Abstract:
:Phospholipase A(2) type IVA (IVAPLA(2)) is a cytosolic enzyme that on activation selectively releases arachidonic acid (AA) from cell membrane phospholipids. Both AA and lysophospholipid, products of the enzymic reaction, can function as signal transducers in cellular interactions. The enzyme is present in most cells, including polymorphs, eosinophils, and platelets. This study used affinity purification to extract IVAPLA(2) from red cell lysate prepared from leukocyte- and platelet-depleted human blood to overcome the masking effect of hemoglobin on Western blot detection. We show that IVAPLA(2) is present in red cells as a 90-kDa protein.
journal_name
Bloodjournal_title
Bloodauthors
Macdonald DJ,Boyle RM,Glen AC,Horrobin DFdoi
10.1182/blood-2002-09-2698subject
Has Abstractpub_date
2004-05-01 00:00:00pages
3562-4issue
9eissn
0006-4971issn
1528-0020pii
2002-09-2698journal_volume
103pub_type
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