Effects of trichostatin A, a histone deacetylase inhibitor, on the regulation of apoptosis in H-ras-transformed breast epithelial cells.


:This study examined the mechanism for the anti-cancer effects of histone deacetylase (HDAC) inhibitor trichostatin A (TsA) in H-ras-transformed human breast epithelial (MCF10A-ras) cells. The effects of TsA on anti-cancer effects of MCF10A-ras cells were determined by measuring the level of cell cycle regulator expression and apoptotic cell death using Western blotting and flow cytometry analysis, respectively. TsA induced morphological changes, apoptotic cell death and modulation of the cell cycle regulatory proteins in the MCF10A-ras cells. TsA increased the levels of acetylated histone H3 and H4 in MCF10A-ras cells. In addition, TsA markedly down-regulated the expression of cyclin D1 and CDK4, up-regulated the expression of p21WAF1 and p53 and induced cell cycle arrest at the G1 phase in MCF10A-ras cells. The levels of hyperphosphorylation of the Rb protein were lower in MCF10A-ras cells after the TsA treatment. Furthermore, the up-regulation of p53 promoted Bax expression, which led to the activation of pro-caspase-3 and eventually to apoptosis in MCF10A-ras cells. TsA significantly increased the levels of ERK1/2 phosphorylation in MCF10A-ras cells. Overall, the TsA-activated ERK pathway plays an important role in cell cycle arrest and apoptosis through the ERK-dependent induction of p21 in Ras-related human cancer cells.


Int J Mol Med


Park H,Lee YJ,Kim TH,Lee J,Yoon S,Choi WS,Myung CS,Kim HS


Has Abstract


2008-11-01 00:00:00












  • 8-(3-oxo-4,5,6-trihydroxy-3h-xanthen-9-yl)-1-naphthoic acid inhibits MAPK phosphorylation in endothelial cells induced by VEGF and bFGF.

    abstract::Angiogenesis is regulated by various factors. In particular, VEGF and basic FGF are of much importance. We found that 8-(3-oxo-4,5,6-trihydroxy-3h-xanthen-9-yl)-1-naphthoic acid inhibited the binding of VEGF to KDR/Flk-1 (VEGF receptor-2) or Flt-1 (VEGF receptor-1) and that it inhibited the MAPK phosphorylation in HUV...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Igarashi K,Isohara T,Kato T,Takigawa K,Shigeta K,Yamano T,Uno I I

    更新日期:1998-08-01 00:00:00

  • Pro-oxidant-mediated hepatic fibrosis and effects of antioxidant intervention in murine dietary steatohepatitis.

    abstract::The mechanistic significance of oxidative stress to fibrogenesis in the methionine and choline-deficient (MCD) diet-induced model of steatohepatitis was evaluated by antioxidant intervention, using either vitamin E or L-2-oxothiazolidine-4-carboxylate (OTC), a cysteine precursor that promotes glutathione synthesis. Si...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Phung N,Pera N,Farrell G,Leclercq I,Hou JY,George J

    更新日期:2009-08-01 00:00:00

  • Overexpression of Her-2 upregulates FoxM1 in gastric cancer.

    abstract::The transcription factor, forkhead box protein M1 (FoxM1), and the tyrosine kinase receptor, human epidermal growth factor receptor-2 (Her-2), are aberrantly expressed in a number of human malignancies, and are closely associated with the development of cancer. However, their regulatory mechanisms and their involvemen...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Qi W,Li X,Zhang Y,Yao R,Qiu W,Tang D,Liang J

    更新日期:2014-06-01 00:00:00

  • FERM family proteins and their importance in cellular movements and wound healing (review).

    abstract::Motility is a requirement for a number of biological processes, including embryonic development, neuronal development, immune responses, cancer progression and wound healing. Specific to wound healing is the migration of endothelial cells, fibroblasts and other key cellular players into the wound space. Aberrations in...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审


    authors: Bosanquet DC,Ye L,Harding KG,Jiang WG

    更新日期:2014-07-01 00:00:00

  • Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro.

    abstract::A substantial increase in NADH production, arising from accelerated glycolysis, occurs in cardiac hypertrophy and this raises the question of how the NADH is oxidised. We have addressed this problem by reconstructing appropriate mitochondrial shuttles in vitro, using mitochondria from the left ventricles of both normo...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Atlante A,Seccia TM,De Bari L,Marra E,Passarella S

    更新日期:2006-07-01 00:00:00

  • Pro- and anti-inflammatory cytokines increase hyaluronan production by rat synovial membrane in vitro.

    abstract::Synovial membrane consists of fibroblasts and macrophages forming the synovial lining supported by vascularized subsynovium. Each of these components may specifically react to a particular stimulus. Thus, reactions of isolated synovial cells may not correspond to that of intact tissue. We characterized the production ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Hyc A,Osiecka-Iwan A,Niderla-Bielinska J,Jankowska-Steifer E,Moskalewski S

    更新日期:2009-10-01 00:00:00

  • Protective effect of human serum amyloid P on CCl4-induced acute liver injury in mice.

    abstract::Human serum amyloid P (hSAP), a member of the pentraxin family, inhibits the activation of fibrocytes in culture and inhibits experimental renal, lung, skin and cardiac fibrosis. As hepatic inflammation is one of the causes of liver fibrosis, in the present study, we investigated the hepatoprotective effects of hSAP a...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Cong M,Zhao W,Liu T,Wang P,Fan X,Zhai Q,Bao X,Zhang D,You H,Kisseleva T,Brenner DA,Jia J,Zhuang H

    更新日期:2017-08-01 00:00:00

  • MicroRNA‑16 inhibits endometrial stromal cell migration and invasion through suppression of the inhibitor of nuclear factor‑κB kinase subunit β/nuclear factor‑κB pathway.

    abstract::Accumulating evidence has demonstrated that endometrial stromal cells (ESCs) are responsible for the pathogenesis of endometriosis (Ems), which is characterized by the presence of functional endometrial‑like tissues outside the uterine cavity. Abnormal expression of microRNAs (miRNAs) in ESCs may be implicated in the ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Wang X,Ren R,Shao M,Lan J

    更新日期:2020-08-01 00:00:00

  • Roles of SEA-expressing Staphylococcus aureus, isolated from an atopic dermatitis patient, on expressions of human beta-defensin-2 and inflammatory cytokines in HaCaT cells.

    abstract::Atopic dermatitis (AD) shows an increased susceptibility to Staphylococcus aureus infection partly due to decreased expression of human beta-defensin-2 (HBD-2). Interestingly, it was reported that the nasal carrier S. aureus down-regulates the expression of HBD-2 and -3, thereby the carrier strains of S. aureus retain...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Cho JW,Cho SY,Lee KS

    更新日期:2009-03-01 00:00:00

  • Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells.

    abstract::Radiotherapy and adjuvant cisplatin (DDP) chemotherapy are standard approaches used in the treatment of nasopharyngeal carcinoma (NPC). However, resistance to chemotherapy has recently become more common, resulting in the failure of this combination therapy for NPC. The aim of the present study was to assess the cellu...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhang P,Liu H,Xia F,Zhang QW,Zhang YY,Zhao Q,Chao ZH,Jiang ZW,Jiang CC

    更新日期:2014-01-01 00:00:00

  • miR‑195 promotes LPS‑mediated intestinal epithelial cell apoptosis via targeting SIRT1/eIF2a.

    abstract::A microarray analysis of an animal model with experimental sepsis induced by caecal ligation and puncture revealed that the level of microRNA‑195 (miR‑195) was upregulated. However, to the best of our knowledge, the role of miR‑195 in sepsis remains unknown. The present study investigated the effect of miR‑195 on apop...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Yuan T,Zhang L,Yao S,Deng SY,Liu JQ

    更新日期:2020-02-01 00:00:00

  • Bone morphogenetic protein-2 induces hypophosphorylation of Rb protein and repression of E2F in androgen-treated LNCaP human prostate cancer cells.

    abstract::Bone morphogenetic protein-2 (BMP-2), a multifunctional member of the transforming growth factor (TGF)-beta, superfamily, has powerful osteoinductive effects and causes cell cycle arrest in a variety of transformed cell lines. We have observed BMP-2-induced inhibition of cell proliferation in an androgen-dependent hum...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Tomari K,Kumagai T,Shimizu T,Takeda K

    更新日期:2005-02-01 00:00:00

  • Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model.

    abstract::Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sativa L.) was reporte...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Han M,Bae JS,Ban JJ,Shin HS,Lee DH,Chung JH

    更新日期:2018-05-01 00:00:00

  • CpG methylation of the ERalpha and ERbeta genes in breast cancer.

    abstract::Aberrant methylation of promoter CpG islands is known to be responsible for the alteration and silencing of genes in cancer. The data presented here describes that the CpG methylations of ERalpha and ERbeta are found in breast cancer tissues, and methylation exerts a considerable effect on gene silencing, investigated...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Kim SJ,Kim TW,Lee SY,Park SJ,Kim HS,Chung KW,Lee ES,Kang HS

    更新日期:2004-08-01 00:00:00

  • Induction of apoptosis by S-allylmercapto-L-cysteine, a biotransformed garlic derivative, on a human gastric cancer cell line.

    abstract::Epidemiological and experimental carcinogenesis studies provide evidence that certain components of garlic have anti-cancer activity. Although the biotransformed garlic derivative S-allylmercapto-L-cysteine (SAMC) has been reported to show an inhibitory effect on tumorigenesis, the mechanisms are poorly understood. Th...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Lee Y

    更新日期:2008-06-01 00:00:00

  • The cytoprotective role of gemcitabine-induced autophagy associated with apoptosis inhibition in triple-negative MDA-MB-231 breast cancer cells.

    abstract::Triple-negative breast cancer (TNBC), which is estrogen receptor (ER)-negative, progesterone receptor‑negative and is also negative for HER2 expression, remains a great clinical challenge due to its strong resistance to chemotherapy at the late stage of treatment and relatively unfavorable prognosis. Gemcitabine has b...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Chen M,He M,Song Y,Chen L,Xiao P,Wan X,Dai F,Shen P

    更新日期:2014-07-01 00:00:00

  • Effects of grape seed proanthocyanidin extract on pentylenetetrazole-induced kindling and associated cognitive impairment in rats.

    abstract::Numerous studies have demonstrated the antioxidant effects of grape seed proanthocyanidin extract (GSPE). The generation of free radicals and the ensuing apoptosis may contribute to the pathogenesis of epilepsy; therefore, in the present study, we examined the effects of GSPE on cognitive impairment and neuronal damag...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhen J,Qu Z,Fang H,Fu L,Wu Y,Wang H,Zang H,Wang W

    更新日期:2014-08-01 00:00:00

  • Hirschsprung, RET-SOX and beyond: the challenge of examining non-mendelian traits (Review).

    abstract::Hirschsprung disease (HSCR), or congenital intestinal aganglionosis, is a common hereditary disorder causing intestinal obstruction, thereby showing considerable phenotypic variation in conjunction with complex inheritance. Moreover, phenotypic assessment of the disease has been complicated since a subset of the obser...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审


    authors: Pusch CM,Sasiadek MM,Blin N

    更新日期:2002-10-01 00:00:00

  • Tetramethylpyrazine reduces inflammation levels and the apoptosis of LPS‑stimulated human periodontal ligament cells via the downregulation of miR‑302b.

    abstract::Periodontitis is the main cause of tooth or tissue loss. Human periodontal ligament stem cells (hPDLSCs), which have high proliferative, self‑renewal and multi‑differentiation abilities, are vital for the restoration of periodontitis‑induced injuries. The anti‑inflammatory and anti‑apoptotic agent, tetramethylpyrazine...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Duan Y,An W,Wu Y,Wang J

    更新日期:2020-06-01 00:00:00

  • Cloning of a rat-specific long PCP4/PEP19 isoform.

    abstract::We report the identification of a cDNA that encodes a putative protein of 94 amino acids and expected molecular weight of 10.7 kDa, the C-terminal half of which is identical to that of PEP19, a small, brain-specific protein involved in Ca++/calmodulin signaling. The novel rat-specific protein, tentatively named long P...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Sala A,Scaturro M,Proia P,Schiera G,Balistreri E,Aflalo-Rattenbach R,Créau N,Di Liegro I

    更新日期:2007-03-01 00:00:00

  • Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer.

    abstract::To date, only a few studies have suggested that human embryonic stem cells (hESCs) might effectively immunize against colon and lung cancer. The purpose of this study was to investigate the therapeutic potential of hESCs as a vaccine to induce widespread antitumor effects in differe...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhang Z,Chen X,Chang X,Ye X,Li Y,Cui H

    更新日期:2013-01-01 00:00:00

  • Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro.

    abstract::Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermor...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Mateu R,Živicová V,Krejčí ED,Grim M,Strnad H,Vlček Č,Kolář M,Lacina L,Gál P,Borský J,Smetana K Jr,Dvořánková B

    更新日期:2016-10-01 00:00:00

  • Exendin-4 protects bone marrow-derived mesenchymal stem cells against oxygen/glucose and serum deprivation-induced apoptosis through the activation of the cAMP/PKA signaling pathway and the attenuation of ER stress.

    abstract::Exendin-4 (ex-4) is a long-acting glucagon-like peptide-1 receptor (GLP-1R) agonist which exerts beneficial effects on glycemic control and promotes cell viability. In the present study, we investigated the anti-apoptotic effects of ex-4, as well as the potential mechanisms responsible for these effects in rat bone ma...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: He J,Wang C,Sun Y,Lu B,Cui J,Dong N,Zhang M,Liu Y,Yu B

    更新日期:2016-04-01 00:00:00

  • Propofol‑induced HOXA11‑AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR‑4458.

    abstract::Propofol is a commonly used drug for the induction and maintenance of anesthesia. Previous studies have reported that propofol is involved in the progression of numerous human cancer types, including hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms in HCC are yet to be elucidated. The prese...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Song F,Liu J,Feng Y,Jin Y

    更新日期:2020-09-01 00:00:00

  • The sphingosine-1-phosphate receptor agonist FTY720 and its phosphorylated form affect the function of CD4+CD25+ T cells in vitro.

    abstract::The sphingosine-1-phosphate receptor agonist FTY720 and FTY720-P have a wide variety of fundamental functions. Many studies have demonstrated that CD4+CD25+ regulatory T (Treg) cells engage in the maintenance of immunological self-tolerance by actively suppressing self-reactive lymphocytes. Although FTY720 has also re...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Liu Y,Jiang J,Xiao H,Wang X,Li Y,Gong Y,Huang Y

    更新日期:2012-07-01 00:00:00

  • Alleviation of asthma-related symptoms by a derivative of L-allo threonine.

    abstract::Chronic asthma is characterized by inflammatory cell infiltration and tissue remodeling, leading to subepithelial inflammation. In order to evaluate the anti-asthmatic activity of LX519290, a derivative of L-allo threonine, we performed several in vitro and in vivo anti-asthmatic assays. Using ovalbumin (OVA)-sensitiz...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Heo JC,Lee SH

    更新日期:2013-04-01 00:00:00

  • Adenosine and the adenosine A2A receptor agonist, CGS21680, upregulate CD39 and CD73 expression through E2F-1 and CREB in regulatory T cells isolated from septic mice.

    abstract::The number of regulatory T cells (Treg cells) and the expression of ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1; also known as CD39) and 5'-ectonucleotidase (NT5E; also known as CD73) on the Treg cell surface are increased during sepsis. In this study, to determine the factors leading to the high expressi...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Bao R,Shui X,Hou J,Li J,Deng X,Zhu X,Yang T

    更新日期:2016-09-01 00:00:00

  • Human immunodeficiency virus type-1 transcription: role of the 5'-untranslated leader region (review).

    abstract::Human immunodeficiency virus type-1 (HIV-1) transcription is dependent on the interaction of host-cell transcription factors with cis-regulatory DNA elements within the viral long terminal repeat (LTR). Much attention has focused on the series of sequence elements upstream of the transcriptional initiation site in the...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审


    authors: Al-Harthi L,Roebuck KA

    更新日期:1998-05-01 00:00:00

  • miR‑93‑5p regulates the occurrence and development of esophageal carcinoma epithelial cells by targeting TGFβR2.

    abstract::Emerging studies have indicated that the dysregulation of microRNAs (miRNAs or miRs) plays a vital role in the development and metastasis of tumors. However, the role of miR‑93‑5p in esophageal carcinoma (EC) has not been extensively reported. The present study thus focused on the role of miR‑93‑5p and its downstream ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Cai Y,Ruan W,Ding J,Wei N,Wang J,Zhang H,Ma N,Weng G,Su WK,Lin Y,Zhu K

    更新日期:2021-03-01 00:00:00

  • Oxidant-induced cardiomyocyte injury: identification of the cytoprotective effect of a dopamine 1 receptor agonist using a cell-based high-throughput assay.

    abstract::Myocyte injury due to myocardial reperfusion injury plays a crucial role in the pathogenesis of acute myocardial infarction even after successful coronary revascularization. Identification of compounds that reduce reperfusion-associated myocyte death is important. Therefore, we developed an in vitro model of myocardia...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Gerö D,Módis K,Nagy N,Szoleczky P,Tóth ZD,Dormán G,Szabó C

    更新日期:2007-11-01 00:00:00