AMP-activated protein kinase regulates thyroid hormone-stimulated osteocalcin synthesis in osteoblasts.

Abstract:

:AMP-activated protein kinase (AMPK) is recognized as a main regulator of energy homeostasis. Osteocalcin (OC), which is produced specifically by mature osteoblasts, is stored in bone matrix, strongly binds to hydroxyapatite and is released into the circulation, has been recognized as a marker of bone metabolism. It has recently been shown that OC released from osteoblasts influences energy metabolism as a hormone. We previously reported that triiodothyronine (T3) stimulates the synthesis of OC in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether AMPK participates in T3-stimulated OC synthesis in osteoblasts. T3 time-dependently induced the phosphorylation of the AMPKα-subunit (Thr-172), whereas T3 failed to induce the phosphorylation of AMPKα-subunit (Ser-485), AMPKβ-subunit (Ser-108) and AMPKβ-subunit (Ser-182). Both the release and the mRNA expression of OC induced by T3 were significantly inhibited by compound C, an AMPK inhibitor. Compound C suppressed the T3-induced phosphorylation of acetyl-CoA carboxylase, a direct substrate of AMPK. T3-stimulated OC release was significantly reduced in AMPK-knockdown cells using AMPK-siRNA. These results strongly suggest that AMPK positively regulates T3-stimulated OC synthesis in osteoblasts.

journal_name

Int J Mol Med

authors

Kondo A,Otsuka T,Kato K,Matsushima-Nishiwaki R,Kuroyanagi G,Mizutani J,Tokuda H,Kozawa O

doi

10.3892/ijmm.2013.1349

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

1457-62

issue

6

eissn

1107-3756

issn

1791-244X

journal_volume

31

pub_type

杂志文章
  • Analysis of boutique arrays: a universal method for the selection of the optimal data normalization procedure.

    abstract::DNA microarrays, which are among the most popular genomic tools, are widely applied in biology and medicine. Boutique arrays, which are small, spotted, dedicated microarrays, constitute an inexpensive alternative to whole-genome screening methods. The data extracted from each microarray-based experiment must be transf...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2013.1443

    authors: Uszczyńska B,Zyprych-Walczak J,Handschuh L,Szabelska A,Kaźmierczak M,Woronowicz W,Kozłowski P,Sikorski MM,Komarnicki M,Siatkowski I,Figlerowicz M

    更新日期:2013-09-01 00:00:00

  • COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer.

    abstract::Cyclooxygenase-2 (COX-2) participates in cancer invasion and metastasis by decreasing the expression of E-cadherin. However, the molecular mechanisms through which COX-2 regulates E-cadherin expression and function have not yet been fully elucidated. The aim of this study was to investigate the possible molecular mech...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2013.1376

    authors: Chen Z,Liu M,Liu X,Huang S,Li L,Song B,Li H,Ren Q,Hu Z,Zhou Y,Qiao L

    更新日期:2013-07-01 00:00:00

  • Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

    abstract::The purpose of the present study was to investigate whether bone marrow mesenchymal stem cells (BMMSCs) modified by CXC‑chemokine receptor type 3 (CXCR3) and heme oxygenase‑1 (HO‑1) genes can repair damaged intestinal epithelial cells in vitro, and the role of the p38 mitogen‑activated protein kinase (p38‑MAPK) pathwa...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2019.4120

    authors: Yin M,Shen Z,Yang L,Zheng W,Song H

    更新日期:2019-05-01 00:00:00

  • Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions.

    abstract::Excessive expansion of the transit-amplifying (TA) cell compartment is a distinct morphological characteristic of psoriatic epidermal hyperplasia. In order to examine the activation of basal stem cells and how they replenish such an enlarged compartment of TA cells in psoriatic epidermis, we utilized a BrdU labeling m...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2015.2445

    authors: Jia HY,Shi Y,Luo LF,Jiang G,Zhou Q,Xu SZ,Lei TC

    更新日期:2016-02-01 00:00:00

  • Possible protective role of the ABCA4 gene c.1268A>G missense variant in Stargardt disease and syndromic retinitis pigmentosa in a Sicilian family: Preliminary data.

    abstract::In the wide horizon of ophthalmologically rare diseases among retinitis pigmentosa forms, Stargardt disease has gradually assumed a significant role due to its heterogeneity. In the present study, we aimed to support one of two opposite hypotheses concerning the causative or protective role of heterozygous c.1268A>G m...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2017.2917

    authors: D'Angelo R,Donato L,Venza I,Scimone C,Aragona P,Sidoti A

    更新日期:2017-04-01 00:00:00

  • Evaluation of the anti-allergic activity of Citrus unshiu using rat basophilic leukemia RBL-2H3 cells as well as basophils of patients with seasonal allergic rhinitis to pollen.

    abstract::The anti-allergic activity of the 50% methanol extract of Citrus unshiu powder (MEC) was examined. Fifty percent methanol extract of MEC powder showed potent inhibitory activity against histamine release from basophils of patients suffering from seasonal allergic rhinitis to ceder pollen. To examine this anti-allergic...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Kobayashi S,Tanabe S

    更新日期:2006-03-01 00:00:00

  • Transplacental effects of maternal feeding with high fat diets on lipid exchange and response of the splenic lymphoid system in mice offspring exposed to low doses of carcinogen.

    abstract::We studied whether feeding pregnant female mice with different fats affects lipid exchange and activity of the splenic lymphoid system in offspring exposed to low doses of carcinogen. Female mice were fed diets with either 7% or 15% corn oil or olive oil. The 4-week-old offspring of these mice were transferred to a ch...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.6.3.337

    authors: Zusman I,Ben-Hur H,Budovsky A,Geva D,Gurevich P,Tendler Y,Lavee S,Stark A,Madar Z

    更新日期:2000-09-01 00:00:00

  • Exogenous H2S mitigates myocardial fibrosis in diabetic rats through suppression of the canonical Wnt pathway.

    abstract::Hydrogen sulfide (H2S) has antifibrotic activity in the kidneys, heart, lungs, and other organs. The present study investigated the protective activity of exogenous H2S against myocardial fibrosis in a rat model of diabetes. Animals were assigned to normal control, diabetes mellitus (DM), DM + sodium hydrosulfide (NaH...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2019.4237

    authors: Yang R,Jia Q,Ma SF,Wang Y,Mehmood S,Chen Y

    更新日期:2019-08-01 00:00:00

  • Sevoflurane anesthesia persistently downregulates muscle-specific microRNAs in rat plasma.

    abstract::The volatile anesthetic, sevoflurane, is widely used in surgery. Over the years, there has been a growing interest in the biological effects of sevoflurane on tissue and organ systems and the molecular mechanisms involved. MicroRNAs (miRNAs or miRs) acting as pivotal post‑transcriptional regulators for fine-tuning gen...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2014.1739

    authors: Takeuchi J,Sakamoto A,Takizawa T

    更新日期:2014-07-01 00:00:00

  • Prevalence of the C282Y and H63D polymorphisms in a multi-ethnic control population.

    abstract::Recently a candidate gene for hereditary hemo-chromatosis, HFE, was identified. The finding raises the possibility for genetic testing to provide earlier detection and more complete genotypic evaluation of hemochromatosis affected individuals. We determined the frequency of the HFE polymorphisms, C282Y and H63D, in a ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.4.4.389

    authors: Marshall DS,Linfert DR,Tsongalis GJ

    更新日期:1999-10-01 00:00:00

  • Luteolin inhibits multi-heavy metal mixture-induced HL7702 cell apoptosis through downregulation of ROS-activated mitochondrial pathway.

    abstract::With the rapid economic development in recent years, China is facing a great challenge due to heavy metal pollution. The heavy metals may enter the human body through ingestion of aqua products to cause great health risks. In the present study, the inhibitory effects of luteolin on the combined toxicity of multi-heavy...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2017.3219

    authors: Wang Y,Su H,Song X,Fiati Kenston SS,Zhao J,Gu Y

    更新日期:2018-01-01 00:00:00

  • Regulation of vascular smooth muscle proliferation and migration by beta2-chimaerin, a non-protein kinase C phorbol ester receptor.

    abstract::The proliferation and migration of vascular smooth muscle cells (SMC) are important aspects of atherogenesis. Activated growth factor signaling in injured vessels subsequently promotes a number of intracellular events resulting in the phenotypic modulation of SMC. Here, we investigated the role of beta2-chimaerin, a n...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Maeda M,Kato S,Fukushima S,Kaneyuki U,Fujii T,Kazanietz MG,Oshima K,Shigemori M

    更新日期:2006-04-01 00:00:00

  • (1)H nuclear magnetic resonance metabolomic analysis of mammary tumors from lean and obese Zucker rats exposed to 7,12-dimethylbenz[a]anthracene.

    abstract::Obesity increases mammary tumor development in Zucker rats following a single administration of the procarcinogen 7,12-dimenthylbenz(a)anthracene (DMBA). Fifty-day-old obese and lean female Zucker rats were orally gavaged with 65 mg/kg DMBA and sacrificed 139 days post DMBA treatment. At the end of the experiment, mam...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Whitehead TL,Holley AW,Korourian S,Shaaf S,Kieber-Emmons T,Hakkak R

    更新日期:2007-10-01 00:00:00

  • Tumor necrosis factor-α acts reciprocally with solute carrier family 26, member 3, (downregulated-in-adenoma) and reduces its expression, leading to intestinal inflammation.

    abstract::Solute carrier family 26, member 3 (Slc26a3), also termed downregulated-in-adenoma (DRA) is a member of the Slc26 family of anion transporters and is mutated in congenital chloride diarrhea. Our previous study demonstrated that DRA deficiency is associated with severely reduced colonic HCO3‑ secretion, a loss of colon...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2017.3347

    authors: Ding X,Li D,Li M,Tian D,Yu H,Yu Q

    更新日期:2018-03-01 00:00:00

  • Activation of the receptor for parathyroid hormone and parathyroid hormone related protein induces apoptosis via the extrinsic and intrinsic signaling pathway.

    abstract::Parathyroid hormone (PTH) is the primary regulator of serum calcium homeostasis and bone metabolism. PTH acts primarily by binding to its receptor, PTH1R, in the bone and kidney. In addition to PTH, PTH1R also recognizes PTH-related peptide (PTHrP), a paracrine/autocrine factor originally described as the hormone resp...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm_00000242

    authors: Müller M,Gagiannis S,Nawroth PP,Brune M,Schilling T

    更新日期:2009-09-01 00:00:00

  • Multi‑layered prevention and treatment of chronic inflammation, organ fibrosis and cancer associated with canonical WNT/β‑catenin signaling activation (Review).

    abstract::β‑catenin/CTNNB1 is an intracellular scaffold protein that interacts with adhesion molecules (E‑cadherin/CDH1, N‑cadherin/CDH2, VE‑cadherin/CDH5 and α‑catenins), transmembrane‑type mucins (MUC1/CD227 and MUC16/CA125), signaling regulators (APC, AXIN1, AXIN2 and NHERF1/EBP50) and epigenetic or transcriptional regulator...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审

    doi:10.3892/ijmm.2018.3689

    authors: Katoh M

    更新日期:2018-08-01 00:00:00

  • Roles of oncogenes and tumor-suppressor genes in osteoclastogenesis (Review).

    abstract::Osteoporosis is a bone disease that poses a tremendous burden to health care. The receptor activator of nuclear factor-κB (RANK) and its ligand (RANKL) have been a major focus of this research field. RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审

    doi:10.3892/ijmm.2017.2847

    authors: Minami A,Ogino M,Nakano N,Ichimura M,Nakanishi A,Murai T,Kitagishi Y,Matsuda S

    更新日期:2017-02-01 00:00:00

  • EETs and CYP2J2 inhibit TNF-α-induced apoptosis in pulmonary artery endothelial cells and TGF-β1-induced migration in pulmonary artery smooth muscle cells.

    abstract::Cytochrome P450 epoxygenase-derived epoxyeicosatrienoic acids (EETs) have multiple biological functions in cardiovascular homeostasis. The antiinflammatory, anti-migratory and pro-proliferative effects of EETs suggest a possible beneficial role for EETs in the apoptosis, proliferation and migration of pulmonary vascular...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2013.1435

    authors: Feng W,Xu X,Zhao G,Li G,Liu T,Zhao J,Dong R,Wang DW,Tu L

    更新日期:2013-09-01 00:00:00

  • iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis.

    abstract::Tuberculous meningitis (TBM) is a serious complication of tuberculosis that affects the central nervous system. As TBM may result in permanent sequelae and death, rapid, accurate diagnostic tests using novel biomarkers are required for the early diagnosis and treatment of TBM. A quantitative proteomic study was theref...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2015.2131

    authors: Yang Y,Mu J,Chen G,Zhan Y,Zhong J,Wei Y,Cheng K,Qin B,You H,Xie P

    更新日期:2015-05-01 00:00:00

  • Quercetin inhibits the growth of human gastric cancer stem cells by inducing mitochondrial-dependent apoptosis through the inhibition of PI3K/Akt signaling.

    abstract::Cancer stem cells (CSCs) have recently been linked to new treatment strategies for gastric cancer due to the critical role which they play as the 'heartbeat' of cancer. In the present study, we explored the effects of quercetin, an anti-inflammatory and antiviral compound, on gastric CSCs (GCSCs). We noted that quercet...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2016.2625

    authors: Shen X,Si Y,Wang Z,Wang J,Guo Y,Zhang X

    更新日期:2016-08-01 00:00:00

  • NR2F2 loss‑of‑function mutation is responsible for congenital bicuspid aortic valve.

    abstract::Congenital bicuspid aortic valve (BAV) represents the most common type of cardiac birth defect affecting 0.4‑2% of the general population, and accounts for a markedly increased incidence of life‑threatening complications, including valvulopathy and aortopathy. Accumulating evidence has demonstrated the genetic basis o...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2019.4087

    authors: Wang J,Abhinav P,Xu YJ,Li RG,Zhang M,Qiu XB,Di RM,Qiao Q,Li XM,Huang RT,Xue S,Yang YQ

    更新日期:2019-04-01 00:00:00

  • Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth.

    abstract::This study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (BPA) strains associated with the development and progress of periodontal diseases: Porphyromonas gingivalis ATCC 33277, Prevotella i...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Fadel M,Courtois P

    更新日期:2001-07-01 00:00:00

  • Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.

    abstract::Determination of CD34+ cells was performed in bone marrow and G-CSF mobilised peripheral blood samples. We adopted three different protocols of analysis: the Milan/Mulhouse protocol, the ISHAGE guidelines for CD34+ cell determination and our own protocol based upon the use of PAINT-A-GATEPRO software analysis program....

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.1.1.67

    authors: Venditti A,Buccisano F,Del Poeta G,Battaglia A,Tamburini A,Bruno A,Adorno G,Caravita T,Santinelli S,Del Moro B,Epiceno AM,Ballatore G,Amadori S

    更新日期:1998-01-01 00:00:00

  • Apolipoprotein(a) phenotypes are reliable biomarkers for familial aggregation of coronary heart disease.

    abstract::Lipoprotein(a) [Lp(a)] is an atherogenic and prothrombotic molecule formed by the covalent binding of the highly polymorphic apolipoprotein(a) [apo(a)] to apoprotein B-100 of LDL. High Lp(a) concentrations are a recognized genetic risk factor for coronary heart disease (CHD) and have been shown to be related with a fa...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Peros E,Geroldi D,D'Angelo A,Falcone C,Montagna L,Carabela M,Emanuele E

    更新日期:2004-02-01 00:00:00

  • Neuromedin-U stimulates enucleation-induced adrenocortical regeneration in the rat.

    abstract::Neuromedin-U (NMU) is a brain-gut peptide, which has been previously found to stimulate hypothalamic-pituitary-adrenal axis in the rat. Enucleation-induced adrenal regeneration in rats with contralateral adrenalectomy is a well-established model of adrenal growth, that not only depends on the compensatory ACTH hyperse...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:

    authors: Trejter M,Neri G,Rucinski M,Majchrzak M,Nussdorfer GG,Malendowicz LK

    更新日期:2008-06-01 00:00:00

  • Proteolytic degradation of the retinoblastoma family protein p107: A putative cooperative role of calpain and proteasome.

    abstract::p107 protein, a member of the retinoblastoma family protein, suppresses growth promotion in cancer cells. We have already reported evidence that calpain, a calcium dependent protease is involved in the cleavage of p107 protein. We show here that p107 protein can also be a substrate for ubiquitination. A negative growt...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.4.5.487

    authors: Jang JS,Choi YH

    更新日期:1999-11-01 00:00:00

  • MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1.

    abstract::The aim of this study was to investigate the role of microRNAs (miRNAs or miRs) in vascular smooth muscle cell (VSMC) proliferation and to elucidate the underlying molecular mechanisms. In a previous study, using microarray analysis, differentially expressed miRNAs were identified in primary VSMCs isolated from the me...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2015.2277

    authors: Liu K,Ying Z,Qi X,Shi Y,Tang Q

    更新日期:2015-09-01 00:00:00

  • Daidzein exerts anticancer activity towards SKOV3 human ovarian cancer cells by inducing apoptosis and cell cycle arrest, and inhibiting the Raf/MEK/ERK cascade.

    abstract::Ovarian cancer is the main cause of gynecological cancer‑associated mortality around the world. Despite initial responses to chemotherapy, frequent relapse occurs. Daidzein is an important flavonoid and has been shown to exhibit a diversity of pharmacological properties, including antimicrobial and anticancer activiti...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2018.3531

    authors: Hua F,Li CH,Chen XG,Liu XP

    更新日期:2018-06-01 00:00:00

  • Vigna angularis inhibits mast cell-mediated allergic inflammation.

    abstract::The aim of the present study was to elucidate whether extracts of Vigna angularis (EVA) inhibit allergic inflammatory reactions and to elucidate the possible mechanisms of action. For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines from human mast ce...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2013.1430

    authors: Kim HH,Kim SW,Kim DS,Oh HM,Rho MC,Kim SH

    更新日期:2013-09-01 00:00:00

  • TNF-α induces Drp1-mediated mitochondrial fragmentation during inflammatory cardiomyocyte injury.

    abstract::Dynamin-related peptide 1 (Drpl)-mediated mitochondrial fission is an important process associated with cardiac dysfunction under different pathological conditions. The aim of the present study was to investigate the expression of Drpl during inflammatory myocardial injury. Sprague‑Dawley rats were treated intraperito...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章

    doi:10.3892/ijmm.2018.3385

    authors: Shen YL,Shi YZ,Chen GG,Wang LL,Zheng MZ,Jin HF,Chen YY

    更新日期:2018-04-01 00:00:00