Abstract:
:Clinical observations and our high-density oligonucleotide microarray results demonstrated increased expression of proinflammatory chemokines after SARS-CoV infection. Here, we investigated the influence of SARS-CoV infection on CXCL8 (interleukin 8) and CXCL10 (interferon-gamma-inducible protein 10) in human intestinal epithelial (Caco2) cells. RT-PCR and ELISA showed time-dependent up-regulation of both chemokines after SARS-CoV infection. Electric mobility shift assay revealed increased DNA binding activity of the cellular transcription factors activator protein 1 (AP-1) and nuclear factor (B (NF-kappaB) in SARS-CoV infected cells. High hydrocortisone concentrations (> or =50 microg/ml) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10, but did not reduce chemokine expression to basal levels. Ribavirin that does not inhibit SARS-CoV replication in Vero cells inhibited SARS-CoV replication in Caco2 cells at therapeutical concentrations. Hydrocortisone neither influenced SARS-CoV titres alone nor in combination with ribavirin. Our results show that corticosteroids may be of limited benefit in the suppression of chemokine production by SARS-CoV-infected cells.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Cinatl J Jr,Michaelis M,Morgenstern B,Doerr HWsubject
Has Abstractpub_date
2005-02-01 00:00:00pages
323-7issue
2eissn
1107-3756issn
1791-244Xjournal_volume
15pub_type
杂志文章abstract::Hydrogen sulfide (H2S) is an endogenous gaseous biological mediator, which regulates, among others, the oxidative balance of cells under normal physiological conditions, as well as in various diseases. Several previous studies have reported that H2S attenuates inflammatory mediator production. In this study, we invest...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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abstract::Arsenic trioxide (ATO) can regulate many biological functions such as apoptosis and differentiation. We evaluated the effects of ATO on various cell types such as cervical cancer HeLa cells, pulmonary adenocarcinoma Calu-6 and A549 cells, calf pulmonary artery endothelial cells (CPAEC), human umbilical vein endothelia...
journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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abstract::Our earlier reports indicated that the changes of age-adjusted incidence rates (AAIRs) of any 2 tumors in time and space, as investigated by the sequential regression analysis, showed a good fitness to the equilibrium model under the control of the law of mass action. The purpose of this study is to investigate the pr...
journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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journal_title:International journal of molecular medicine
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abstract::The development of angiogenesis within a tumor brings on a sequence of extremely complex molecular events. We have developed a methodology which enables a wide set of biological parameters to be quantitatively determined in the field of anti-angiogenesis pharmacology. This methodology which includes a video cell track...
journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2011.612
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abstract::Immortalized cloned human chondrocytes isolated from a normal (Ch-4, 8, N) and an osteoarthritis patient (Ch-8-OA) were established by introduction of recombinant SV40-adenovirus vector containing SV40 early gene. These cells exhibited continuous proliferative capacity in monolayer culture and showed chondrocytic char...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.8.4.345
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journal_title:International journal of molecular medicine
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abstract::Choroidal neovascularization (CNV) can lead to progressive and severe visual loss. Vascular endothelial growth factor (VEGF) promotes the development of CNV. Caffeic acid phenethyl ester (CAPE), a biologically active component of the honeybee (Apis mellifera) propolis, has been demonstrated to have several interesting...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2015.2116
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journal_title:International journal of molecular medicine
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doi:
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2016.2835
更新日期:2017-02-01 00:00:00
abstract::In contrast to healthy intervertebral discs (IVDs), degenerate IVDs become vascularized. Here, we determined the role of an angiogenesis promoter, angiopoietin (Ang)-2, in the pathology of IVD degeneration (IDD). We evaluated degree of IDD using the Pfirrmann grading system. We used quantitative real-time polymerase c...
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
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