Abstract:
:It has recently been shown that the Naka antigen, which is absent in 3% to 11% of Japanese blood donors, is expressed on platelet glycoprotein IV (GPIV; CD36) (Tomiyama et al, BLOOD, 75:684, 1990). In the present studies, flow cytometry was used to distinguish differences in the reactivity of Naka+ and Naka- platelets with both OKM5, a monoclonal antibody that recognizes an epitope on GPIV, and with polyclonal anti-GPIV antibody. OKM5 was also used to screen 871 platelet concentrates prepared from healthy US blood donors. Three of these showed markedly deficient binding of 125I-OKM5 or an incidence of 0.34%. Two of these donors were re-accessed and showed less than 1% binding of 125I-OKM5 as compared with 10,300 +/- 1,500 binding sites per platelet in controls (n = 4). Platelets from these two US donors were radiolabeled (125I, 3H) and compared with control platelets and with platelets from Japanese Naka+ and Naka- donors by crossed immunoelectrophoresis, protein blots, immunoprecipitation, and two-dimensional gel electrophoresis. GPIV could not be detected by any of these techniques in the Naka- platelets nor in the donors whose platelets showed deficient binding of OKM5. These results suggest that GPIV functions as an isoantigen rather than an alloantigen in immunizing Naka- platelet recipients. This is the first report of the absence of a major platelet membrane GP in healthy blood donors.
journal_name
Bloodjournal_title
Bloodauthors
Yamamoto N,Ikeda H,Tandon NN,Herman J,Tomiyama Y,Mitani T,Sekiguchi S,Lipsky R,Kralisz U,Jamieson GAsubject
Has Abstractpub_date
1990-11-01 00:00:00pages
1698-703issue
9eissn
0006-4971issn
1528-0020journal_volume
76pub_type
杂志文章相关文献
BLOOD文献大全abstract::Multiple lines of evidence indicate that thrombopoietin (TPO) substantially impacts the number of hematopoietic stem cells and progenitors of all myeloid lineages. Nevertheless, tpo knock-out mice (T(-)) display thrombocytopenia only; blood erythroid and neutrophil levels are normal despite 60% to 85% reductions in st...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.10.3573
更新日期:2002-05-15 00:00:00
abstract::Blockade of the CD40/CD154 signal is a potential immunomodulatory strategy for T-cell-mediated diseases. As a part of a phase 1, multicenter, dose-escalating trial of humanized monoclonal antibody to CD154 (IDEC-131/E6040) in patients with refractory immune thrombocytopenic purpura (ITP), the autoimmune response to gl...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1182/blood-2003-06-2167
更新日期:2004-02-15 00:00:00
abstract::Mechanisms involving the in vitro effect of rituximab in cells from 55 patients with B-cell lymphoproliferative disorders were investigated. No cytotoxic effect was observed when cells were incubated with rituximab alone, but in the presence of human AB serum rituximab induced complement-dependent cell death (R-CDC). ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.9.2771
更新日期:2001-11-01 00:00:00
abstract::The mouse Pim-1 gene encodes two cytoplasmic serine-threonine-specific protein kinases. The gene has been found to be activated (overexpressed) by retroviral insertion in hematopoietic tumors in mice. Transgenic mice that overexpress Pim-1 (E mu-Pim-1) have a low incidence of spontaneous T-cell lymphomas and an increa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::We evaluated a treatment strategy targeting both lymphoma cells (by rituximab) and the microenvironment (by thalidomide) in 16 patients with relapsed/refractory mantle cell lymphoma (MCL). Rituximab was administered at 375 mg/m(2) for 4 weekly doses concomitantly with thalidomide (200 mg daily, with a dose increment t...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2004-03-1091
更新日期:2004-10-15 00:00:00
abstract::Although the genes for four hematopoietic colony-stimulating factors (CSFs) have been cloned, neither the mechanism of the regulation of their production nor their cellular origins have been established with certainty. Monocytes are known to produce colony-stimulating and burst-promoting activities, as well as several...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Lymphocyte kinetic studies employing 51-chromium-labeled autologous lymphocytes were performed in nine normal volunteers in order to determine the effects of hydrocortisone administration on the recirculating versus the nonrecirculating intravascular lymphocyte pools. Following infusion of labeled cells, the recircula...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::The distinct combination of homing receptors such as selectins, chemokine receptors, and integrins directs the migration of lymphocytes throughout the body. Upon activation lymphocytes irreversibly switch their set of homing receptors, now guiding them to entirely different destinations. Here we report that exposure o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-12-064345
更新日期:2007-06-01 00:00:00
abstract::CD4+CD25+ regulatory T (Treg) cells are potent modulators of alloimmune responses. In murine models of allogeneic bone marrow transplantation, adoptive transfer of donor CD4+CD25+ Treg cells protects recipient mice from lethal acute graft-versus-host disease (aGVHD) induced by donor CD4+CD25- T cells. Here we examined...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-05-2044
更新日期:2005-03-01 00:00:00
abstract::Patients with immune thrombocytopenia (ITP) commonly have antiplatelet antibodies that cause thrombocytopenia through Fcγ receptors (FcγRs). Antibodies specific for FcγRs, designed to inhibit antibody-FcγR interaction, had been shown to improve ITP in refractory human patients. However, the development of such FcγR-sp...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-08-664656
更新日期:2016-01-07 00:00:00
abstract::In this issue of Blood, Rowley et al report that noncoding RNAs precisely regulate the messenger RNA (mRNA) profile in platelets. Interfering in this process using genetically engineered mice affects hemostatic and thrombotic functions of platelets. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2016-01-694893
更新日期:2016-04-07 00:00:00
abstract::Proinflammatory cytokines such as TNFα are elevated in patients with myeloproliferative neoplasms (MPN), but their contribution to disease pathogenesis is unknown. Here we reveal a central role for TNFα in promoting clonal dominance of JAK2(V617F) expressing cells in MPN. We show that JAK2(V617F) kinase regulates TNFα...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-348144
更新日期:2011-12-08 00:00:00
abstract::Immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with a complex pathogenesis, which includes both antibody- and T-cell-mediated effector mechanisms. Rituximab (an anti-human CD20 monoclonal antibody [mAb]) is one of the treatments for ITP and is known to deplete B cells but may also work by affecting t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-06-655126
更新日期:2016-02-11 00:00:00
abstract::In this issue of Blood, Pozzi et al demonstrate that removing an anionic cage promotes exposure of R169 thereby generating a protein C (PC) that is far more readily activated. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-05-427401
更新日期:2012-07-19 00:00:00
abstract::If bone marrow transplantation is to become widely applicable in the treatment of patients with leukemia and aplastic anemia, the necessity to have a perfectly histocompatible donor must be overcome. In an effort to define the roles of HL-A type and mixed lymphocyte culture (MLC) reactivity in the determination of suc...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::Using highly sensitive 2-site immunoradiometric assays, we examined the relationship between iron absorption from closed intestinal loops and transferrin and ferritin concentrations in isolated duodenal mucosal cells. As in prior studies, mucosal ferritin correlates inversely with iron absorption and directly with bod...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Whereas the chimeric type I anti-CD20 Ab rituximab has improved outcomes for patients with B-cell malignancies significantly, many patients with non-Hodgkin lymphoma (NHL) remain incurable. Obinutuzumab (GA101) is a glycoengineered, humanized anti-CD20 type II Ab that has demonstrated superior activity against type I ...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2012-01-404368
更新日期:2012-05-31 00:00:00
abstract::In this study, we randomly compared high doses of the tyrosine kinase inhibitor imatinib combined with reduced-intensity chemotherapy (arm A) to standard imatinib/hyperCVAD (cyclophosphamide/vincristine/doxorubicin/dexamethasone) therapy (arm B) in 268 adults (median age, 47 years) with Philadelphia chromosome-positiv...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2015-02-627935
更新日期:2015-06-11 00:00:00
abstract::The bone marrow (BM) microenvironment consists of extracellular-matrix and the cellular compartment including immune cells. Multiple myeloma (MM) cell and BM accessory cell interaction promotes MM survival via both cell-cell contact and cytokines. Immunomodulatory agents (IMiDs) target not only MM cells, but also MM c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-04-279893
更新日期:2010-10-28 00:00:00
abstract::Tumor cells from six patients with immunoglobulin G (IgG) multiple myeloma were analyzed for surface antigens, cytoplasmic paraprotein, morphology, and response to various culture conditions. The tumor marker was the paraprotein idiotype. Low numbers of tumor cells were found in the blood of most of the patients. In s...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-05-15 00:00:00
abstract::We previously found that the myeloid cell surface glycoprotein CD13 (gp150) is identical to aminopeptidase N (EC 3.4.11.2), a widely distributed membrane-bound, zinc-dependent metalloprotease with an extracellular enzymatic domain that cleaves N-terminal amino acid residues from oligopeptides (J Clin Invest 83:1299, 1...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-01-15 00:00:00
abstract::The engagement of inhibitory receptors specific for major histocompatibility complex class I (MHC-I) molecules educates natural killer (NK) cells, meaning the improvement of the response of activation receptors to subsequent stimulation. It is not known whether inhibitory MHC-I receptors educate only NK cells or wheth...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-01-331124
更新日期:2011-07-14 00:00:00
abstract::Congenital dyserythropoietic anemia type II (CDA-II) is an autosomal recessive disease characterized by anemia, jaundice, splenomegaly, and erythroblast multinuclearity. The natural history of the disease is unknown. The frequency, the relevance of complications, and the use of splenectomy are poorly defined. This stu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.4.1258
更新日期:2001-08-15 00:00:00
abstract::Defective function of dendritic cells (DC) in cancer has been recently described and may represent one of the mechanisms of tumor evasion from immune system control. We have previously shown in vitro that vascular endothelial growth factor (VEGF), produced by almost all tumors, is one of the tumor-derived factors resp...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::We have previously shown that intravenously injected peripheral blood (PB) or bone marrow (BM) cells from newly diagnosed chronic myeloid leukemia (CML) patients can engraft the BM of sublethally irradiated severe combined immunodeficient (SCID) mice. We now report engraftment results for chronic phase CML cells in no...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::We describe two assays to detect the action of colony-inhibiting cells. In the first assay, we used a simple density separation technique to remove dense neutrophils (PMN) from suspensions of blood and of bone marrow cells prior to culture in semisolid agar. Conditions were arranged to ensure that control suspensions ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::The purpose of this study was to determine the relative merits of idarubicin and daunorubicin in acute myeloid leukemia (AML) therapy. Thirty-two sites provided 214 previously untreated adults with AML aged 15 years or more who were randomized to receive for induction therapy cytarabine 100 mg/m2/d as a continuous 7-d...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1992-01-15 00:00:00
abstract::Fourteen patients with cytogenetic relapse of chronic myeloid leukemia (CML) after transplantation with unmanipulated bone marrow were treated with alpha-2a-interferon. There were eight men and six women, median age, 33 years. Twelve patients received marrow from a related allogeneic donor and two received marrow from...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::CD38 is highly and uniformly expressed on multiple myeloma (MM) cells, and at relatively low levels on normal lymphoid and myeloid cells, and in some tissues of nonhematopoietic origin. CD38 is a transmembrane glycoprotein with ectoenzymatic activity, and also functions as a receptor and adhesion molecule. Altogether,...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-06-740944
更新日期:2018-01-04 00:00:00
abstract::Little is known about the behavior of hematopoietic stem cells (HSCs) in primates because direct observations and competitive-repopulation assays are not feasible. Therefore, we used 2 different and independent experimental strategies, the tracking of transgene expression after retroviral-mediated gene transfer (N = 1...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-02-075382
更新日期:2007-09-15 00:00:00