Abstract:
:Recent data suggest that CD8+ T-cell effector activity is an important component in the control of HIV replication in elite controllers (ECs). One critical element of CD8+ T-cell effector function and differentiation is the T-box transcription factor T-bet. In the present study, we assessed T-bet expression, together with the effector proteins perforin, granzyme A (Grz A), granzyme B (Grz B), and granulysin, in HIV-specific CD8+ T cells from ECs (n = 20), chronically infected progressors (CPs; n = 18), and highly active antiretroviral therapy (HAART)-suppressed individuals (n = 19). Compared with the other cohort groups, HIV-specific CD8+ T cells among ECs demonstrated a superior ability to express perforin and Grz B, but with no detectable difference in the levels of Grz A or granulysin. We also observed higher levels of T-bet in HIV-specific CD8+ T cells from ECs, with an ensuing positive correlation between T-bet and levels of both perforin and Grz B. Moreover, HIV-specific CD8+ T cells in ECs up-regulated T-bet to a greater extent than CPs after in vitro expansion, with concomitant up-regulation of perforin and Grz B. These results suggest that T-bet may play an important role in driving effector function, and its modulation may lead to enhanced effector activity against HIV.
journal_name
Bloodjournal_title
Bloodauthors
Hersperger AR,Martin JN,Shin LY,Sheth PM,Kovacs CM,Cosma GL,Makedonas G,Pereyra F,Walker BD,Kaul R,Deeks SG,Betts MRdoi
10.1182/blood-2010-12-322727subject
Has Abstractpub_date
2011-04-07 00:00:00pages
3799-808issue
14eissn
0006-4971issn
1528-0020pii
blood-2010-12-322727journal_volume
117pub_type
杂志文章相关文献
BLOOD文献大全abstract::In a previous multicenter phase III trial comparing peripheral blood stem cell transplantation (PBSCT) to bone marrow transplantation (BMT) from HLA-matched related donors, we found no statistically significant difference in the cumulative incidence of clinical extensive chronic graft-versus-host disease (GVHD) in the...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2002-01-0011
更新日期:2002-07-15 00:00:00
abstract::The rates of endocytosis, intracellular degradation, and cell-surface shedding of 125I-labeled monoclonal antibodies (MoAbs) HD-37 (anti-CD19), B1 (anti-CD20), MB-1 (anti-CD37), BC8 (anti-CD45), and DA4-4 (anti-mu) by B-lymphoma cells were compared by cellular radioimmunoassay, ultrastructural autoradiography, sodium ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::An inverse relationship between BCL-2 expression and cell cycle transition has been suggested by recent studies in murine models. To investigate the clinical relevance of these laboratory studies, a group of 116 paraffin-embedded non-Hodgkin's lymphoma (NHL) biopsy specimens (Working Formulation Groups D-H, and J) fro...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:
更新日期:1998-02-15 00:00:00
abstract::The effects of transforming growth factor-beta 1 (TGF-beta 1) on proliferation and hemoglobinization in K-562 cells, a human multipotential hematopoietic cell line, were studied. We found that TGF-beta 1 could induce hemoglobin accumulation in K-562 cells. Various clones were selected on the basis of the inducibility ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-11-15 00:00:00
abstract::Phosphoinositide 3-kinase gamma (PI3Kgamma) in neutrophils plays a critical role in the directed migration of these cells into inflamed tissues. In this study, we demonstrate the importance of the endothelial component of PI3Kgamma activity relative to its leukocyte counterpart in supporting neutrophil interactions wi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-01-0023
更新日期:2005-07-01 00:00:00
abstract::Fas ligand (FasL) and perforin pathways not only are the major mechanisms of T cell-mediated cytotoxicity but also are involved in homeostatic regulation of these T cells. In the present study, we tested whether CD8+ donor T cells that are deficient in both perforin and FasL (cytotoxic double deficient [cdd]) could in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-08-3036
更新日期:2005-03-01 00:00:00
abstract::Hereditary hemochromatosis is caused by mutations in the hereditary hemochromatosis protein (HFE), transferrin-receptor 2 (TfR2), hemojuvelin, hepcidin, or ferroportin genes. Hepcidin is a key iron regulator, which is secreted by the liver, and decreases serum iron levels by causing the down-regulation of the iron tra...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-09-245209
更新日期:2010-04-22 00:00:00
abstract::The cells of hematopoietic and vascular endothelial cell lineages are believed to share a common precursor, termed hemangioblast. However, the existence of a growth factor acting relatively specifically on hemangioblasts remains unclear. Here we report the identification of hemangiopoietin (HAPO), a novel growth facto...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-06-1825
更新日期:2004-06-15 00:00:00
abstract::It has been hypothesized that some of the irreversible microrheologic abnormalities of sickle red blood cell (RBC) membranes could result from autoxidative perturbation. To model this possibility, we used micromechanical manipulation to examine the static extensional rigidity and inelastic or plastic behavior of norma...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::The terminal differentiation of B cells into antibody-secreting cells (ASCs) is a critical component of adaptive immune responses. However, it is a very sensitive process, which dysfunctions lead to a great variety of lymphoproliferative neoplasia including germinal center-derived lymphomas. To better characterize the...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020005083
更新日期:2020-11-04 00:00:00
abstract::The transcription factor signal transducer and activator of transcription 5 (STAT5) fulfills essential roles in self-renewal in mouse and human hematopoietic stem cells (HSCs), and its persistent activation contributes to leukemic transformation, although little molecular insight into the underlying mechanisms has bee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-303669
更新日期:2011-03-24 00:00:00
abstract::The short-term beneficial effect of hydroxyurea (HU) in sickle cell disease (SCD) has been proven by randomized studies in children and adults. The Belgian registry of HU-treated SCD patients was created to evaluate its long-term efficacy and toxicity. The median follow-up of the 93 patients registered is 3.5 years; c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.11.3628
更新日期:2001-06-01 00:00:00
abstract::Approximately 50% of multi-transfused individuals become refractory to random donor platelets. Recent clinical data suggest that those patients receiving leukocyte-depleted blood products are less likely to become refractory to random donor platelets than recipients of non-leukocyte-depleted products. Leukocyte deplet...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::Approximately 20% of children with acute lymphoblastic leukemia (ALL) relapse because of failure to eradicate the disease. Current drug efficacy studies focus on reducing leukemia cell burden. However, if drugs have limited effects on leukemia-initiating cells (LICs), then these cells may expand and eventually cause r...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-08-448852
更新日期:2013-02-21 00:00:00
abstract::Although a large body of data on mobilization have yielded valuable clues, the mechanism(s) dictating egress of stem/progenitor cells during baseline hematopoiesis and after their mobilization are poorly understood. We have previously provided functional in vivo evidence that cytoadhesion molecules, specifically the b...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Human umbilical vein endothelial cells were analyzed for the presence of prothrombin, factor VII, protein C, and protein S in culture supernatants and cell extracts using specific radioimmunoassays. Only protein S was detected. Conditioned medium from 24-hour cultures and cell lysates contained 21.7 ng/mL and 88.8 ng/...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::Erythropoietin (Epo) binding to the Epo receptor (EpoR) elicits downstream signaling that is essential for red blood cell production. One important negative regulatory mechanism to terminate Epo signaling is Epo-induced EpoR endocytosis and degradation. Defects in this mechanism play a key role in the overproduction o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-05-506212
更新日期:2013-12-05 00:00:00
abstract::In a series of 153 children with T-cell malignancies enrolled in 2 consecutive European Organization for Research and Treatment of Cancer (EORTC) trials, we assessed the HOX11L2 expression and/or the presence of a t(5;14)(q35;q32). Additionally, in 138 of these patients, HOX11 expression and SIL-TAL rearrangement were...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2003-05-1495
更新日期:2004-01-15 00:00:00
abstract::Survivin, a member of the inhibitor of apoptosis protein family, is expressed in almost all types of malignancies, making this protein a useful tool for the development of broadly applicable vaccination therapies. We used a recently identified HLA-A2 binding peptide and dendritic cells (DCs) from healthy donors to ind...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-08-2554
更新日期:2003-07-15 00:00:00
abstract::Immunoglobulin class switching from IgM to IgG in response to peptides is generally T cell-dependent and vaccination in T cell-deficient individuals is inefficient. We show that a vaccine consisting of a dense array of peptides on liposomes induced peptide-specific IgG responses totally independent of T-cell help. Ind...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-02-413831
更新日期:2013-01-03 00:00:00
abstract::To gain further insights in the pathogenesis of herpesvirus pneumonia in allogeneic bone marrow transplant recipients, transplanted mice (B10.BR --> CBA) with graft-versus-host disease (GVHD) and control mice (transplanted mice without GVHD and normal CBA mice) were infected intranasally with herpes simplex virus type...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::We conducted a double-blind, randomized multicenter trial to determine whether the addition of mycophenolate mofetil (MMF) improves the efficacy of initial systemic treatment of chronic graft-versus-host disease (GVHD). The primary endpoint was resolution of chronic GVHD and withdrawal of all systemic treatment within...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2009-02-202937
更新日期:2009-05-21 00:00:00
abstract::Sickle red cell (SS RBC) adhesion is believed to contribute to the process of vaso-occlusion in sickle cell disease (SCD). We previously found that the LW RBC adhesion receptor can be activated by epinephrine to mediate SS RBC adhesion to endothelial alphavbeta3 integrin. To determine the contribution of LW activation...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-11-056101
更新日期:2007-10-01 00:00:00
abstract::Recently, the interleukin-2 receptor (IL-2R) was shown to be present on human neutrophils, and IL-2-neutrophil interactions are believed to be important in both tumor rejection and increased susceptibility to bacterial infections. Furthermore, neutrophils have been shown to synthesize host defense proteins, such as cy...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::To elucidate the biochemical basis of megaloblastic hematopoiesis, the cellular content and metabolism of deoxyribonucleoside triphosphates (dNTPs) were investigated using the bone marrow cells from nine patients with untreated vitamin B12 deficiency and one with folic acid deficiency. The marked imbalance among four ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::It has recently been shown that the Naka antigen, which is absent in 3% to 11% of Japanese blood donors, is expressed on platelet glycoprotein IV (GPIV; CD36) (Tomiyama et al, BLOOD, 75:684, 1990). In the present studies, flow cytometry was used to distinguish differences in the reactivity of Naka+ and Naka- platelets...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Protein Z is a vitamin K-dependent glycoprotein that plays a role in the regulation of coagulation. A nucleotide substitution of G by C in exon II of the protein Z gene, resulting in the replacement of Glu-30 with Gln (E30Q), and a G to A transition at the 79th nucleotide in intron F (IntF79G/A) were heterozygously id...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2250
更新日期:2005-04-15 00:00:00
abstract::The rapid recovery of hematopoiesis after allogeneic blood stem cell transplantation has been attributed to the quality and quantity of hematopoietic progenitors in the blood stem cell grafts from filgrastim-stimulated donors. To determine whether further stimulation with filgrastim after transplantation would affect ...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1182/blood.v97.11.3405
更新日期:2001-06-01 00:00:00
abstract::In this issue of Blood, Doyle et al provide evidence that knockout of the genes encoding the two most abundant eosinophil secondary granule proteins disrupts the normal differentiation of eosinophils from progenitors in the bone marrow, providing a novel strain of mice with a highly specific deficiency in eosinophilop...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2013-06-508507
更新日期:2013-08-01 00:00:00
abstract::Human T cells were studied with regard to the regulation of interleukin-4 (IL-4) and IL-3 gene expression. IL-4 and IL-3 mRNA were undetectable in unstimulated T cells. On activation with the lectin concanavalin A (Con A), both IL-4 and IL-3 mRNA were expressed. Accumulation of IL-4 mRNA peaked after 6 to 12 hours, wh...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00