Tau complexes with phospholipase C-gamma in situ.

Abstract:

:Based on the results of recent in vitro studies, tau has been proposed to be involved in regulating signal transduction through the phospholipase C-gamma (PLC-gamma) signaling pathway. The present study provides support for the physiological relevance of this hypothesis by demonstrating the existence of a tau-PLC-gamma complex in situ in a human neuroblastoma cell line. Both PLC-gamma and PLC-delta, but not PLC-beta, co-purified with microtubule-associated proteins. PLC-gamma, but neither PLC-delta nor PLC-beta, co-immunoprecipitated with tau, and the PLC co-precipitating with tau was enzymatically active. Additionally, both tau and MAP-2 co-precipitated with PLC-gamma. These studies indicate that tau associates, either directly or indirectly, with PLC-gamma in situ, suggesting that tau may be appropriately localized to participate in the regulation of signal transduction through the PLC-gamma pathway in vivo.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Jenkins SM,Johnson GV

doi

10.1097/00001756-199801050-00014

subject

Has Abstract

pub_date

1998-01-05 00:00:00

pages

67-71

issue

1

eissn

0959-4965

issn

1473-558X

journal_volume

9

pub_type

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