Abstract:
:Human plasma fibrinogen is produced by liver parenchymal cells. Such molecules contain two classes of gamma-chains (gamma A, gamma'), which differ with respect to their COOH-terminal sequences. When fibrin is crosslinked in the presence of factor XIIIa and Ca2+, three types of gamma-dimer are formed (gamma A-gamma A; gamma A-gamma'; gamma'-gamma'). A separate fibrinogen pool is located in platelet alpha-granules. We analyzed this fibrinogen to determine whether gamma'-chains were present to the same extent (7%) that they are found in plasma fibrinogen. Electrophoretic analysis (Laemmli system) of reduced samples of the clot that formed subsequent to release of fibrinogen from thrombin-stimulated washed platelets, revealed a single crosslinked gamma-dimer band in the gamma A-gamma A position. Material collected into EDTA-containing buffer and subsequently crosslinked in the presence of added factor XIII and Ca2+ also revealed a gamma A-gamma A dimer band. This finding was further investigated by Western blotting of reduced gel specimens that had been reacted with an anti-gamma-chain antibody followed by 125I-labeled protein A. A single type of gamma-chain, gamma A, was present in the fibrinogen from a Triton X-100 or 10 M urea platelet lysate, or in noncrosslinked fibrin obtained from thrombin-treated platelets. Crosslinked reduced fibrin from thrombin-treated platelets or that prepared from the Triton lysate revealed a single type of gamma-dimer, gamma A-gamma A. We conclude that there are no gamma'-chains (less than 1%) in platelet fibrinogen. This structural difference from hepatic fibrinogen probably results from differences in the processing and/or regulation of the fibrinogen gamma-chain gene in megakaryocytes.
journal_name
Bloodjournal_title
Bloodauthors
Mosesson MW,Homandberg GA,Amrani DLsubject
Has Abstractpub_date
1984-05-01 00:00:00pages
990-5issue
5eissn
0006-4971issn
1528-0020journal_volume
63pub_type
杂志文章相关文献
BLOOD文献大全abstract::Surface marker and gene rearrangement data have supported various hypotheses about the origin of the malignant cell in Hodgkin's disease. Cytogenetic data about this disorder, however, are very scanty. To determine if any chromosomal abnormalities that could add further information to this controversial point are pres...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) consists of at least 2 phenotypic subtypes; that is, the germinal center B-cell-like (GCB-DLBCL) and the activated B-cell-like (ABC-DLBCL) groups. It has been shown that GCB-DLBCL responds favorably to chemotherapy and expresses high levels of BCL6, a transcription repressor known...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-04-087734
更新日期:2008-02-01 00:00:00
abstract::To identify new cytogenetic abnormalities associated with leukemogenesis or disease outcome, T-cell acute lymphoblastic leukemia (T-ALL) patient samples were analyzed by means of the array-comparative genome hybridization technique (array-CGH). Here, we report the identification of a new recurrent and cryptic deletion...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-04-019927
更新日期:2006-11-15 00:00:00
abstract::Atypical hemolytic uremic syndrome (aHUS) is a severe renal disease that is associated with defective complement regulation caused by multiple factors. We previously described the deficiency of factor H-related proteins CFHR1 and CFHR3 as predisposing factor for aHUS. Here we identify in an extended cohort of 147 aHUS...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-09-109876
更新日期:2008-02-01 00:00:00
abstract::Pretreatment cytogenetics is a known predictor of outcome in hematologic malignancies. However, its usefulness in adult acute lymphoblastic leukemia (ALL) is generally limited to the presence of the Philadelphia (Ph) chromosome because of the low incidence of other recurrent abnormalities. We present centrally reviewe...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1182/blood-2006-10-051912
更新日期:2007-04-15 00:00:00
abstract::Patients with antineutrophil cytoplasmic antibodies (ANCAs) frequently develop severe vasculitis and glomerulonephritis. Although ANCAs, particularly antimyeloperoxidase (anti-MPO), have been shown to promote leukocyte adhesion in postcapillary venules, their ability to promote adhesion in the glomerular vasculature i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-12-192617
更新日期:2009-06-18 00:00:00
abstract::Wiskott-Aldrich syndrome (WAS) pediatric patients exhibit a deficiency in humoral immune memory. However, the mechanism by which Wiskott-Aldrich syndrome protein (WASP) regulates the differentiation and activation of memory B cells remains elusive. Here we examine the early activation events of memory B cells from the...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-03-703579
更新日期:2016-09-29 00:00:00
abstract::Intrachromosomal amplification of chromosome 21 (iAMP21) defines a distinct subgroup of childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL) that has a dismal outcome when treated with standard therapy. For improved diagnosis and risk stratification, the initiating genetic events need to be elucidated. To...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-01-329961
更新日期:2011-06-23 00:00:00
abstract::We administered gemtuzumab ozogamycin ("mylotarg"; 9 mg/m(2) day 1 or 5) and all-trans retinoic acid (ATRA) to 19 patients with untreated acute promyelocytic leukemia (APL). There were 3 patients who also received idarubicin because of a white blood cell (WBC) count of more than 30 000/microL. In complete remission (C...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2001-12-0174
更新日期:2002-06-01 00:00:00
abstract::The transcription factor signal transducer and activator of transcription 5 (STAT5) fulfills essential roles in self-renewal in mouse and human hematopoietic stem cells (HSCs), and its persistent activation contributes to leukemic transformation, although little molecular insight into the underlying mechanisms has bee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-303669
更新日期:2011-03-24 00:00:00
abstract::The microsomal fraction of M1 cells (an established cell line of myeloid leukemia) was capable of catalyzing acylation of sn-glycerol 3-phosphate by long-chain fatty acyl-CoA thioesters. The principal lipid product formed was identified as phosphatidic acid. Palmityl-CoA, stearyl-CoA, and oleyl-CoA were more effective...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::In this issue of Blood, Bartel and colleagues report the independent predictive value of the PET/CT and of the FDG suppression before transplantation in newly diagnosed myeloma patients who were treated using the TT3 regimen. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2009-06-224196
更新日期:2009-09-03 00:00:00
abstract::The clinical, morphological, immunologic, and cytogenetic features of seven cases of chronic granulated T cell lymphocytosis with neutropenia were studied. The disorder was characterized by moderate blood and bone marrow lymphocytosis, neutropenia, polyclonal hypergammaglobulinemia, splenomegaly, absence of lymphadeno...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::Low molecular weight heparin (LMWH) is being tested as an experimental drug for improving pregnancy outcome in women with inherited thrombophilia and placenta-mediated pregnancy complications, such as recurrent pregnancy loss. The role of thrombotic processes in these disorders remains unproven, and the issue of antit...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-08-448209
更新日期:2013-03-14 00:00:00
abstract::We have shown previously that EM011, a synthetic compound, binds tubulin with a higher affinity than the founding compound, noscapine, without changing total microtubule polymer mass. Now we show that EM011 is potently effective against vinblastine-resistant human lymphoblastoid line CEM/VLB100 and its parental vinbla...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-08-3516
更新日期:2006-03-15 00:00:00
abstract::Although mycosis fungoides (MF) is typically an indolent disease, patients with advanced-stage disease (stages IIB-IVB), including Sézary syndrome (SS), often have a poor outcome. A 31-year, retrospective analysis of our cutaneous lymphoma database, of 297 patients with MF and SS, was undertaken to study long-term out...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-05-154609
更新日期:2008-10-15 00:00:00
abstract::In an attempt to decrease the relapse rate after bone marrow transplantation (BMT) for advanced acute leukemia, we initiated studies using 131I-labeled anti-CD45 antibody (BC8) to deliver radiation specifically to hematopoietic tissues, followed by a standard transplant preparative regimen. Biodistribution studies wer...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-02-15 00:00:00
abstract::Human promyelocytic leukemia (HL-60) cells were induced to differentiate into macrophage-like cells by treatment with 10(-7) mol/L 1,25-dihydroxyvitamin D3 (VD3). A monoclonal antibody (MoAb, 60B8), reactive with the particulate of the differentiated cells but not of the untreated cells, was isolated. The antigen reco...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprec...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-08-800359
更新日期:2018-03-01 00:00:00
abstract::Marrow specimens obtained from 23 patients with acute myelocytic leukemia were exposed to cytosine arabinoside and/or daunorubicin in vitro, and the effects of these agents on colony formation in vitro was determined. Thymidine suicide indices were determined as well, which permitted a distinction to be made between k...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::The t(1;19)(q23;p13) chromosomal translocation is observed cytogenetically in 25% of children with pre-B-cell acute lymphoblastic leukemia (ALL) and is associated with an adverse treatment outcome. The t(1;19) juxtaposes the E2A gene from chromosome 19 with the PBX1 gene on chromosome 1, leading to the production of f...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-02-15 00:00:00
abstract::In previous studies, the prevalence of HLA-linked hemochromatosis, thought to be the most common genetic illness in whites, has been estimated by identifying homozygotes in the population. Because not all homozygotes express the disease phenotypically, the accuracy of these estimates is uncertain. We analyzed the dist...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::A pivotal role has been assigned to Myb in the control of myeloid cell growth. Although Myb is a target of retinoic acid, little is known about the mechanisms by which it may contribute to induced growth arrest in leukemia cells. Indeed, few Myb target genes are known to be linked to proliferation. Myeloblastin is inv...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.8.2449
更新日期:2001-04-15 00:00:00
abstract::Identification of receptor activator of nuclear factor-kappaB (RANK) and RANK-ligand (RANKL) has provided new insights into the osteoclast differentiation pathway. Osteoclast precursor cells were isolated using monoclonal antibodies against c-Fms and RANK, and the effect of adherence on the in vitro differentiation an...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-12-15 00:00:00
abstract::An unmet need in cell engineering is the availability of a single transgene encoded, functionally inert, human polypeptide that can serve multiple purposes, including ex vivo cell selection, in vivo cell tracking, and as a target for in vivo cell ablation. Here we describe a truncated human EGFR polypeptide (huEGFRt) ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-337360
更新日期:2011-08-04 00:00:00
abstract::A common genetic risk factor for venous thrombosis among Caucasoid subpopulations is a polymorphism, nt G1691A, in blood coagulation factor V that replaces Arg506 with Gln and imparts resistance of factor Va to the anticoagulant, activated protein C. Haplotype analyses using six dimorphic sites in the factor V gene fo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-01-15 00:00:00
abstract::T cells are known to interact cooperatively with monocytes to produce Colony-Stimulating Factors (CSF), although T cell-mediated signals leading to CSF secretion by monocytes are not completely understood. We have made use of Northern blot hybridization and specific bioassays to study the effects of the T cell product...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::Proteins exported from Plasmodium falciparum parasites into red blood cells (RBCs) interact with the membrane skeleton and contribute to the pathogenesis of malaria. Specifically, exported proteins increase RBC membrane rigidity, decrease deformability, and increase adhesiveness, culminating in intravascular sequestra...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-05-157735
更新日期:2009-01-22 00:00:00
abstract::The class IA subgroup of phosphoinositide 3-kinase (PI3K) is activated downstream of antigen receptors, costimulatory molecules, and cytokine receptors on lymphocytes. Targeted deletion of individual genes for class IA regulatory subunits severely impairs the development and function of B cells but not T cells. Here w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-07-038620
更新日期:2007-04-01 00:00:00
abstract::Activation and subsequent differentiation of naive CD8+ T cells lead to the development of memory subsets with distinct homing and effector capacities. On nonlymphoid homing subsets, expression of "inflammatory" chemokine receptors (such as CXCR3, CCR5, CX3CR1, and CXCR1) is believed to promote migration into sites of...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-04-1366
更新日期:2005-12-01 00:00:00