Abstract:
:DNA of the avian hepadnavirus, duck hepatitis B virus, was found to be integrated at low abundance into the cellular DNA extracted from the livers of infected ducklings. The frequency of integration was estimated to be at least one viral genome per 10(3) to 10(4) cells by 6 days postinfection. The structures of virus-cell junctions determined by sequencing were compared with those of virus-virus junctions formed by nonhomologous recombination between the ends of linear viral DNA forms. This comparison allowed us to conclude that linear viral DNA was the preferential form used as an integration substrate. Potential factors promoting viral DNA integration during chronic infection are discussed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yang W,Summers Jdoi
10.1128/JVI.73.12.9710-9717.1999subject
Has Abstractpub_date
1999-12-01 00:00:00pages
9710-7issue
12eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::The major structural glycoprotein E2 of classical swine fever virus (CSFV) is responsible for eliciting neutralizing antibodies and conferring protective immunity. The current structural model of this protein predicts its surface-exposed region at the N terminus with a short stretch of the C-terminal residues spanning...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11619-11625.2000
更新日期:2000-12-01 00:00:00
abstract::The human genome contains more than half a million human endogenous retrovirus (HERV) long terminal repeats (LTRs) that can be regarded as mobile regulatory modules. Many of these HERV LTRs have been recruited during evolution as transcriptional control elements for cellular gene expression. We have cloned LTR sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00858-09
更新日期:2009-12-01 00:00:00
abstract::Measles virus has two glycoproteins. The larger glycoprotein (HA) is composed of 76,000-dalton subunits that are bound by disulfide bonds. The smaller glycoprotein (F) appears to contain a glucosamine-rich portion that is linked to an unglycosylated protein by disulfide bonds. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.2.687-692.1978
更新日期:1978-02-01 00:00:00
abstract::The DNA molecules from T7 bacteriophage and a recently obtained mutant form of T4D were studied. The DNA of this T4 mutant contains cytosine in place of all of the glucosylated hydroxymethylcytosines normally present in T4. Molecular weights were measured with an electron microscope technique, and sedimentation coeffi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.438-448.1980
更新日期:1980-01-01 00:00:00
abstract::An adult horse infected with a virulent, cell culture-adapted strain of equine infectious anemia virus (EIAV) developed cyclical thrombocytopenia in which the nadir of platelet counts coincided with peak febrile responses. In order to investigate the mechanism of thrombocytopenia during acute febrile episodes, four ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6242-6251.1991
更新日期:1991-11-01 00:00:00
abstract::The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch regio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7695-7705.1996
更新日期:1996-11-01 00:00:00
abstract::Recombinant adeno-associated virus (rAAV) vectors have been shown to be useful for efficient gene delivery to a variety of dividing and nondividing cells. Mechanisms responsible for the long-term, persistent expression of the rAAV transgene are not well understood. In this study we investigated the kinetics of rAAV-me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3555-3565.2000
更新日期:2000-04-01 00:00:00
abstract::DNA replication intermediates of three plasmids containing all or part of a modified Epstein-Barr virus cis-acting plasmid maintenance region (oriP) were examined to further investigate oriP function. Replication intermediates were analyzed in vivo and in vitro by neutral-neutral two-dimensional gel electrophoresis. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1739-1745.1993
更新日期:1993-03-01 00:00:00
abstract::Interferon regulatory factor 7 (IRF-7) is implicated in the regulation of Epstein-Barr virus (EBV) latency. EBV transforms primary B cells, and the major EBV oncoprotein, latent membrane protein 1 (LMP-1), is required for the process. LMP-1 both induces the expression of IRF-7 and activates the IRF-7 protein by phosph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.12987-12995.2004
更新日期:2004-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5'-CAGGTA-3', called ZV, located at nucleotides -17 to -12 relative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.867-877.2001
更新日期:2001-01-01 00:00:00
abstract::A recent publication indicated that overexpression of Axl, a cellular receptor that negatively regulates Toll-like receptor signaling, enhanced the entry of viruses pseudotyped with the glycoprotein of lymphocytic choriomeningitis virus (LCMV) in vitro. In testing the biological relevance of these observations, we fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
abstract::Retroviral vectors offer major advantages for gene transfer studies but have not been useful for producing proteins in large quantities. This deficiency has resulted in part from interference to superinfection, which limits the numbers of active proviruses in cells. Recently, we found that these vectors amplify when t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3500-3508.1990
更新日期:1990-07-01 00:00:00
abstract::The nonsegmented negative-strand RNA viruses (order Mononegavirales) include many important human pathogens. The order of their genes, which is highly conserved, is the major determinant of the relative levels of gene expression, since genes that are close to the single promoter site at the 3' end of the viral genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4705-4712.1999
更新日期:1999-06-01 00:00:00
abstract::We show here that the white spot syndrome virus (WSSV) immediate-early protein IE1 interacts with the Penaeus monodon TATA box-binding protein (PmTBP) and that this protein-protein interaction occurs in the absence of any other viral or cellular proteins or nucleic acids, both in vitro and in vivo. Mapping studies usi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02433-10
更新日期:2011-07-01 00:00:00
abstract::Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization. Three host cell molecules have been reported to be important entry factors or receptors for HCV internalizat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02286-07
更新日期:2008-05-01 00:00:00
abstract::Hepadnaviruses contain a relaxed circular DNA genome (RC-DNA) with discontinuities in both strands. Upon infectious entry into a host cell, this genome is converted into a covalently closed superhelical form (CCC-DNA), which later serves as the template for transcription. Here we examined whether the viral polymerase ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4867-4874.1993
更新日期:1993-08-01 00:00:00
abstract::The susceptibility to tumors induced by raf and raf/myc retroviruses was investigated in BALB/c, C57BL/6, (BALB/c x C57BL/6)F1 and (BALB/c x C57BL/6) backcross mice. Newborn mice were susceptible to neoplasms generated by both viruses, but resistance to raf-induced leukemia developed rapidly in all mice as they mature...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2411-2414.1989
更新日期:1989-05-01 00:00:00
abstract::CD8+ T cells were previously shown to be important in preventing lymphoproliferation and immunodeficiency following infection of murine AIDS (MAIDS)-resistant mice with the LP-BM5 mixture of murine leukemia viruses. To further evaluate the mechanisms contributing to MAIDS resistance, we studied mice lacking CD8+ T cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1808-1813.1997
更新日期:1997-03-01 00:00:00
abstract::Cell-mediated immunity depends in part on appropriate migration and localization of cytotoxic T lymphocytes (CTL), a process regulated by chemokines and adhesion molecules. Many viruses, including human immunodeficiency virus type 1 (HIV-1), encode chemotactically active proteins, suggesting that dysregulation of immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.5184-5193.2004
更新日期:2004-05-01 00:00:00
abstract::The vaccinia virus H2R gene (VACWR 100) is conserved in all sequenced members of the poxvirus family and encodes a protein with a predicted transmembrane domain and four invariant cysteines. A recombinant vaccinia virus, in which expression of the H2 protein is stringently regulated, was unable to replicate without in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4744-4754.2005
更新日期:2005-04-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus termed SARS-CoV. We and others have previously shown that the replication of SARS-CoV can be suppressed by exogenously added interferon (IFN), a cytokine which is normally synthesized by cells as a reaction to virus infection. Here, we demonstra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2079-2086.2005
更新日期:2005-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus persists. Polymorphisms within known antigenic sites result in loss of immune recognition and can be positively selected. Amino acid variation outside known HLA class I restricted epitopes can also enable immune escape by interf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00968-06
更新日期:2007-02-01 00:00:00
abstract::Embryonic stem cells are subjected to a dynamic genome regulation during development. Here we report that the ectopic lentiviral transgenes are quickly silenced in murine embryonic carcinoma P19 cells. The silencing was correlated with CpG hypermethylation in the transgene promoter. Using high-resolution sodium bisulf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13497-13508.2005
更新日期:2005-11-01 00:00:00
abstract::Human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. To gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. We show that for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6533-6540.2003
更新日期:2003-06-01 00:00:00
abstract::Flock house virus is a small insect virus with a bipartite RNA genome consisting of RNA1 and RNA2. RNA3 is a subgenomic element encoded by RNA1, the genomic segment required for viral RNA synthesis (T. M. Gallagher, P. D. Friesen, and R. R. Rueckert, J. Virol. 46:481-489, 1983). Synthesis of RNA3 is strongly inhibited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2716-2722.1993
更新日期:1993-05-01 00:00:00
abstract::The Gag proteins of retroviruses are the only viral products required for the release of membrane-enclosed particles by budding from the host cell. Particles released when these proteins are expressed alone are identical to authentic virions in their rates of budding, proteolytic processing, and core morphology, as we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.564-577.1998
更新日期:1998-01-01 00:00:00
abstract::Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11301-11311.2002
更新日期:2002-11-01 00:00:00
abstract::A vaccine regimen that elicits broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine research. In this study, we assessed the immunogenicity of the CAP256 superinfecting viral envelope (CAP256 SU) protein delivered by modified vaccinia virus Ankara (MVA) and DNA vaccines in different prime-boost com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
更新日期:2019-04-03 00:00:00
abstract::The synthesis of minus-strand RNA was studied in cell cultures infected with the heat-resistant strain of Sindbis virus and with temperature-sensitive (ts) belonging to complementation groups A, B, F, and G, all of which exhibited an RNA-negative (RNA-) phenotype when infection was initiated and maintained at 39 degre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.348-358.1981
更新日期:1981-08-01 00:00:00
abstract::We have used Northern blot hybridization to study the accumulation of specific cellular mRNAs in Vero cells infected with herpes simplex virus (HSV) type 1 or type 2. HSV-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone H3 and H4 mRNAs, though not all at the same rate. H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.601-610.1985
更新日期:1985-09-01 00:00:00