usual characteristics and An. albimanus infection capability, thus enabling its use as a tool for studying sexual form biology, An. albimanus infection comparative analysis and anti-malarial drug and vaccine development.
journal_name
Malar Jjournal_title
Malaria journalauthors
Ararat-Sarria M,Prado CC,Camargo M,Ospina LT,Camargo PA,Curtidor H,Patarroyo MAdoi
10.1186/s12936-020-3142-ysubject
Has Abstractpub_date
2020-02-03 00:00:00pages
57issue
1issn
1475-2875pii
10.1186/s12936-020-3142-yjournal_volume
19pub_type
杂志文章相关文献
MALARIA JOURNAL文献大全abstract:BACKGROUND:Drug resistance in Plasmodium falciparum is common in many endemic and other settings but there is no clear recommendation on when to change therapy when there is delay in parasite clearance after initiation of therapy in African children. METHODS:The factors contributing to delay in parasite clearance, def...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-53
更新日期:2010-02-15 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12936-016-1486-0.]. ...
journal_title:Malaria journal
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12936-016-1544-7
更新日期:2016-09-27 00:00:00
abstract:BACKGROUND:There is limited data available regarding safety profile of artemisinins in early pregnancy. They are, therefore, not recommended by WHO as a first-line treatment for malaria in first trimester due to associated embryo-foetal toxicity in animal studies. The study assessed birth outcome among pregnant women i...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-197
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:Malaria is endemic in Garo hills of Meghalaya, and death cases are reported annually. Plasmodium falciparum is the major parasite, and is solely responsible for each malaria-attributable death case. Garo hills are categorized high-risk for drug-resistant malaria; however, there exists no data on malaria tran...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-263
更新日期:2010-09-22 00:00:00
abstract:BACKGROUND:Described here is the first population genetic study of Plasmodium malariae, the causative agent of quartan malaria. Although not as deadly as Plasmodium falciparum, P. malariae is more common than previously thought, and is frequently in sympatry and co-infection with P. falciparum, making its study increas...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-38
更新日期:2011-02-11 00:00:00
abstract::This opinion article deals with the diagnostic clinical challenges faced by clinicians or health care workers in malaria-endemic areas when a severely sick child presents to the clinic with fever, coma or respiratory distress. Indeed, the coexistence of malaria with other severe infections like meningitis, invasive ba...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2533-9
更新日期:2018-10-22 00:00:00
abstract::India has committed to eliminate malaria by 2030. The national framework for malaria elimination released by the Government of India plans to achieve this goal through strategic planning in a phased manner. Since vector control is a major component of disease management and vector elimination, it requires a thorough u...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/s12936-019-3011-8
更新日期:2019-12-03 00:00:00
abstract:BACKGROUND:Malaria remains a worldwide public health concern and, in Colombia, despite the efforts to stop malaria transmission, the incidence of cases has increased over the last few years. In this context, it is necessary to evaluate vector diversity, infection rates, and spatial distribution, to better understand di...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-2076-5
更新日期:2017-10-27 00:00:00
abstract:BACKGROUND:Polyclonal B-cell activation is well known to occur in Plasmodium infections, but its role in pathogenesis or protection remains unclear. However, protective properties of natural antibodies have previously been demonstrated in other contexts. METHODS:Sera from asymptomatic and symptomatic Plasmodium-infect...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-4-5
更新日期:2005-01-20 00:00:00
abstract:BACKGROUND:The objective of this project was to achieve high, sustainable levels of net coverage in a village in rural Tanzania by combining free distribution of long-lasting insecticide-impregnated nets (LLINs) with community-tailored education. In Tanzania, malaria is the leading cause of morbidity and mortality. Alt...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-315
更新日期:2009-12-31 00:00:00
abstract:BACKGROUND:Successful reduction of malaria transmission to very low levels has made Isabel Province, Solomon Islands, a target for early elimination by 2014. High malaria transmission in neighbouring provinces and the potential for local asymptomatic infections to cause malaria resurgence highlights the need for sub-na...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-101
更新日期:2012-03-30 00:00:00
abstract:BACKGROUND:Extensive genetic diversity in the Plasmodium falciparum circumsporozoite protein (PfCSP) is a major contributing factor to the moderate efficacy of the RTS,S/AS01 vaccine. The transmission intensity and rates of recombination within and between populations influence the extent of its genetic diversity. Unde...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03510-3
更新日期:2020-11-27 00:00:00
abstract:BACKGROUND:Major investments have been made since 2001, with intensification of malaria control interventions after 2006. Interventions included free distribution of insecticide-treated nets (ITN) to pregnant women and children under 5 years old, the introduction of artemisinin combination therapy (ACT) for malaria tre...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2573-1
更新日期:2018-11-14 00:00:00
abstract:BACKGROUND:Models of Plasmodium falciparum malaria epidemiology that provide realistic quantitative predictions of likely epidemiological outcomes of existing vector control strategies have the potential to assist in planning for the control and elimination of malaria. This work investigates the applicability of mathem...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-357
更新日期:2012-10-29 00:00:00
abstract:BACKGROUND:Malaria remains highly endemic in Cameroon. The rapid emergence and spread of drug resistance was responsible for the change from monotherapies to artemisinin-based combinations. This systematic review and meta-analysis aimed to determine the prevalence and distribution of Plasmodium falciparum drug resistan...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03543-8
更新日期:2021-01-09 00:00:00
abstract:BACKGROUND:Malaria in pregnancy is associated with immunological abnormalities in the newborns, such as hampered T-helper 1 responses and increased T-regulatory responses, while the effect of maternal Plasmodium falciparum infection on foetal innate immunity is still controversial. MATERIALS AND METHODS:The immunophen...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-251
更新日期:2009-11-05 00:00:00
abstract:BACKGROUND:Development of resistance to different classes of insecticides is a potential threat to malaria control. With the increasing coverage of long-lasting insecticide-treated nets in Tanzania, the continued monitoring of resistance in vector populations is crucial. It may facilitate the development of novel strat...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-193
更新日期:2010-07-07 00:00:00
abstract:BACKGROUND:Mexico is in the malaria pre-elimination phase; therefore, continuous assessment and understanding of the social and behavioural risk factors related to exposure to malaria are necessary to achieve the overall goal. The aim of this research was to investigate socio-economic backgrounds, attitudes and practic...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-157
更新日期:2014-04-23 00:00:00
abstract:BACKGROUND:As a result of the reintroduction of malaria in the Republic of Korea (ROK) in 1993 and the threat to military and civilian populations, the Korea Military National Defense (MND) increased emphasis on vector control in 2012 at ROK Army (ROKA) installations located near the DMZ, while decreasing chemoprophyla...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1301-y
更新日期:2016-05-05 00:00:00
abstract:BACKGROUND:Heads of Government from Asia and the Pacific have committed to a malaria-free region by 2030. In 2015, the total number of confirmed cases reported to the World Health Organization by 22 Asia Pacific countries was 2,461,025. However, this was likely a gross underestimate due in part to incidence data not be...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1774-3
更新日期:2017-03-21 00:00:00
abstract:BACKGROUND:Complex malaria infections are defined as those containing more than one genetically distinct lineage of Plasmodium parasite. Complexity of infection (COI) is a useful parameter to estimate from patient blood samples because it is associated with clinical outcome, epidemiology and disease transmission rate. ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-14-4
更新日期:2015-01-19 00:00:00
abstract:BACKGROUND:The conserved centromere-associated proteins, CENH3 (or CENP-A) and CENP-C are indispensable for the functional centromere-kinetochore assembly, chromosome segregation, cell cycle progression, and viability. The presence and functions of centromere proteins in Plasmodium falciparum are not well studied. Iden...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-475
更新日期:2014-12-04 00:00:00
abstract:BACKGROUND:A recent randomized trial showed that artemisinin-naphthoquine (AN) was non-inferior to artemether-lumefantrine (AL) for falciparum malaria and superior for vivax malaria in young Papua New Guinean children. The aim of this study was to compare the cost-effectiveness of these two regimens. METHODS:An increm...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-2081-8
更新日期:2017-10-30 00:00:00
abstract:BACKGROUND:This study aimed to investigate baseline data on malaria before the evaluation of new vector control strategies in an area of pyrethroid-resistance of vectors. The burden of malaria was estimated in terms of infection (prevalence and parasite density) and of clinical episodes. METHODS:Between December 2007 ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-380
更新日期:2010-12-31 00:00:00
abstract:BACKGROUND:This analysis examined how the proportion of children less than 5-years-old who slept under a bed net the previous night changed during and after a national long-lasting insecticidal net (LLIN) distribution campaign in Sierra Leone in November-December 2010. METHODS:A citywide cross-sectional study in 2010-...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0990-y
更新日期:2015-11-18 00:00:00
abstract:BACKGROUND:Malaria rapid diagnostic tests (RDTs) are assumed to be simple-to-use and mobile technologies that have the capacity to standardize parasitological diagnosis for malaria across a variety of clinical settings. In order to evaluate these tests, it is important to consider how such assumptions play out in pract...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1083-2
更新日期:2016-02-05 00:00:00
abstract:BACKGROUND:Seasonal malaria chemoprevention (SMC) is a new strategy to reduce malaria burden in young children in Sahelian countries. It consists of the administration of full treatment courses of sulfadoxine-pyrimethamine plus amodiaquine to children at monthly intervals during the malaria season. However, it is not c...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1935-4
更新日期:2017-07-18 00:00:00
abstract:BACKGROUND:The objective of this study was to identify attitudes and misconceptions related to acceptance or refusal of indoor residual spraying (IRS) in Tanzania for both the general population and among certain groups (e.g., farmers, fishermen, community leaders, and women). METHODS:This study was a series of qualit...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-220
更新日期:2012-07-02 00:00:00
abstract:BACKGROUND:Malaria caused by Plasmodium ovale spp. has been neglected by and large from research and has received only little scientific attention during the past decades. Ovale malaria is considered to feature relapses by liver hypnozoites although scientific evidence for this paradigm is scarce. CASE PRESENTATION:He...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2226-4
更新日期:2018-02-09 00:00:00
abstract:BACKGROUND:Malaria is one of the most severe public health issues that result in massive morbidity and mortality in most countries of the sub-Saharan Africa (SSA). This study aimed to determine the scope of household, accessibility to malaria care and factors associated with household malaria in the Democratic Republic...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2679-0
更新日期:2019-02-26 00:00:00