Abstract:
:Wiskott-Aldrich syndrome (WAS) pediatric patients exhibit a deficiency in humoral immune memory. However, the mechanism by which Wiskott-Aldrich syndrome protein (WASP) regulates the differentiation and activation of memory B cells remains elusive. Here we examine the early activation events of memory B cells from the peripheral blood mononuclear cells of WAS patients and age-matched healthy controls (HCs) using total internal reflection fluorescence microscopy. In response to stimulation through the B-cell receptor (BCR), memory B cells from HCs showed significantly higher magnitudes of BCR clustering and cell spreading than naive B cells from the same individuals. This was associated with increases in CD19 recruitment to the BCR and the activation of its downstream signaling molecule Btk and decreases in FcγRIIB recruitment and the activation of its downstream molecule Src homology 2-containing inositol 5' phosphatase (SHIP). However, these enhanced signaling activities mediated by CD19 and Btk are blocked in memory B cells from WAS patients, whereas the activation of FcγRIIB and SHIP was increased. Although the expression levels of CD19, Btk, and FcγRIIB did not change between CD27(-) and CD27(+) B cells of HCs, the protein and mRNA levels of CD19 but not Btk and FcγRIIB were significantly reduced in both CD27(-) and CD27(+) B cells of WAS patients, compared with those of HCs. Overall, our study suggests that WASP is required for memory B-cell activation, promoting the activation by positive regulating CD19 transcription and CD19 recruitment to the BCR.
journal_name
Bloodjournal_title
Bloodauthors
Bai X,Zhang Y,Huang L,Wang J,Li W,Niu L,Jiang H,Dai R,Zhou L,Zhang Z,Miller H,Song W,Zhao X,Liu Cdoi
10.1182/blood-2016-03-703579subject
Has Abstractpub_date
2016-09-29 00:00:00pages
1723-34issue
13eissn
0006-4971issn
1528-0020pii
blood-2016-03-703579journal_volume
128pub_type
杂志文章相关文献
BLOOD文献大全abstract::Annexin V has phospholipid-binding capacity and plays a potent antithrombotic role. Recently, a C to T transition has been described in the Kozak region of this gene, affecting the nucleotide preceding the initiation ATG codon. We have developed a simple method to detect this genetic change, showing by analysis of 580...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2002-09-15 00:00:00
abstract::Activated neutrophils have the ability to upregulate the expression of many genes, in particular those encoding cytokines and chemokines, and to subsequently release the corresponding proteins. Although little is known to date concerning the regulation of gene transcription in neutrophils, it is noteworthy that many o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::The ataxia telangiectasia mutated (ATM)-interacting protein ATMIN mediates noncanonical ATM signaling in response to oxidative and replicative stress conditions. Like ATM, ATMIN can function as a tumor suppressor in the hematopoietic system: deletion of Atmin under the control of CD19-Cre results in B-cell lymphomas i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-09-672980
更新日期:2016-10-20 00:00:00
abstract::Retrovirus-mediated gene transfer into long-lived human pluripotent hematopoietic stem cells (HSCs) is a widely sought but elusive goal. A major problem is the quiescent nature of most HSCs, with the perceived requirement for ex vivo prestimulation in cytokines to induce stem cell cycling and allow stable gene integra...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Endothelial cell receptors for tissue plasminogen activator (t-PA) have been demonstrated recently, and we have sought to identify a region of the t-PA molecule involved in its interaction with these receptors on human umbilical vein endothelial cells. Of three monoclonal antibodies against various regions of t-PA, on...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::In this report we describe the production and characterization of a monoclonal antibody to the human promyelocytic leukemia cell line HL-60. The antibody, NC-2, is of the IgG1 subclass and precipitates a 50-Kd protein from 125I-labeled HL-60 cells. The antigen is insensitive to treatment with trypsin, papain, or neura...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::AML1-ETO (AE) is a fusion product of t(8;21) observed in 40% French-American-British M2 type of acute myeloid leukemia (AML). Clinical data suggest that Ras mutation is a frequent cooperating event in t(8;21) AML. Whether constitutively active Ras promotes leukemogenesis on the t(8;21) background has not been demonstr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-264119
更新日期:2011-02-17 00:00:00
abstract::The duration of immunodeficiency following marrow transplantation is not known. Questionnaires were used to study the infection rates in 72 patients surviving 20 to 30 years after marrow grafting. Furthermore, in 33 of the 72 patients and in 16 donors (siblings who originally donated the marrow) leukocyte subsets were...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.13.3505
更新日期:2001-12-15 00:00:00
abstract::McCullough and colleagues show that problems with cord banking still exist. To improve the results of cord transplantations, more attention should be given to ensuring quality standards rather than just collecting more units. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2009-06-226738
更新日期:2009-08-20 00:00:00
abstract::The efficacy and safety of zanolimumab in patients with refractory cutaneous T-cell lymphoma (CTCL) have been assessed in two phase 2, multicenter, prospective, open-label, uncontrolled clinical studies. Patients with treatment refractory CD4(+) CTCL (mycosis fungoides [MF], n = 38; Sézary syndrome [SS], n = 9) receiv...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2006-12-062877
更新日期:2007-06-01 00:00:00
abstract::Enhanced tolerance to combination chemotherapy has been cited as an ancillary benefit of staging laparotomy and splenectomy in Hodgkin's disease. Seventeen patients with Hodgkin's disease and 15 with non-Hodgkin's lymphoma were subjected to nontherapeutic splenectomy as part of the staging procedure prior to their ini...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-02-01 00:00:00
abstract::Two tumor necrosis factor receptors (TNFRs) with molecular weights of 55 kD (TNFR-p55) and 75 kD (TNFR-p75) have recently been identified and cloned. In previous studies, TNFR-p55 has been shown to exclusively mediate bidirectional effects of TNF-alpha on committed bone marrow granulocyte-macrophage progenitor cells, ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-02-15 00:00:00
abstract::For most children who relapse with acute lymphoblastic leukemia (ALL), the prognosis is poor, and there is a need for novel therapies to improve outcome. We screened samples from children with B-lineage ALL entered into the ALL-REZ BFM 2002 clinical trial (www.clinicaltrials.gov, #NCT00114348) for somatic mutations ac...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-04-531871
更新日期:2014-11-27 00:00:00
abstract::Migration to lymph nodes and secretion of cytokines are critical functions of mature dendritic cells (DCs); however, these 2 functions are not necessarily linked. This is the first report showing that quantitative differences in identical signaling pathways determine DC migration and cytokine secretion. Using differen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-12-4146
更新日期:2004-08-15 00:00:00
abstract::We evaluated the efficacy of umbilical cord blood (UCB) in the setting of a nonmyeloablative regimen consisting of fludarabine (200 mg/m2), cyclophosphamide (50 mg/kg), and a single fraction of total body irradiation (200 cGy) with cyclosporine and mycophenolate mofetil for posttransplantation immunoprophylaxis. The t...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2007-04-067215
更新日期:2007-10-15 00:00:00
abstract::Interactions between vascular endothelial cells and blood platelets have been investigated using a model microcirculation consisting of microcarrier beads colonized with human umbilical vein endothelial cells (HUVECs) and perfused with washed platelet suspensions. To simulate the effects of endothelial desquamation an...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract::Despite androgens and intensive supportive care, satisfactory survival in severe aplastic anemia remains at 20% or less. Histocompatible bone marrow transplantation can restore normal hematopoiesis in approximately 40% of similarly severe individuals. Delay of transplantation for 3 wk after diagnosis allows time for p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::We previously showed that incorporating target sequences for the hematopoietic-specific microRNA miR-142 into an antigen-encoding transgene prevents antigen expression in antigen-presenting cells (APCs). To determine whether this approach induces immunologic tolerance, we treated mice with a miR-142-regulated lentivir...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-04-214569
更新日期:2009-12-10 00:00:00
abstract::Donor lymphocyte infusions (DLIs) induce potent graft versus tumor (GVT) effects for relapsed chronic myelogenous leukemia (CML) after allogeneic stem cell transplantation (SCT) but are disappointing for other diseases. Disease resistance can occur if donor T cells are not appropriately activated in vivo. Ex vivo T-ce...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-08-3373
更新日期:2006-02-15 00:00:00
abstract::Professional antigen-presenting cells (APCs) are sentinel cells of the immune system that present antigen to T lymphocytes and mediate an appropriate immune response. It is therefore surprising that knowledge of the professional APCs in human lymph nodes is limited. Using 3-color immunohistochemistry, we have identifi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-06-165266
更新日期:2009-02-05 00:00:00
abstract::An immunotoxin specific for human T lymphocytes was prepared by coupling an IgG2a anti-CD3 murine monoclonal antibody (64.1) to purified ricin A chain (64.1-A). Treatment of blood mononuclear cells with this immunotoxin at a concentration of 1.7 X 10(-9) mol/L for two hours at 37 degrees C in the presence of 20 mmol/L...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Human polymorphonuclear leukocytes adhesion to endothelial cells during the early stage of inflammation leads to cell surface externalization of Annexin A1 (AnxA1), an effector of endogenous anti-inflammation. The antiadhesive properties of AnxA1 become operative to finely tune polymorphonuclear leukocytes transmigrat...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-02-270520
更新日期:2010-11-18 00:00:00
abstract::In this report, we show that the Src family nonreceptor protein tyrosine kinase (PTK) Lyn associates with aggregated IgA Fc receptor (Fc alpha R) in the monocytic cell line THP-1. Receptor aggregation and subsequent immunoprecipitation of receptor complexes with huIgA adsorbed to nitrocellulose particles shows that Ly...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-01-15 00:00:00
abstract::We have previously demonstrated that young adult DBA/2 (DBA) mice have more stem cells than C57BL/6 (B6) mice, as measured in a cobblestone area-forming cell (CAFC) assay using unfractionated marrow. To study the nature of this difference, we have now compared the proliferative fate of single, highly enriched Sca-1(+)...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-08-15 00:00:00
abstract::In this issue of Blood, the findings of Chakraborty et al and Emile et al support a model in which the mitogen-activated protein kinase (MAPK) and PI3K/AKT pathways are critical in the pathogenesis of 2 of the most common histiocytoses—Langerhans cell histiocytosis (LCH) and Erdheim-Chester disease (ECD)—whereas their...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2014-09-597765
更新日期:2014-11-06 00:00:00
abstract::[This corrects the article DOI: 10.1182/blood-2014-09-599423.]. ...
journal_title:Blood
pub_type: 杂志文章,已发布勘误
doi:10.1182/blood-2016-02-700625
更新日期:2016-03-24 00:00:00
abstract::Epinephrine causes platelet aggregation and secretion by interacting with alpha 2-adrenergic receptors on the platelet surface. Platelet aggregation requires the binding of fibrinogen to a specific receptor on the membrane glycoprotein IIb-IIIa complex. Although the IIb-IIIa complex is identifiable on the surface of r...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Somatic mutation in immunoglobulin variable (V) region genes occurs largely in the germinal center and, after neoplastic transformation, imprints V genes of B-cell tumors with the mutational history of the cell of origin. Recently, it has been found that chronic lymphocytic leukemia (CLL) consists of 2 subsets, each w...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::In this issue of Blood, Acharya and colleagues provide evidence for the role of angiogenesis in the pathophysiology of hemophilic joint disease. Is this the linchpin that unravels this important clinical condition or merely a cog in a not so stepwise process? ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2011-01-327312
更新日期:2011-02-24 00:00:00
abstract::Mantle cell lymphoma (MCL) is a mature B-cell neoplasm initially driven by CCND1 rearrangement with 2 molecular subtypes, conventional MCL (cMCL) and leukemic non-nodal MCL (nnMCL), that differ in their clinicobiological behavior. To identify the genetic and epigenetic alterations determining this diversity, we used w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020005289
更新日期:2020-09-17 00:00:00