Abstract:
:The orphanin-FQ/nociceptin (OFQ/N) receptor (previously, ORL1, LC132) has been shown to be coupled to an inwardly rectifying K+ conductance in several neuronal populations. Although OFQ/N receptor mRNA is densely expressed in the supraoptic nucleus (SON), little is known about its coupling to effector system(s). The present study examined the effects of OFQ/N on guinea pig magnocellular neurons within the SON using intracellular recording from hypothalamic slices. In the presence of tetrodotoxin, OFQ/N hyperpolarized 48 of 48 SON magnocellular neurons, 24 of which were subsequently identified by immunocytochemistry as arginine vasopressin positive (AVP+). Nineteen of the 48 SON neurons, including 7 which were AVP+, responded to OFQ/N with an outward current that reversed at the K+ equilibrium potential (EK+) and a decrease in slope resistance consistent with the activation of an inwardly rectifying K+ channel. In 4 of these neurons, BaCl2 significantly attenuated both the hyperpolarization and the decrease in slope resistance induced by OFQ/N. Twenty-one SON neurons, 13 of which were AVP+, responded to OFQ/N with an increase in slope resistance which did not reverse at EK+. An additional 5 neurons (2 were AVP+) were treated with the gap junction blocking agent carbenoxolone (CARB). CARB induced a small hyperpolarization, increased slope resistance and significantly reduced the subsequent OFQ/N-induced hyperpolarization. However, when the CARB and CARB plus OFQ/N hyperpolarizations were summed in these 5 cells, they were no different than the OFQ/N hyperpolarization alone. The effect of two putative OFQ/N receptor antagonists was also evaluated. The kappa3-opioid antagonist naloxone benzoylhydrazone was without effect (n = 3), and the 13-amino-acid [Phe1Psi(CH2-NH)Gly2]OFQ/N(1-13)NH2 OFQ/N analog produced a small hyperpolarization on its own in addition to partially antagonizing the effects of OFQ/N (n = 3). Taken together, these results suggest that OFQ/N acts upon SON neurons through two mechanisms, one which hyperpolarizes the neuron by activating an inwardly rectifying K+ conductance, and another which may increase membrane resistance by closing the low-resistance gap junctions.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Slugg RM,Rønnekleiv OK,Grandy DK,Kelly MJdoi
10.1159/000054441subject
Has Abstractpub_date
1999-05-01 00:00:00pages
385-96issue
5eissn
0028-3835issn
1423-0194pii
54441journal_volume
69pub_type
杂志文章abstract::Plasma and hypothalamic LHRH was measured by specific radioimmuno-assay in intact and ovariectomized rats, and the values were correlated with peripheral plasma (PP) FSH and LH titers. At most stages of the estrous cycle, plasma LHRH was either undetectable or present at very minimal values. An increase in PP LHRH con...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122496
更新日期:1976-01-01 00:00:00
abstract::Molecules of astrocyte origin influence gonadotropin-releasing hormone (GnRH) release and GnRH neuronal growth and differentiation. Furthermore, type 1 astrocytes express steroid receptors, presenting the possibility that steroid actions on GnRH neurons might occur via astrocytes. Utilizing GT1-7 cells, a GnRH-secreti...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000059434
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND:In 2014, the World Health Organization (WHO) released a classification system introducing neuroendocrine neoplasms (NENs) of the female reproductive tract, excluding the ovaries. This study aimed to evaluate whether retrospective adaption of the gastroenteropancreatic (GEP)-NEN classification is feasible for...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000506743
更新日期:2020-02-25 00:00:00
abstract::Studies were undertaken to characterize the secretion of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) into the hypophysial-portal circulation of the conscious sheep. In addition, we examined the temporal relationship between the secretion of these two hypothalamic peptides and the secretion of t...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125141
更新日期:1989-04-01 00:00:00
abstract::The present study provides evidence of a novel neuronal pathway for the control of GnRH secretion involving bradykinin neurons. Bradykinin neurons were shown by immunohistochemistry to be densely localized in several regions of the brain including the cortex, hippocampus and supraoptic nucleus, as well as two regions ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054316
更新日期:1998-04-01 00:00:00
abstract::Pituitary transcription factor-1 (Pit-1 or GHF-1) is a transcription factor specific to the anterior pituitary and is involved in the expression and regulation of the growth hormone (GH), prolactin (PRL) and thyroid-stimulating hormone (TSH) beta-subunit genes. The expression of these three genes can be modulated by c...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126930
更新日期:1996-01-01 00:00:00
abstract::Brain angiotensin II (AII) receptors play a role in the regulation of luteinizing hormone release. This action is thought to involve luteinizing hormone-releasing hormone containing neurons in the preoptic-anterior hypothalamic (POAH) area of the brain. Previous studies have demonstrated a discrete locus of AII recept...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126225
更新日期:1992-08-01 00:00:00
abstract::Previous work in many laboratories has shown that luteinizing hormone-releasing hormone (LHRH) neurons display a range of shapes from smooth-contoured to extremely irregular or 'thorny'. To determine if these forms reflect or can be modified by the gonadal steroid milieu, the morphology of LHRH neurons was examined in...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125138
更新日期:1989-04-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) is involved in the regulation and maintenance of gametogenesis. It exists in multiple molecular forms with different oligosaccharide structures which in turn are influenced by the hormonal milieu. Previous studies from our laboratory demonstrated that antiandrogen administration to i...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054484
更新日期:1999-10-01 00:00:00
abstract::Unlike rats, but similar to primates, guinea pigs exhibit prolonged function of the corpus luteum and elevated progesterone secretion after ovulation. The gonadotropins, estrogen (E) and progesterone (P) have been examined throughout the guinea pig estrous cycle. However, neither prolactin secretion nor its regulation...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126897
更新日期:1995-06-01 00:00:00
abstract::The effects of angiotensin II (AII) and natriuretic peptide (ANP), alone or in combination, on tuberoinfundibular dopaminergic (TIDA) neuronal activity and on serum PRL levels were examined in this study. The TIDA neuronal activity was determined by measuring 3,4-dihydroxyphenylacetic acid (DOPAC) concentration in the...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127254
更新日期:1997-11-01 00:00:00
abstract::An electrophysiological investigation was conducted on the connection of medial preoptic neurons with the median eminence and the basal and corticomedial amygdala in pentobarbital anesthesized male Sprague-Dawley rats. Of 411 medial preoptic neurons tested with median eminence stimuli, antidromic responses (mean laten...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123237
更新日期:1981-01-01 00:00:00
abstract::The hypothalamic preoptic area (POA) was electrically stimulated through permanently implanted electrodes in 4 unanesthetized sheep. Initiation of POA stimulation was followed witin 10-20 min by large increases in plasma levels of prolactin (PRL). The stimulation-induced discharges of PRL were consistently observed in...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122384
更新日期:1975-01-01 00:00:00
abstract::At present there is no clinical guideline or standardised protocol for the treatment of metastatic or invasive phaeochromocytoma and paraganglioma (collectively known as PPGL) due to the rarity of the disease and the lack of prospective studies or extended national databases. Prognosis is mainly determined by genetic ...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000499497
更新日期:2019-01-01 00:00:00
abstract::Corticotropin-releasing hormone (CRH) plays a key role in adjusting the basal and stress-activated hypothalamic-pituitary-adrenal axis (HPA). CRH is also widely distributed in extrahypothalamic circuits, where it acts as a neuroregulator to integrate the complex neuroendocrine, autonomic, and behavioral adaptive respo...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000328226
更新日期:2011-01-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP), a prolactin (PRL)-releasing factor, has been shown to be synthesized within the anterior pituitary. To test the hypothesis that estrogens increase PRL secretion, at least in part, by stimulating VIP secretion, the concentrations of VIP, peptide histidine isoleucine (PHI) and prepro...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125622
更新日期:1990-11-01 00:00:00
abstract::The acute effects of hypothalamic electrical stimulation on the secretion of FSH and LH have been examined in anestrous and estrous ferrets. Basal levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were higher in the anestrous than in the estrous animals; the anestrous animals showed a more mark...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122674
更新日期:1977-01-01 00:00:00
abstract::To study the eyes' role in maintaining the circadian rhythm in pituitary-adrenal function, 24-h patterns of corticosterone levels were compared in intact and blinded adult female rats. Rats were blinded by optic enucleation at approximately 80 days of age. Nonstress plasma corticosterone levels were determined fluorom...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122495
更新日期:1976-01-01 00:00:00
abstract::Bilateral adrenalectomy (ADX) leads to increased ACTH synthesis and secretion. It is thought that endogenous glucocorticoids exert a feedback mechanism at both pituitary and brain levels. The present study has been performed in order to determine the effect of ADX on the release of hypothalamic neuropeptides with cort...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125713
更新日期:1991-02-01 00:00:00
abstract::Neuroanatomical attempts have been made to determine the synapses between luteinizing hormone-releasing hormone (LHRH)-containing neurons and substance P (SP)-containing neurons in the hypothalamus of female rats. Wheat germ agglutinin was injected into the septo-preoptic area (SPA) and found to be incorporated into c...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125724
更新日期:1991-03-01 00:00:00
abstract::Interleukin-1 (IL-1) and glucocorticoid hormones represent two key mediators involved in the modulation of the neuroimmunoendocrine response to stress. IL-1 is a potent activator of the hypothalamic-pituitary-adrenal (HPA) axis in rodents. In the immune system, glucocorticoids modulate IL-1 production and a number of ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126648
更新日期:1994-02-01 00:00:00
abstract::The inhibitory effects of pituitary allografts on the prolactin (PRL)-secretory system are presumed to be consequences of the unabated release of PRL by the allografts. In the present studies we used pituitary allografts in the Golden Syrian hamster to address the following questions: (a) Do allografts of adult adenoh...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127068
更新日期:1996-05-01 00:00:00
abstract::His-dTrp-Ala-Trp-dPhe,Lys-NH2(GHRP-6) is a synthetic compound that releases GH in a dose-response and specific manner in several species and that may well be related to an endogenous compound of similar structure. The aim of this study was to investigate the in vivo GH responses to GHRP-6 in pentobarbital anesthetized...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126366
更新日期:1993-01-01 00:00:00
abstract::Steroid autoradiography was undertaken to determine the neuroanatomical loci which might be involved in the activation of steroid-sensitive behaviors in the Japanese quail (Coturnix japonica). Male and female quail were either surgically gonadectomized or photically regressed and implanted with androgen or estrogen to...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125091
更新日期:1989-01-01 00:00:00
abstract::Hypertension in male and aging female rodents is associated with glutamate-dependent plasticity in the hypothalamus, but existing models have failed to capture distinct transitional menopausal phases that could have a significant impact on the synaptic plasticity and emergent hypertension. In rodents, accelerated ovar...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000446073
更新日期:2017-01-01 00:00:00
abstract::The effect of pinealectomy or immunization against melatonin (Mel) and N-acetylserotonin (NAS) on plasma prolactin (Prl) levels was studied in rats following pineal stimulation induced by blinding or exposure to short photoperiods (1 h light and 23 h darkness daily). Blinding alone or together with pinealectomy or imm...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122650
更新日期:1977-01-01 00:00:00
abstract::The involvement of histaminergic transmission in the rapid and sustained plasma ACTH and corticosterone (CORT) responses induced in conscious rats by intra-arterial infusions of 25 micrograms.kg-1 Escherichia coli lipopolysaccharide (LPS) was investigated. LPS challenge produced a rapid and transient increase (+ 62%) ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126961
更新日期:1996-03-01 00:00:00
abstract::The effect of estrogen on prolactin (PRL) synthesis at a single-cell level was studied by in situ hybridization and immunohistochemistry. Long-term estrogen treatment increased PRL-containing cells from 10-20% of total cell population to 80-90%, as revealed by immunohistochemistry. PRL mRNA containing cells also incre...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124493
更新日期:1986-01-01 00:00:00
abstract::In order to evaluate the possible participation of the hypothalamic excitatory and inhibitory amino acid neurotransmitter systems in the GnRH release response to GABAergic drugs, hypothalami (preoptic and mediobasal area) of immature (26 days of age) and adult male rats were perifused with GABA-A and -B agonists and a...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127133
更新日期:1996-10-01 00:00:00
abstract::The effect of stress- or lipopolysaccharide (LPS) endotoxin-induced release of ACTH, beta-endorphin (beta-END) and prolactin (PRL) was investigated in two groups of conscious male rats: (1) Rats pretreated with different H3 receptor agonists, which inhibit neuronal histamine (HA) synthesis and release, and (2) rats wi...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054402
更新日期:1999-01-01 00:00:00