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Nbeal2 interacts with Dock7, Sec16a, and Vac14.

Abstract：

:Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a, and Vac14 as interactors of Nbeal2. We show that GPS-causing mutations in its BEACH domain have profound and possible effects on the interaction with Dock7 and Vac14, respectively. Proximity ligation assays show that these 2 proteins are physically proximal to Nbeal2 in human megakaryocytes. In addition, we demonstrate that Nbeal2 is primarily localized in the cytoplasm and Dock7 on the membrane of or in α-granules. Interestingly, platelets from GPS cases and Nbeal2 -/- mice are almost devoid of Dock7, resulting in a profound dysregulation of its signaling pathway, leading to defective actin polymerization, platelet activation, and shape change. This study shows for the first time proteins interacting with Nbeal2 and points to the dysregulation of the canonical signaling pathway of Dock7 as a possible cause of the aberrant formation of platelets in GPS cases and Nbeal2-deficient mice.

journal_name

Blood

journal_title

Blood

authors

Mayer L,Jasztal M,Pardo M,Aguera de Haro S,Collins J,Bariana TK,Smethurst PA,Grassi L,Petersen R,Nurden P,Favier R,Yu L,Meacham S,Astle WJ,Choudhary J,Yue WW,Ouwehand WH,Guerrero JA

doi

10.1182/blood-2017-08-800359

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

1000-1011

issue

9

eissn

0006-4971

issn

1528-0020

pii

blood-2017-08-800359

journal_volume

131

pub_type

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