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A long distance-PCR derived FISH probe detects a deletion between p15 and p16 in CML and T-ALL patients.

Abstract：

:The tumor suppressor genes p15INK4B and p16INK4A, located in the chromosomal region 9p21, are frequently inactivated by homo- or hemizygous deletions, point mutation or promotor methylation in various types of cancer. No commercial probe is yet available that allows the detection of such deletions by FISH. Long distance (LD)-PCR was successfully used to generate a FISH probe, that covers a sequence stretch of 11.68 kb, located between the tumor suppressor genes p15 and p16. The LD-PCR amplicon was cloned and biotinylated by DOP-PCR (degenerated oligonucleotide primed-PCR) or nick translation. The FISH probe was hybridized on different samples of 16 patients with leukemia (3 T-ALL, 13 CML) and normal controls. Loss of at least one FISH-signal was found in 2/3 (67%) of the T-ALL- and 2/13 (15%) of the CML-cases. The new FISH probe presented here was proven to be advantageous for the detection of deletions in chromosomal region 9p21, especially between p15 and p16.

journal_name

Int J Mol Med

journal_title

International journal of molecular medicine

authors

Bleichert A,Fiedler W,Claussen U,Ernst G,Loncarevic IF,Heller A,Liehr T,Kunert C,von Eggeling F

doi

10.3892/ijmm.7.6.591

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

591-5

issue

6

eissn

1107-3756

issn

1791-244X

journal_volume

7

pub_type

杂志文章

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