Quantitation of adenosine-5'-triphosphate used for phosphoinositide metabolism in human erythrocytes.

Abstract:

:The human erythrocyte actively phosphorylates and dephosphorylates phosphatidylinositol present in the membrane in an apparent "futile cycle." Recent reports have proposed that this phosphorylation/dephosphorylation cycle is a significant consumer of adenosine-5'-triphosphate (ATP) in the erythrocyte. This study details two independent techniques for quantitating the ATP consumed by this phosphoinositide futile cycle. With the first technique a quasi-steady-state labeling of erythrocyte ATP with 32P-phosphate was obtained, and the rate of synthesis of 32P-phosphoinositides was then monitored. The second technique used a novel labeling strategy that allowed only ATP to be labeled with 32P; the transfer of 32P from ATP to phosphoinositides was then an independent measure of the ATP consumed for phosphoinositide synthesis. These two techniques documented that 0.5% to 1.0% of net ATP produced by the erythrocyte is used for phosphoinositide synthesis.

journal_name

Blood

journal_title

Blood

authors

Dale GL

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

1133-7

issue

5

eissn

0006-4971

issn

1528-0020

journal_volume

66

pub_type

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