The t(1;19)(q23;p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias.


:The t(1;19)(q23;p13) chromosomal translocation is observed cytogenetically in 25% of children with pre-B-cell acute lymphoblastic leukemia (ALL) and is associated with an adverse treatment outcome. The t(1;19) juxtaposes the E2A gene from chromosome 19 with the PBX1 gene on chromosome 1, leading to the production of fusion transcripts and resultant chimeric proteins that contain the transcriptional-activating motif of E2A and the DNA-binding homeodomain of PBX1. To investigate the molecular nature of E2A/PBX1 fusion in patients with t(1;19) ALL we used an RNA-based polymerase chain reaction (PCR) procedure to amplify a portion of the chimeric transcript. We detected E2A/PBX1 fusion transcripts in cells from 97% (37 of 38) of cases in which the t(1;19) had been observed cytogenetically. Molecular evidence of E2A/PBX1 fusion transcripts was also observed in a patient in whom a t(1;19) was not detected cytogenetically and in one patient with subclinical levels of minimal residual disease before overt clinical relapse. In all PCR-positive cases the junction of E2A and PBX1 coding sequences occurred at precisely the same location as demonstrated by hybridization of PCR products with a fusion site-specific detection oligonucleotide. These findings demonstrate the consistent fusion of E2A and PBX1 coding sequences resulting from t(1;19) and suggest that site-specific fusion of E2A and PBX1 is an important pathogenic event in t(1;19) ALL.






Hunger SP,Galili N,Carroll AJ,Crist WM,Link MP,Cleary ML


Has Abstract


1991-02-15 00:00:00














  • Interferon-gamma upregulates interleukin-8 gene expression in human monocytic cells by a posttranscriptional mechanism.

    abstract::Interleukin-8 (IL-8) is a neutrophil chemotactic and activating cytokine that is produced in response to several stimuli. Because monocytic cells are important producers of IL-8, we investigated whether interferon-gamma (IFN-gamma), a potent inducer of activation and differentiation of mononuclear phagocytes, affected...


    pub_type: 杂志文章


    authors: Bosco MC,Gusella GL,Espinoza-Delgado I,Longo DL,Varesio L

    更新日期:1994-01-15 00:00:00

  • Acquired FANCA dysfunction and cytogenetic instability in adult acute myelogenous leukemia.

    abstract::Myelodysplastic and leukemic stem cell clones that evolve in children and adults with Fanconi anemia universally bear complex cytogenetic abnormalities. The abnormalities are generally recurring deletions or chromosomal loss and involve precisely the same chromosomes with the same frequency as has been described in ma...


    pub_type: 杂志文章


    authors: Lensch MW,Tischkowitz M,Christianson TA,Reifsteck CA,Speckhart SA,Jakobs PM,O'Dwyer ME,Olson SB,Le Beau MM,Hodgson SV,Mathew CG,Larson RA,Bagby GC Jr

    更新日期:2003-07-01 00:00:00

  • New insights into the mechanisms of hematopoietic cell transformation by activated receptor tyrosine kinases.

    abstract::A large number of alterations in genes encoding receptor tyrosine kinase (RTK), namely FLT3, c-KIT, platelet-derived growth factor (PDGF) receptors, fibroblast growth factor (FGF) receptors, and the anaplastic large cell lymphoma kinase (ALK), have been found in hematopoietic malignancies. They have drawn much attenti...


    pub_type: 杂志文章,评审


    authors: Toffalini F,Demoulin JB

    更新日期:2010-10-07 00:00:00

  • Localization of ADAMTS13 to the stellate cells of human liver.

    abstract::Although the chromosomal localization (9q34) of the gene encoding the human form of ADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13) and its exclusive expression in the liver have been established, the cells that produce this enzyme are yet to be determined. We investigated the ...


    pub_type: 杂志文章


    authors: Uemura M,Tatsumi K,Matsumoto M,Fujimoto M,Matsuyama T,Ishikawa M,Iwamoto TA,Mori T,Wanaka A,Fukui H,Fujimura Y

    更新日期:2005-08-01 00:00:00

  • p53 at the crossroads of MPN treatment.

    abstract::In this issue of Blood, Lu et al describe the cooperation between an orally bioavailable mouse double minute 2 (MDM2) antagonist (RG7112) and the pegylated interferon α (Peg-IFNα 2a) to target JAK2V617F hematopoietic progenitors and stem cells. Their work provides a rationale for the treatment of patients suffering fr...


    pub_type: 评论,杂志文章


    authors: Plo I

    更新日期:2014-07-31 00:00:00

  • Lack of Fc-epsilon receptors on murine eosinophils: implications for the functional significance of elevated IgE and eosinophils in parasitic infections.

    abstract::Chronic infection with Schistosoma mansoni induces in humans and mice a Th2-dominant immune response in which eosinophils and IgE are conspicuously elevated. Human eosinophils express IgE receptors that participate in an IgE-dependent eosinophil-mediated ADCC reaction against Schistosomula larvae in vitro. To investig...


    pub_type: 杂志文章


    authors: de Andres B,Rakasz E,Hagen M,McCormik ML,Mueller AL,Elliot D,Metwali A,Sandor M,Britigan BE,Weinstock JV,Lynch RG

    更新日期:1997-05-15 00:00:00

  • Donor CMV serologic status and outcome of CMV-seropositive recipients after unrelated donor stem cell transplantation: an EBMT megafile analysis.

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    pub_type: 杂志文章


    authors: Ljungman P,Brand R,Einsele H,Frassoni F,Niederwieser D,Cordonnier C

    更新日期:2003-12-15 00:00:00

  • Mice expressing a neutrophil elastase mutation derived from patients with severe congenital neutropenia have normal granulopoiesis.

    abstract::Severe congenital neutropenia (SCN) is a syndrome characterized by an isolated block in granulocytic differentiation and an increased risk of developing acute myeloid leukemia (AML). Recent studies have demonstrated that the majority of patients with SCN and cyclic neutropenia, a related disorder characterized by peri...


    pub_type: 杂志文章


    authors: Grenda DS,Johnson SE,Mayer JR,McLemore ML,Benson KF,Horwitz M,Link DC

    更新日期:2002-11-01 00:00:00

  • Factor VIII-induced superaggregation of human platelets.

    abstract::High concentrations of bovine factor VIII cause clumping of platelets into a few very large aggregates. This response is termed superaggregation. It is distinct from factor-VIII-induced agglutination but is also independent of both extracellular calcium ions and platelet energy metabolism. Neither agglutinating lectin...


    pub_type: 杂志文章


    authors: Kirby EP,Mills DC,Holmsen H,Russo M

    更新日期:1982-12-01 00:00:00

  • Identification of the dombrock blood group glycoprotein as a polymorphic member of the ADP-ribosyltransferase gene family.

    abstract::Identification of the 25 known human blood group molecules is of fundamental importance for the fields of erythroid cell biology and transfusion medicine. Here we provide the first molecular description of the "Dombrock" blood group system. A candidate gene was identified by in silico analyses of approximately 5000 ex...


    pub_type: 杂志文章


    authors: Gubin AN,Njoroge JM,Wojda U,Pack SD,Rios M,Reid ME,Miller JL

    更新日期:2000-10-01 00:00:00

  • Oligodeoxynucleotide-mediated inhibition of c-myb gene expression in autografted bone marrow: a pilot study.

    abstract::Antisense oligodeoxynucleotide (ODN) drugs might be more effective if their delivery was optimized and they were targeted to short-lived proteins encoded by messenger RNA (mRNA) species with equally short half-lives. To test this hypothesis, an ODN targeted to the c-myb proto-oncogene was developed and used to purge m...


    pub_type: 临床试验,杂志文章


    authors: Luger SM,O'Brien SG,Ratajczak J,Ratajczak MZ,Mick R,Stadtmauer EA,Nowell PC,Goldman JM,Gewirtz AM

    更新日期:2002-02-15 00:00:00

  • Platelet-delivered ADAMTS13 inhibits arterial thrombosis and prevents thrombotic thrombocytopenic purpura in murine models.

    abstract::ADAMTS13 metalloprotease cleaves von Willebrand factor (VWF), thereby inhibiting platelet aggregation and arterial thrombosis. An inability to cleave ultralarge VWF resulting from hereditary or acquired deficiency of plasma ADAMTS13 activity leads to a potentially fatal syndrome, thrombotic thrombocytopenic purpura (T...


    pub_type: 杂志文章


    authors: Pickens B,Mao Y,Li D,Siegel DL,Poncz M,Cines DB,Zheng XL

    更新日期:2015-05-21 00:00:00

  • Erythropoietin stimulates a rise in intracellular-free calcium concentration in single BFU-E derived erythroblasts at specific stages of differentiation.

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    pub_type: 杂志文章


    authors: Miller BA,Cheung JY,Tillotson DL,Hope SM,Scaduto RC Jr

    更新日期:1989-04-01 00:00:00

  • Integrin β7-mediated regulation of multiple myeloma cell adhesion, migration, and invasion.

    abstract::Integrin-β7 (ITGB7) mRNA is detected in multiple myeloma (MM) cells and its presence is correlated with MAF gene activation. Although the involvement of several integrin family members in MM-stoma cell interaction is well documented, the specific biologic functions regulated by integrin-β7 in MM are largely unknown. C...


    pub_type: 杂志文章


    authors: Neri P,Ren L,Azab AK,Brentnall M,Gratton K,Klimowicz AC,Lin C,Duggan P,Tassone P,Mansoor A,Stewart DA,Boise LH,Ghobrial IM,Bahlis NJ

    更新日期:2011-06-09 00:00:00

  • Erratum: Airoldi I, Bertaina A, Prigione I, et al. γδ T-cell reconstitution after HLA-haploidentical hematopoietic transplantation depleted of TCR-αβ+/CD19+ lymphocytes. Blood. 2015;125(15):2349-2358.

    abstract::[This corrects the article DOI: 10.1182/blood-2014-09-599423.]. ...


    pub_type: 杂志文章,已发布勘误



    更新日期:2016-03-24 00:00:00

  • HIT-antibodies promote their own antigen.

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    pub_type: 评论,杂志文章


    authors: Greinacher A,Krauel K,Jensch I

    更新日期:2012-08-02 00:00:00

  • Near-haploid and low-hypodiploid acute lymphoblastic leukemia: two distinct subtypes with consistently poor prognosis.

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    pub_type: 杂志文章,评审


    authors: Safavi S,Paulsson K

    更新日期:2017-01-26 00:00:00

  • Acute cholesterol depletion impairs functional expression of tissue factor in fibroblasts: modulation of tissue factor activity by membrane cholesterol.

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    pub_type: 杂志文章


    authors: Mandal SK,Iakhiaev A,Pendurthi UR,Rao LV

    更新日期:2005-01-01 00:00:00

  • Transfusion-associated AIDS: donor-recipient human immunodeficiency virus exhibits genetic heterogeneity.

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    pub_type: 杂志文章


    authors: Srinivasan A,York D,Ranganathan P,Ferguson R,Butler D Jr,Feorino P,Kalyanaraman V,Jaffe H,Curran J,Anand R

    更新日期:1987-06-01 00:00:00

  • Expression of high Km 5'-nucleotidase in leukemic blasts is an independent prognostic factor in adults with acute myeloid leukemia.

    abstract::Cytarabine (ara-C) requires activation into its triphosphorylated form, ara-CTP, to exert cytotoxic activity. Cytoplasmic 5'-nucleotidase (5NT) dephosphorylates ara-CMP, a key intermediate, preventing accumulation of ara-CTP and may reduce cellular sensitivity to the cytotoxic activity of ara-C. To determine whether t...


    pub_type: 杂志文章


    authors: Galmarini CM,Graham K,Thomas X,Calvo F,Rousselot P,El Jafaari A,Cros E,Mackey JR,Dumontet C

    更新日期:2001-09-15 00:00:00

  • A linear amino acid sequence involved in the interaction of t-PA with its endothelial cell receptor.

    abstract::Endothelial cell receptors for tissue plasminogen activator (t-PA) have been demonstrated recently, and we have sought to identify a region of the t-PA molecule involved in its interaction with these receptors on human umbilical vein endothelial cells. Of three monoclonal antibodies against various regions of t-PA, on...


    pub_type: 杂志文章


    authors: Beebe DP,Miles LA,Plow EF

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  • Disparate lymphoid chemokine expression in mice and men: no evidence of CCL21 synthesis by human high endothelial venules.

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    pub_type: 杂志文章


    authors: Carlsen HS,Haraldsen G,Brandtzaeg P,Baekkevold ES

    更新日期:2005-07-15 00:00:00

  • Prevalence and predictors of chronic health conditions after hematopoietic cell transplantation: a report from the Bone Marrow Transplant Survivor Study.

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    pub_type: 杂志文章


    authors: Sun CL,Francisco L,Kawashima T,Leisenring W,Robison LL,Baker KS,Weisdorf DJ,Forman SJ,Bhatia S

    更新日期:2010-10-28 00:00:00

  • All-trans retinoic acid in acute promyelocytic leukemias. II. In vitro studies: structure-function relationship.

    abstract::All-trans retinoic acid induces leukemic cells from patients with acute promyelocytic leukemia (M3) to differentiate in vitro to mature granulocytes which express the CD15 antigen and are capable of respiratory burst function. Of 35 M3 samples, only one failed to respond. In eight cases, we compared the efficacy of tw...


    pub_type: 杂志文章


    authors: Chomienne C,Ballerini P,Balitrand N,Daniel MT,Fenaux P,Castaigne S,Degos L

    更新日期:1990-11-01 00:00:00

  • Ikaros is required for plasmacytoid dendritic cell differentiation.

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    pub_type: 杂志文章


    authors: Allman D,Dalod M,Asselin-Paturel C,Delale T,Robbins SH,Trinchieri G,Biron CA,Kastner P,Chan S

    更新日期:2006-12-15 00:00:00

  • Toll-like receptors and their ligands control mesenchymal stem cell functions.

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    pub_type: 杂志文章


    authors: Pevsner-Fischer M,Morad V,Cohen-Sfady M,Rousso-Noori L,Zanin-Zhorov A,Cohen S,Cohen IR,Zipori D

    更新日期:2007-02-15 00:00:00

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    pub_type: 杂志文章


    authors: Dobrina A,Pausa M,Fischetti F,Bulla R,Vecile E,Ferrero E,Mantovani A,Tedesco F

    更新日期:2002-01-01 00:00:00

  • Substitution of the gamma-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking.

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    pub_type: 杂志文章


    authors: Okumura N,Gorkun OV,Terasawa F,Lord ST

    更新日期:2004-06-01 00:00:00

  • Immunologic characterization of a granulocytic leukemia of inbred strain 13 guinea pigs: presence of Ia-positive myeloblasts.

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    pub_type: 杂志文章


    authors: Clement LT,Hu C,Shevach EM,Green I

    更新日期:1980-02-01 00:00:00

  • Trafficking of the major virulence factor to the surface of transfected P. falciparum-infected erythrocytes.

    abstract::After invading human red blood cells (RBCs) the malaria parasite Plasmodium falciparum remodels the host cell by trafficking proteins to the RBC compartment. The virulence protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) is responsible for cytoadherence of infected cells to host endothelial receptors. Thi...


    pub_type: 杂志文章


    authors: Knuepfer E,Rug M,Klonis N,Tilley L,Cowman AF

    更新日期:2005-05-15 00:00:00