Abstract:
:We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demonstrated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Chui CH,Gambari R,Lau FY,Hau DK,Wong RS,Cheng GY,Kok SH,Higa T,Ke B,Chan AS,Fong DW,Tang JCsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
975-9issue
5eissn
1107-3756issn
1791-244Xjournal_volume
18pub_type
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