Abstract:
:The phagocytic function of macrophages plays a pivotal role in eliminating apoptotic cells and invading pathogens. Evidence implicating plasminogen (Plg), the zymogen of plasmin, in phagocytosis is extremely limited with the most recent in vitro study showing that plasmin acts on prey cells rather than on macrophages. Here, we use apoptotic thymocytes and immunoglobulin opsonized bodies to show that Plg exerts a profound effect on macrophage-mediated phagocytosis in vitro and in vivo. Plg enhanced the uptake of these prey by J774A.1 macrophage-like cells by 3.5- to fivefold Plg receptors and plasmin proteolytic activity were required for phagocytosis of both preys. Compared with Plg(+/+) mice, Plg(-/-) mice exhibited a 60% delay in clearance of apoptotic thymocytes by spleen and an 85% reduction in uptake by peritoneal macrophages. Phagocytosis of antibody-mediated erythrocyte clearance by liver Kupffer cells was reduced by 90% in Plg(-/-) mice compared with Plg(+/+) mice. A gene array of splenic and hepatic tissues from Plg(-/-) and Plg(+/+) mice showed downregulation of numerous genes in Plg(-/-) mice involved in phagocytosis and regulation of phagocytic gene expression was confirmed in macrophage-like cells. Thus, Plg may play an important role in innate immunity by changing expression of genes that contribute to phagocytosis.
journal_name
Bloodjournal_title
Bloodauthors
Das R,Ganapathy S,Settle M,Plow EFdoi
10.1182/blood-2014-01-549659subject
Has Abstractpub_date
2014-07-31 00:00:00pages
679-88issue
5eissn
0006-4971issn
1528-0020pii
blood-2014-01-549659journal_volume
124pub_type
杂志文章相关文献
BLOOD文献大全abstract::Sickle cell disease is a common hemolytic disorder with a broad range of complications, including vaso-occlusive episodes, acute chest syndrome (ACS), pain, and stroke. Heme oxygenase-1 (gene HMOX1; protein HO-1) is the inducible, rate-limiting enzyme in the catabolism of heme and might attenuate the severity of outco...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-06-361642
更新日期:2012-11-01 00:00:00
abstract::The FAS receptor-FAS ligand system is a key apoptotic pathway for cells of the immune system. Ligation of the FAS-receptor (CD95) induces apoptosis by activation of pro-caspase-8 followed by downstream events, including an increase in reactive oxygen species (ROS) and the release of proapoptotic factors from the mitoc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-11-3559
更新日期:2003-07-01 00:00:00
abstract::Regulated migration of hematopoietic stem cells is fundamental for hematopoiesis. The molecular mechanisms underlying stem cell trafficking are poorly defined. Based on a short hairpin RNA library and stromal cell-derived factor-1 (SDF-1) migration screening assay, we identified the histone 3 lysine 27 demethylase UTX...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-08-452003
更新日期:2013-03-28 00:00:00
abstract::AC133 is one of a new panel of murine hybridoma lines producing monoclonal IgG antibodies (mAbs) to a novel stem cell glycoprotein antigen with a molecular weight of 120 kD. AC133 antigen is selectively expressed on CD34(bright) hematopoietic stem and progenitor cells (progenitors) derived from human fetal liver and b...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-12-15 00:00:00
abstract::Previous studies on patients with storage pool deficiency (SPD) who are specifically deficient in platelet dense granules (delta-SPD) have suggested a role for dense granule substances, in all likelihood adenosine diphosphate (ADP), in mediating thrombus formation on subendothelium at high shear rates. The role of den...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-05-15 00:00:00
abstract::After the discovery of NPM1-mutated acute myeloid leukemia (AML) in 2005 and its subsequent inclusion as a provisional entity in the 2008 World Health Organization classification of myeloid neoplasms, several controversial issues remained to be clarified. It was unclear whether the NPM1 mutation was a primary genetic ...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-08-299990
更新日期:2011-01-27 00:00:00
abstract::Familial hemophagocytic lymphohistiocytosis (FHL) is a frequently missed and almost uniformly fatal childhood disorder. It is characterized by fever, hepatosplenomegaly, cytopenia, coagulopathy, and hypertriglyceridemia. The pathogenesis of FHL is not known but the above clinical and laboratory findings are compatible...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::The value of a postremission treatment in acute myelogenous leukemia (AML), with alternating combinations of non-cross-resistant drugs, has been prospectively assessed. Of 515 evaluable patients, 347 (67.4%) entered into complete remission (CR), following induction treatment with daunorubicin (DNR), vincristine (VCR),...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-03-01 00:00:00
abstract::A microperfusion system containing a glass capillary, the inner surface of which is coated with a phospholipid bilayer containing tissue factor, was used to explore the requirement for factors VIIa and Xa in the complex formed with the lipoprotein-associated coagulation inhibitor (LACI). Various combinations of factor...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Recent findings that hemoglobin S gelation and sickling are pH-dependent and also influence oxygen affinity suggested that the red cells containing this hemoglobin variant might show an abnormal Bohr effect. We therefore studied the effects of pH variation on the in vitro oxygen affinity of whole blood from persons wi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a malignant disorder of the hematopoietic stem cell. It has been shown that normal stem cells coexist with malignant stem cells in the bone marrow of patients with chronic-phase CML. To characterize the primitive hematopoietic progenitor cells within CML marrow, CD34+DR- and CD34+DR+ ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Hematopoietic reconstitution following syngeneic bone marrow transplantation with graded doses of untreated and drug-treated bone marrow was studied in B6D2F1 mice. Granulocyte-macrophage colony-forming units (CFU-GM) and spleen colony-forming units (CFU-S) showed similar in vitro drug sensitivities. Both the speed of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::The mechanisms of tissue necrosis and vascular damage characteristics of certain Epstein-Barr virus (EBV)-associated lymphoproliferative disorders are unknown. The CXC chemokines interferon-gamma-inducible protein-10 (IP-10) and the monokine induced by interferon-gamma (Mig) caused tissue necrosis and vascular damage ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-11-15 00:00:00
abstract::The tyrosine kinase receptor fetal liver kinase 1 (Flk-1) plays a crucial role in vasculogenesis and angiogenesis, but its target genes remain elusive. Comparing Flk-1(+/+) with Flk-1(-/-) embryonic stem (ES) cells, we identified transcripts regulated by the vascular endothelial growth factor A (VEGF-A)/Flk-1 pathway ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-11-3827
更新日期:2004-06-15 00:00:00
abstract::Managing vitamin K antagonist (VKA) therapy is challenging in children because of a narrow therapeutic range and wide inter- and intra-individual variability in dose response. Only a few small studies have investigated the effect of nongenetic and genetic factors on the dose response to VKAs in children. In a cohort s...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-07-365502
更新日期:2012-01-19 00:00:00
abstract::Rabbit platelets were aggregated by adenosine diphosphate (ADP), allowed to deaggregate and then separated into density subpopulations by centrifugation through discontinuous Stractan density gradients. Although ADP causes little or no release of the contents of the amine storage granules of rabbit platelets, ADP caus...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::TLRs expressed on dendritic cells (DCs) differentially activate DCs when activated alone or in combination, inducing distinct cytokines and costimulatory molecules that influence T-cell responses. Defining the requirements of DCs to program T cells during priming to become memory rather than effector cells could enhan...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-317966
更新日期:2011-06-16 00:00:00
abstract::The abl oncogene is translocated from chromosome 9 to 22 in the creation of the Philadelphia (Ph1) chromosome. This article describes new translocation breakpoints identified in two patients with chronic myelogenous leukemia using Southern blotting and cloned human DNA probes from chromosome 9. The translocation break...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::The phagocyte nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) oxidase was functionally reconstituted in monkey kidney COS-7 cells by transfection of essential subunits, gp91(phox), p22(phox), p47(phox), and p67(phox). COS-7 cells express the essential small guanosine 5'-triphosphatase, Rac1. Transge...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.8.2653
更新日期:2002-04-15 00:00:00
abstract::Telomere erosion may be counteracted by telomerase. Here we explored telomere length (TL) and telomerase activity (TA) in primary cutaneous T-cell lymphoma (CTCL) by using quantitative polymerase chain reaction and interphase quantitative fluorescence in situ hybridization assays. Samples from patients with Sézary syn...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-05-500686
更新日期:2014-03-20 00:00:00
abstract::A defect in erythropoietin (EPO) production has been advocated as being the main cause of anemia presented at time of diagnosis or during treatment by adults with solid tumors. On the basis of this defect, anemic cancer patients, both adults and children, have been treated with recombinant human EPO (rHuEPO). To furth...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Soluble thrombomodulin plasma concentrations are elevated in steroid-resistant graft-versus-host disease (GVHD), implying endothelial hypofunctioning for thrombomodulin-dependent generation of activated protein C's (APC) anticoagulant, anti-inflammatory, and antiapoptotic functions. Recombinant thrombomodulin or APC a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019001259
更新日期:2019-08-29 00:00:00
abstract::Little is known about mechanisms involved in skin-specific homing of cutaneous T-cell lymphoma (CTCL). Chemokine/chemokine receptor interactions have been implicated in the homing of lymphoma cells to various tissue sites. We investigated tissue samples and tumor cell suspensions of patients with CD30(+) CTCL (n = 8) ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-02-0475
更新日期:2003-02-15 00:00:00
abstract::Myeloproliferative neoplasms (MPNs) are a group of blood cancers that arise following the sequential acquisition of genetic lesions in hematopoietic stem and progenitor cells (HSPCs). We identify mutational cooperation between Jak2V617F expression and Dnmt3a loss that drives progression from early-stage polycythemia v...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-04-846220
更新日期:2018-12-27 00:00:00
abstract::It has been shown that histamine induces early changes on endothelial cells (EC), such as a transient expression of P-selectin and secretion and/or surface expression of early mediators (eg, prostacyclin [PG1(2)], platelet-activating factor [PAF], and leukotriene B4 [LTB4]). However, delayed effects of histamine on EC...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::The interleukin-6 receptor (IL-6R)/signal transducer and activator of transcription 3 (STAT3) pathway contributes to the pathogenesis of multiple myeloma (MM) and protects MM cells from apoptosis. However, MM cells survive the IL-6R blockade if they are cocultured with bone marrow stromal cells (BMSCs), suggesting tha...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-04-1670
更新日期:2004-12-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is suspected to be a strong mortality determinant of hemolytic disorders. However, direct contribution of acute intravascular hemolysis to fatal PAH has not been investigated. The roles of nitric oxide (NO) insufficiency and platelet activation in hemolysis-associated fatal PAH ha...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-267112
更新日期:2010-09-02 00:00:00
abstract::Although recent data suggests that osteoblasts play a key role within the hematopoietic stem cell (HSC) niche, the mechanisms underpinning this remain to be fully defined. The studies described herein examine the role in hematopoiesis of Osteopontin (Opn), a multidomain, phosphorylated glycoprotein, synthesized by ost...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-11-4422
更新日期:2005-08-15 00:00:00
abstract::We have shown previously that the cause of anemia in healthy elderly subjects can usually not be identified. In this study, hematopoiesis was examined in 18 healthy elderly subjects with unexplained anemia and in 15 young and 15 healthy elderly individuals without anemia. No reduction in circulating testosterone was n...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) has potent physiologic and pathologic effects on a variety of cell types at subnanomolar concentrations. Platelets contain 40 times as much TGF-beta1 as other cells and secrete it as an inactive (latent) form in complex with latency-associated peptide (LAP), which is disulf...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-04-151753
更新日期:2008-11-01 00:00:00