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Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.

Abstract：

:Determination of CD34+ cells was performed in bone marrow and G-CSF mobilised peripheral blood samples. We adopted three different protocols of analysis: the Milan/Mulhouse protocol, the ISHAGE guidelines for CD34+ cell determination and our own protocol based upon the use of PAINT-A-GATEPRO software analysis program. An excellent correlation was demonstrated between the three methods (r2 0.98); however the analysis of variance showed a statistically significant difference between the results generated with the three methods (P=0.001). The differences between the three procedures are discussed with a special focus on the value of CD34+dim cells and the role of CD45 in the setting of a double staining. We have in fact identified a minor subset (CD34+CD38+CD45-) which would go unrecognised based upon its CD45 negativity.

journal_name

Int J Mol Med

journal_title

International journal of molecular medicine

authors

Venditti A,Buccisano F,Del Poeta G,Battaglia A,Tamburini A,Bruno A,Adorno G,Caravita T,Santinelli S,Del Moro B,Epiceno AM,Ballatore G,Amadori S

doi

10.3892/ijmm.1.1.67

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

67-70

issue

1

eissn

1107-3756

issn

1791-244X

journal_volume

1

pub_type

杂志文章

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