Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia.

Abstract:

:Hyposalivation is an intractable side‑effect of radiotherapy for head and neck cancer. It is caused by the irreversible loss of acinar cells and decreased saliva secretion. However, this situation severely compromises the quality of life of affected patients. Currently, there is no effective treatment for this condition. In the present study, we developed a novel approach to regenerate the function of the irradiation‑damaged salivary glands using human adipose tissue‑derived stem cell (hADSC) intraglandular transplantation. ZsGreen‑labeled hADSCs were adoptively transferred into Sprague‑Dawley (SD) rat submandibular glands immediately following exposure to 18 Gy irradiation. A higher salivary flow rate (SFR) was observed in the hADSC‑treated group. Tissue improvement, including angiogenesis, anti‑apoptosis and anti‑fibrosis, was detected in the hADSC‑treated glands as compared to the untreated glands. Quantitative reverse transcription PCR (RT-qPCR) revealed a significantly higher expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), cyclooxygenase‑2 (COX‑2) and matrix metalloproteinase‑2 (MMP‑2) in the hADSC‑treated rats. Furthermore, immunohistochemical analysis indicated that the hADSCs had differentiated into acinar and ductal cells in the rat submandibular glands. Thus, our results suggest that hADSCs are able to regenerate irradiation‑damaged salivary glands through glandular transplantation.

journal_name

Int J Mol Med

authors

Xiong X,Shi X,Chen F

doi

10.3892/ijmm.2014.1837

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

749-55

issue

3

eissn

1107-3756

issn

1791-244X

journal_volume

34

pub_type

杂志文章
  • Profiling of alternative polyadenylation sites in luminal B breast cancer using the SAPAS method.

    abstract::Breast cancer (BC) is a leading cause of cancer-related mortality in females and is recognized as a molecularly heterogeneous disease. Previous studies have suggested that alternative messenger RNA (mRNA) processing, particularly alternative polyadenylation [poly(A)] (APA), can be a powerful molecular biomarker with p...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2014.1973

    authors: Wang X,Li M,Yin Y,Li L,Tao Y,Chen D,Li J,Han H,Hou Z,Zhang B,Wang X,Ding Y,Cui H,Zhang H

    更新日期:2015-01-01 00:00:00

  • Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening.

    abstract::The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat prot...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm_00000248

    authors: Gnanasekar M,Suleman FG,Ramaswamy K,Caldwell JD

    更新日期:2009-10-01 00:00:00

  • TGF-β1 secreted by M2 phenotype macrophages enhances the stemness and migration of glioma cells via the SMAD2/3 signalling pathway.

    abstract::The positive correlation between the number of M2 phenotype TAMs (M2‑TAMs) and tumour development suggests a supportive role of M2‑TAMs in glioma progression. In the present study, the molecular link between glioma cells and M2‑TAMs was investigated and it was demonstrated that transforming growth factor‑β1 (TGF‑β1) s...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2018.3923

    authors: Liu Z,Kuang W,Zhou Q,Zhang Y

    更新日期:2018-12-01 00:00:00

  • Cytokine-induced neutrophil accumulation in the pathogenesis of acute reflux esophagitis in rats.

    abstract::Although recent reports indicate an increasing incidence of patients with reflux esophagitis, its pathomechanism remains unclear. Cytokines and neutrophils, the latter of which produce reactive oxygen species (ROS), have been implicated in the formation of gastrointestinal diseases. This study investigated the roles o...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Yamaguchi T,Yoshida N,Tomatsuri N,Takayama R,Katada K,Takagi T,Ichikawa H,Naito Y,Okanoue T,Yoshikawa T

    更新日期:2005-07-01 00:00:00

  • Gap junctional communication and the tyrosine phosphorylation of connexin 43 in interaction between breast cancer and endothelial cells.

    abstract::Abnormal gap junction communication (GJC) has been associated with carcinogenesis. We investigated the role of endothelial cell GJC and connexin 43 (Cx43), the main gap junction protein in these cells, during tumour cell extravasation. GJC was determined by the ability of cells to transfer Lucifer yellow, to neighbour...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Cai J,Jiang WG,Mansel RE

    更新日期:1998-01-01 00:00:00

  • The sap of Acer okamotoanum decreases serum alcohol levels after acute ethanol ingestion in rats.

    abstract::In the present study, we examined whether Acer okamotoanum (A. okamotoanum) sap decreased the serum alcohol and acetaldehyde levels after acute ethanol treatment in a rat model. Male rats were orally administered 25, 50 or 100% A. okamotoanum sap 30 min prior to oral challenge with 3 ml of ethanol (15 ml/kg of a 20% e...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2011.724

    authors: Yoo YM,Jung EM,Kang HY,Choi IG,Choi KC,Jeung EB

    更新日期:2011-10-01 00:00:00

  • Transplacental effects of maternal feeding with high fat diets on lipid exchange and response of the splenic lymphoid system in mice offspring exposed to low doses of carcinogen.

    abstract::We studied whether feeding pregnant female mice with different fats affects lipid exchange and activity of the splenic lymphoid system in offspring exposed to low doses of carcinogen. Female mice were fed diets with either 7% or 15% corn oil or olive oil. The 4-week-old offspring of these mice were transferred to a ch...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.6.3.337

    authors: Zusman I,Ben-Hur H,Budovsky A,Geva D,Gurevich P,Tendler Y,Lavee S,Stark A,Madar Z

    更新日期:2000-09-01 00:00:00

  • Ubiquitous p63 expression in human esophageal squamous cell carcinoma.

    abstract::p63, a recently identified member of the p53 gene family, plays an important role in human tissue functions. We examined the pattern of p63 expression in human esophageal squamous cell carcinomas including early-stage cancers, and its clinicopathological significance. Immunoreactivity for p63 was detected in 96.9% (63...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Hara T,Kijima H,Yamamoto S,Kenmochi T,Kise Y,Tanaka H,Chino O,Shimada H,Takazawa K,Tanaka M,Inokuchi S,Makuuchi H

    更新日期:2004-08-01 00:00:00

  • Thrombospondin-1 induces apoptosis in primary leukemia and cell lines mediated by CD36 and Caspase-3.

    abstract::Thrombospondin-1 (TSP-1) is a naturally occurring anti-angiogenic compound that induces apoptosis of endothelial and cancer cells via its receptor CD36. The objectives of our study were to investigate the in vitro effects of TSP-1 on the apoptosis of primary human leukemia cells as well as leukemia cell lines and the ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Li K,Yang M,Yuen PM,Chik KW,Li CK,Shing MM,Lam HK,Fok TF

    更新日期:2003-12-01 00:00:00

  • Effect of soy sauce on the antioxidative capacity of the gelatin gel food 'Nikogori' measured using the chemiluminescence method.

    abstract::Oxygen-related free radicals have been suggested as a cause of aging and various diseases, for example, various cancers and rheumatoid arthritis. Because of this a radical scavenger as an antioxidant has been sought in many foods. 'Nikogori' gelatin gel from cooked fish is a traditional food in Japan. Recently, 'Nikog...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Nagatsuka N,Harada K,Ando M,Nagao K

    更新日期:2005-09-01 00:00:00

  • Involvement of endoplasmic reticulum stress-associated apoptosis in a heart failure model induced by chronic myocardial ischemia.

    abstract::Apoptosis plays a critical role in the pathogenesis of chronic myocardial ischemia (CMI) and heart failure (HF). Endoplasmic reticulum stress (ERS) is one of the newly defined signaling pathways which initiate apoptosis. Previous studies have shown that ERS-associated apoptosis is involved in the pathogenesis of HF in...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2011.612

    authors: Xin W,Li X,Lu X,Niu K,Cai J

    更新日期:2011-04-01 00:00:00

  • Flap endonuclease 1 is a promising candidate biomarker in gastric cancer and is involved in cell proliferation and apoptosis.

    abstract::As a DNA repair protein, flap endonuclease 1 (FEN1), a structure-specific 5' nuclease, plays pivotal roles in the maturation of Okazaki fragments, long-patch base excision repair, restarting of stalled replication forks and telomere maintenance. FEN1 possesses 5' endonuclease, 5' exonuclease and gap-endonuclease activ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2014.1682

    authors: Wang K,Xie C,Chen D

    更新日期:2014-05-01 00:00:00

  • Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro.

    abstract::Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermor...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2016.2706

    authors: Mateu R,Živicová V,Krejčí ED,Grim M,Strnad H,Vlček Č,Kolář M,Lacina L,Gál P,Borský J,Smetana K Jr,Dvořánková B

    更新日期:2016-10-01 00:00:00

  • Antiangiogenic activity of a concentrated effective microorganism fermentation extract.

    abstract::We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that u...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Chui CH,Gambari R,Lau FY,Hau DK,Wong RS,Cheng GY,Kok SH,Higa T,Ke B,Chan AS,Fong DW,Tang JC

    更新日期:2006-11-01 00:00:00

  • Regulation of CREB-mediated transcription by association of CDK4 binding protein p34SEI-1 with CBP.

    abstract::CREB binding protein (CBP) plays a central role in cell differentiation and proliferation, interacting with a large number of nuclear factors. To find novel nuclear factors associating with CBP, we have carried out yeast two-hybrid screening of human chondrocyte cDNA library using the C/H3 region of CBP as a bait and ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Hirose T,Fujii R,Nakamura H,Aratani S,Fujita H,Nakazawa M,Nakamura K,Nishioka K,Nakajima T

    更新日期:2003-06-01 00:00:00

  • Transcriptome analysis of Klf15‑mediated inhibitory functions in a mouse deep venous thrombosis model.

    abstract::Krüppel‑like family (KLF) members are important regulators of proinflammatory activation in the vasculature. A transcriptome study involving RNA sequencing (RNA‑seq) and quantitative PCR (qPCR) was performed to investigate Klf15 and Klf15‑regulated gene levels in C57BL/6 mice with inferior vena cava thrombi and in con...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2020.4538

    authors: Zhou J,Zhao X,Xie S,Zhou R

    更新日期:2020-06-01 00:00:00

  • Migration of mesothelioma cells correlates with histotype-specific synthesis of extracellular matrix.

    abstract::Three human pleural malignant mesothelioma cell cultures (MM) of epithelioid (E1), fibrous (F1) and byphasic (B1) histotype were studied in their synthesis of the extracellular matrix (ECM) components laminin (LM), fibronectin (FN), type IV collagen (cIV), and in their chemotactic and haptotactic migration towards the...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.4.1.67

    authors: Scarpa S,Giuffrida A,Fazi M,Coletti A,Palumbo C,Pass HI,Procopio A,Modesti A

    更新日期:1999-07-01 00:00:00

  • Effects of different cellulose membranes regenerated from Styela clava tunics on wound healing.

    abstract::The aim of this study was to investigate the therapeutic effects of three different cellulose membranes (CMs) manufactured from Styela clava tunics (SCTs) on the healing of cutaneous wounds. We examined the physical properties and therapeutic effects of three CMs regenerated from SCTs (referred to as SCT‑ CMs), includ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2017.2923

    authors: Song SH,Seong KY,Kim JE,Go J,Koh EK,Sung JE,Son HJ,Jung YJ,Kim HS,Hong JT,Hwang DY

    更新日期:2017-05-01 00:00:00

  • Characterization of the nuclear localization signal in the DNA helicase responsible for Bloom syndrome.

    abstract::Bloom syndrome (BS) is a rare genetic disorder characterized by small body size, photosensitivity, immunodeficiency and a high predisposition to various types of cancer. BLM was identified as the causative gene for BS. The BLM protein is homologous to DNA helicase and has two basic amino acid clusters in its C-termina...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.5.5.477

    authors: Hayakawa S,Kaneko H,Fukao T,Kasahara K,Matsumoto T,Furuichi Y,Kondo N

    更新日期:2000-05-01 00:00:00

  • The suitability of DNA extracted from formalin-fixed, paraffin-embedded tissues for double differential polymerase chain reaction analysis.

    abstract::Formalin-fixed, paraffin-embedded (FFPE) tissues are one of the popular sources of diagnostic materials, the easiest to store and transport. They are often used as the source of nucleic acids for retrospective molecular analyses based on DNA amplification by polymerase chain reaction (PCR). However, it is known that n...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.8.5.573

    authors: Bielawski K,Zaczek A,Lisowska U,Dybikowska A,Kowalska A,Falkiewicz B

    更新日期:2001-11-01 00:00:00

  • Chrysobalanus icaco L. extract for antiangiogenic potential observation.

    abstract::Angiogenesis is an important process in several physiological situations and it is also implicated in the development of some diseases such as diabetes and cancer. This study investigated the antiangiogenic potential of Chrysobalanus icaco methanol extract in the chicken embrionary tissue. Clinical trials for cancer t...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.5.6.667

    authors: Alves De Paulo S,Teruszkin Balassiano I,Henriques Silva N,Oliveira Castilho R,Coelho Kaplan MA,Currie Cabral M,da Costa Carvalho MG

    更新日期:2000-06-01 00:00:00

  • Bcl-2- and CrmA-inhibitable dephosphorylation and cleavage of retinoblastoma protein during etoposide-induced apoptosis.

    abstract::Cell numbers are regulated by a balance between proliferation and apoptosis (programmed cell death). Recent evidence suggests that proteins regulating cell proliferation also mediate apoptosis. Therefore, cellular fate might be determined by cross talk between regulators of cell cycle progression and apoptosis. Previo...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.1.1.131

    authors: An B,Johnson DE,Jin JR,Antoku K,Dou QP

    更新日期:1998-01-01 00:00:00

  • Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics.

    abstract::In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low m...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2018.3760

    authors: Righi V,Constantinou C,Kesarwani M,Rahme LG,Tzika AA

    更新日期:2018-10-01 00:00:00

  • Differential expression of genes during TPA-induced differentiation of human prostatic cancer TSU-Pr1 cells.

    abstract::We are interested in the possibility of a new prostate cancer therapy that would control tumor malignancy via induction of terminal cell differentiation. We previously reported that 12-O-tetra-decanoylphorbol-13-acetate (TPA) induces differentiation into cells with characteristics of microglia and decreases the malign...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Suzuki T,Shimizu T,Satoh K,Sugibayashi R,Takeda K

    更新日期:2002-12-01 00:00:00

  • Significance of heme oxygenase in prolactin-mediated cell proliferation and angiogenesis in human endothelial cells.

    abstract::Our objectives were to determine whether heme oxygenase-1 is a second messenger for prolactin-mediated angiogenesis. Endothelial cell proliferation and angiogenesis assay demonstrated that cell number and capillary formation were increased by prolactin (10 and 25 ng/ml). Both protein synthesis and mRNA analysis confir...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Malaguarnera L,Pilastro MR,Quan S,Ghattas MH,Yang L,Mezentsev AV,Kushida T,Abraham NG,Kappas A

    更新日期:2002-10-01 00:00:00

  • The miR-200 family regulates the epithelial-mesenchymal transition induced by EGF/EGFR in anaplastic thyroid cancer cells.

    abstract::The miR-200 family was recently identified as a suppressor of epithelial-mesenchymal transition (EMT). The loss or gain of miR-200 family members is associated with cancer invasion. The epidermal growth factor receptor (EGFR) is overexpressed in the majority of anaplastic thyroid cancers (ATCs). The activation of EGFR...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2012.1059

    authors: Zhang Z,Liu ZB,Ren WM,Ye XG,Zhang YY

    更新日期:2012-10-01 00:00:00

  • Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis.

    abstract::Imatinib is the therapeutic standard for newly diagnosed patients with chronic myeloid leukemia (CML). In these patients, imatinib has been shown to induce an apoptotic response specifically in cells expressing the oncogenic fusion protein BCR-ABL. Previous studies in our lab revealed that imatinib-induced apoptosis i...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2011.872

    authors: Liu J,Xiao Y,Xiong HM,Li J,Huang B,Zhang HB,Feng DQ,Chen XM,Wang XZ

    更新日期:2012-04-01 00:00:00

  • Increased expression of human histocompatibility leukocyte antigen-G in colorectal cancer cells.

    abstract::Human histocompatibility leukocyte antigen (HLA)-G is a nonclassical major histocompatibility complex class I molecule. HLA-G is known to provide tolerance from recognition by natural killer cells. We studied HLA-G expression in 39 human colorectal cancers and 23 extra-neoplastic colon tissue samples by RT-PCR. The ex...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2.3.349

    authors: Fukushima Y,Oshika Y,Nakamura M,Tokunaga T,Hatanaka H,Abe Y,Yamazaki H,Kijima H,Ueyama Y,Tamaoki N

    更新日期:1998-09-01 00:00:00

  • Telomerase activity as a novel marker of lung cancer and immune-associated lung diseases.

    abstract::Telomerase maintains telomere length and is considered to be necessary for the indefinite proliferation of human cells. Telomerase activity is detected not only in germline and immortal cancer cells, but also in stem/progenitor cells of renewal tissues and activated lymphocytes. While it is generally agreed that telom...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Hiyama K,Ishioka S,Shay JW,Taooka Y,Maeda A,Isobe T,Hiyama E,Maeda H,Yamakido M

    更新日期:1998-03-01 00:00:00

  • Clinical significance and molecular mechanisms of bioinactive growth hormone (review).

    abstract::About 80% of short children are not deficient in endogenous growth hormone (GH) and termed idiopathic short stature (ISS). The causes of impaired growth in children with ISS are various. Short stature and low insulin-like growth factor-I (IGF-I) concentration despite normal to high GH concentration suggest impaired GH...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审

    doi:10.3892/ijmm.2.3.287

    authors: Takahashi Y,Chihara K

    更新日期:1998-09-01 00:00:00