Abstract:
:Hematopoietic stem cells purified from mouse bone marrow are quiescent with less than 2% of Lin- Hoechst(low)/Rhodamine(low) (Lin- Ho(low)/Rho(low)) and 10% to 15% of Lin-/Sca+ cells in S phase. These cells enter proliferative cycle and progress through G1 and into S phase in the presence of cytokines and 5% heat-inactivated fetal calf serum (HI-FCS). Cytokine-stimulated Lin- Ho(low)/Rho(low) cells took 36 to 40 hours to complete first division and only 12 hours to complete each of 5 subsequent divisions. These cells require 16 to 18 hours to transit through G0/G1 period and 28 to 30 hours to enter into mid-S phase during the first cycle. Up to 56% of Lin- Rho(low)/Ho(low) cells are high-proliferative potential (7 factor-responsive) colony-forming cells (HPP-CFC). At isolation, HPP-CFC are quiescent, but after 28 to 30 hours of culture, greater than 60% are in S phase. Isoleucine-deprivation of Lin- Ho(low)/Rho(low) cells in S phase of first cycle reversibly blocked them from entering into second cycle. After the release from isoleucine-block, these cells exhibited a G1 period of less than 2 hours and entered into mid-S phase by 12 hours. Thus, the duration of G1 phase of the cells in second cycle is 4 to 5 times shorter than that observed in their first cycle. Similar cell cycle kinetics are observed with Lin-/Sca+ population of bone marrow cells. Stem cell factor (SCF) alone, in the presence of HI-FCS, is as effective as a cocktail of 2 to 7 cytokines in inducing quiescent Lin-/Sca+ cells to enter into proliferative cycle. Aphidicolin treatment reversibly blocked cytokine-stimulated Lin-/Sca+ cells at G1/S boundary, allowing their tight synchrony as they progress through first S phase and enter into second G1. For these cells also, SCF alone is sufficient for their progression through S phase. These studies indicate a very short G1 phase for stem cells induced to proliferate and offer experimental approaches to synchronize murine hematopoietic stem cells.
journal_name
Bloodjournal_title
Bloodauthors
Reddy GP,Tiarks CY,Pang L,Wuu J,Hsieh CC,Quesenberry PJsubject
Has Abstractpub_date
1997-09-15 00:00:00pages
2293-9issue
6eissn
0006-4971issn
1528-0020journal_volume
90pub_type
杂志文章相关文献
BLOOD文献大全abstract::Phospholipase A(2) type IVA (IVAPLA(2)) is a cytosolic enzyme that on activation selectively releases arachidonic acid (AA) from cell membrane phospholipids. Both AA and lysophospholipid, products of the enzymic reaction, can function as signal transducers in cellular interactions. The enzyme is present in most cells,...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-09-2698
更新日期:2004-05-01 00:00:00
abstract::Immune thrombocytopenias are usually associated with normal or increased numbers of megakaryocytes in the marrow. Therefore, the mechanism(s) responsible for the destruction of circulating platelets may not affect megakaryocytes in the same way. One of the possibilities which could account for the differential effect ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Recent data suggest that CD8+ T-cell effector activity is an important component in the control of HIV replication in elite controllers (ECs). One critical element of CD8+ T-cell effector function and differentiation is the T-box transcription factor T-bet. In the present study, we assessed T-bet expression, together ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-12-322727
更新日期:2011-04-07 00:00:00
abstract::The epidemic form of the hemolytic uremic syndrome (HUS), beginning with an acute gastroenteritis, has been associated with a verocytotoxin-producing Escherichia coli infection. The endothelial cell is believed to play an important role in the pathogenesis of HUS. Endothelial cell damage by verocytotoxin-1 (VT-1) in v...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Production of a red blood cell's hemoglobin depends on mitochondrial heme synthesis. However, mature red blood cells are devoid of mitochondria and rely on glycolysis for ATP production. The molecular basis for the selective elimination of mitochondria from mature red blood cells remains controversial. Recent evidence...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-02-137398
更新日期:2008-08-15 00:00:00
abstract::Telomere length has been linked to disease stage and degree of (pan-)cytopenia in patients with bone marrow failure syndromes. The aim of the current study was to analyze the impact of replicative stress on telomere length in residual glycosylphosphatidylinositol-positive (GPI+) versus GPI- hematopoiesis in patients w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-10-3996
更新日期:2005-07-15 00:00:00
abstract::The translocation between chromosomes 8 and 21, t(8;21) (q22;q22), is the most frequent abnormality in acute myeloid leukemia (AML) with French-American-British type M2 (FAB-M2) morphology. The breakpoints in this translocation have been characterized at the molecular level, and the genes involved are AML1 on chromoso...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::The localization of memory T cells to human skin is essential for long-term immune surveillance and the maintenance of barrier integrity. Although the mechanisms controlling memory T-cell migration to peripheral tissues are poorly understood, the current paradigm includes the localized secretion of "imprinting" signal...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-05-433037
更新日期:2012-11-29 00:00:00
abstract::The recruitment of phagocytic leukocytes to sites of vessel wall injury plays an important role in thrombus dissolution by proteases elaborated on their adhesion. However, leukocyte adhesion to the fibrin clot can be detrimental at the early stages of wound healing when hemostatic plug integrity is critical for preven...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-05-022764
更新日期:2007-02-15 00:00:00
abstract::Heme is an essential cofactor for numerous cellular functions, but release of free heme during hemolysis results in oxidative tissue damage, vascular dysfunction, and inflammation. Macrophages play a key protective role in heme clearance; however, the mechanisms that regulate metabolic adaptations that are required fo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020004964
更新日期:2020-09-24 00:00:00
abstract::Previous studies showed that Fanconi anemia (FA) murine stem cells have defective reconstitution after bone marrow (BM) transplantation. The mechanism underlying this defect is not known. Here, we report defective homing of FA patient BM progenitors transplanted into mouse models. Using cells from patients carrying mu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-03-147090
更新日期:2008-09-01 00:00:00
abstract::Human B-cell lines were derived by limiting dilutions of Epstein-Barr virus (EBV) transformed peripheral B cells from a patient with an autoantibody against glycoprotein (GP)Ia/IIa, and manifesting defective collagen-induced platelet aggregation and a bleeding problem. Antibody-producing clones were selected for their...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-09-15 00:00:00
abstract::Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganization and spreading. These responses depend on tyrosine phosphorylation of multiple proteins by Src family members and Syk. Among Src substrates in platelets is adhesion- and degranulation-promoting adapter protein (ADAP), a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-08-238238
更新日期:2010-03-18 00:00:00
abstract::Cellular oxygen partial pressure is sensed by a family of prolyl-4-hydroxylase domain (PHD) enzymes that modify hypoxia-inducible factor (HIF)alpha subunits. Upon hydroxylation under normoxic conditions, HIFalpha is bound by the von Hippel-Lindau tumor suppressor protein and targeted for proteasomal destruction. Since...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-10-3980
更新日期:2005-06-15 00:00:00
abstract::Staining with CD14 and CD16 monoclonal antibodies will identify two monocyte subpopulations in human blood: a major population of regular monocytes, which strongly expresses the CD14 antigen (CD14++), and a minor population with weak expression of CD14 and expression of the CD16 antigen (CD14+/CD16+ cells). As shown h...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-11-15 00:00:00
abstract::A new human leukemia cell line, KT-1, was established from a patient in the blastic crisis phase of chronic myelogenous leukemia (CML). This cell line had a positive reaction for intracytoplasmic myeloperoxidase and two Philadelphia chromosomes (Ph1) [t(9;22)(q34;q11)] and lacked normal copies of chromosomes 9 and 22....
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-01-15 00:00:00
abstract::Graft-versus-host disease (GVHD) is driven by extensive activation and proliferation of alloreactive donor T cells causing significant morbidity and mortality following allogeneic hematopoietic cell transplantation (HCT). Invariant natural killer T (iNKT) cells are a potent immunoregulatory T-cell subset in both human...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-11-612762
更新日期:2015-05-28 00:00:00
abstract::Regulatory molecules produced by stromal cells are often membrane bound until cleaved by matrix metalloproteinases (MMPs); cleavage can either activate or inactivate regulatory functions. We report here that marrow stromal cells induce the expression of MMP-9 in monocytes. Induction was contact independent and could b...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-05-020289
更新日期:2007-01-01 00:00:00
abstract::To clarify the nature of null cell acute lymphoblastic leukemia (ALL) we tested null lymphoblasts and other leukemic cells for human T and B lymphocyte antigens detected by reciprocally absorbed rabbit antisera prepared against autologous T and B lymphoblast cell lines HSB-2 and SB. Blasts from 4 of 4 patients with T ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::RUNX1 is crucial for the regulation of megakaryocyte specification, maturation, and thrombopoiesis. Runx1 possesses 2 promoters: the distal P1 and proximal P2 promoters. The major protein isoforms generated by P1 and P2 are RUNX1C and RUNX1B, respectively, which differ solely in their N-terminal amino acid sequences. ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-06-723635
更新日期:2017-07-20 00:00:00
abstract::Several signaling cascades are engaged by expression of the p210 bcr-abl tyrosine kinase, and evidence suggests that these signals drive leukemogenesis. In this report, signaling pathways were examined and compared between cells derived from leukemic patients and cells expressing a bcr-abl construct (MBA). The effects...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.9.2846
更新日期:2001-05-01 00:00:00
abstract::The transforming growth factor-beta (TGF-beta) family of cytokines regulates vascular development and inflammatory responses. We have recently shown that exposure of human umbilical vein endothelial cells (HUVECs) to hypoxia (1% O(2)) increases gene expression and bioactivation of TGF-beta2 and induces its downstream ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-02-0629
更新日期:2003-03-15 00:00:00
abstract::Human plasma fibrinogen is produced by liver parenchymal cells. Such molecules contain two classes of gamma-chains (gamma A, gamma'), which differ with respect to their COOH-terminal sequences. When fibrin is crosslinked in the presence of factor XIIIa and Ca2+, three types of gamma-dimer are formed (gamma A-gamma A; ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Noncovalent association between the von Willebrand factor (VWF) propeptide (VWFpp) and mature VWF aids N-terminal multimerization and protein compartmentalization in storage granules. This association is currently thought to dissipate after secretion into blood. In the present study, we examined this proposition by qu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-10-387548
更新日期:2012-05-17 00:00:00
abstract::The non-Hodgkin's lymphoma (NHL) subset commonly referred to as large cell lymphoma (LCL) has historically been characterized by it's marked cytological, immunological, and clinical heterogeneity. One potential defining feature of these lymphomas, the t(2;5)(p23;q35), occurs in 25% to 30% of anaplastic LCLs and is als...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::T-cell depletion (TCD) of the donor marrow graft has been shown to reduce the severity of graft-versus-host disease (GVHD) in patients with chronic-phase (CP) chronic myelogenous leukemia (CML) undergoing HLA-identical sibling allogeneic marrow transplantation. However, there has been a corresponding reduction in the ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-07-15 00:00:00
abstract::In this issue of Blood, Rieger et al show that malaria parasite infiltration in the human placenta requires a specific geometry and affinity of host receptors to facilitate strong adhesion. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2014-11-610634
更新日期:2015-01-08 00:00:00
abstract::The Kindlin family of intracellular proteins has recently emerged as key regulators of cellular functions and cell-matrix interactions. The 3 members of this family, Kindlin-1, -2, and -3, perform an essential role in activation of integrin adhesion receptors, and expression of at least 1 Kindlin paralog is required t...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2009-10-239269
更新日期:2010-05-20 00:00:00
abstract::Nineteen noninfected adults receiving initial induction chemotherapy for acute nonlymphocytic leukemia (ANLL) were randomized to receive either prophylactic granulocyte transfusion or platelet transfusion alone on an alternate-day schedule. An average of 11 granulocyte transfusions (range 3--19) were administered/pati...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1979-10-01 00:00:00
abstract::Integrin-β7 (ITGB7) mRNA is detected in multiple myeloma (MM) cells and its presence is correlated with MAF gene activation. Although the involvement of several integrin family members in MM-stoma cell interaction is well documented, the specific biologic functions regulated by integrin-β7 in MM are largely unknown. C...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-06-292243
更新日期:2011-06-09 00:00:00