Region-dependent G-protein activation by mu-, delta 1- and delta 2-opioid receptor agonists in the brain: comparison between the midbrain and forebrain.

abstract：:Two male adult rhesus monkeys were used and caged individually. The room was temperature-controlled having a light-dark period of 12/12 hours. The animals were rapidly immobilized and immediately anesthetized with ketamine i. m. (10 mg/kg of body weight). They were bled four times at 15, 30, 45 and 60 mins after the k...

journal_title：Life sciences

authors： Mora OA,García Castiñeiras S,Ezquerra C,Guisado S

更新日期：1991-01-01 00:00:00

abstract：:The effects of chronic treatment with piracetam (100 mg/Kg) plus choline (100 mg/Kg) on rabbits' hippocampal electrical activity and behavior have been studied. Animals were exposed to a neutral environment, in the absence of any external stimulus, and then to an object and a stuffed sparrow-hawk. Results show a drug ...

journal_title：Life sciences

authors： Fontani G,Grazzi F,Meucci M

更新日期：1984-09-10 00:00:00

abstract：:This report documents studies on the spontaneous reactivation of human erythrocyte acetylcholinesterase and human serum butyrylcholinesterase following inhibition by organophosphinate esters. The spontaneous reactivation reactions were carried out at 26.0 degrees C in 0.10 M phosphate buffer of pH 7.6. Based upon resu...

journal_title：Life sciences

authors： Lawson MA,Lieske CN,Fox-Talbot MK,Meyer HG

更新日期：1985-05-06 00:00:00

abstract：:Free radicals induced by cerebral ischemia-reperfusion injury can trigger lipid peroxidation, leading to the production of malondialdehyde (MDA) and 4-hydroxy-2(E)-nonenal (4-HNE). Post-ischemia electroacupuncture (EA) therapy was able to reduce extent of lipid peroxidation. However, the effect of pre-ischemic EA ther...

journal_title：Life sciences

authors： Siu FK,Lo SC,Leung MC

更新日期：2004-07-30 00:00:00

abstract：AIMS:Pulmonary hypertension (PH) is a proliferative disorder characterized by enhanced proliferation and suppressed apoptosis of intrapulmonary vascular smooth muscle cells. Recently, network-based bioinformatics have identified let-7 family, a tumor suppressive microRNA, regulate multiple interacting targets relevant ...

journal_title：Life sciences

authors： Zhang WF,Xiong YW,Zhu TT,Xiong AZ,Bao HH,Cheng XS

更新日期：2017-02-01 00:00:00

abstract：AIMS:We recently reported that acute exposure to nicotine vasodilates the renal vasculature of male rats via facilitation of endothelial nitric oxide synthase (eNOS). In this study, we investigated whether this effect of nicotine is sexually dimorphic and the role of estrogen in modulating the nicotine effect. MAIN ME...

journal_title：Life sciences

authors： el-Mas MM,el-Gowilly SM,Gohar EY,Ghazal AR,Abdel-Rahman AA

更新日期：2011-01-17 00:00:00

abstract：AIMS:Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, has been reported to be a novel marker for the progression of chronic kidney disease (CKD). We have recently found that accumulation of ADMA could trigger peritubular capillary loss, thus contributing to tubulointerstitial ischemia ...

journal_title：Life sciences

authors： Ueda S,Yamagishi S,Matsumoto Y,Kaida Y,Fujimi-Hayashida A,Koike K,Tanaka H,Fukami K,Okuda S

更新日期：2009-06-05 00:00:00

abstract：:Tissue-type plasminogen activator (tPA) is a key enzyme in the fibrinolysis system and the regulation of its expression has been extensively studied in cultured vascular endothelial cells. Many kinds of supplements including growth factors are needed, however, to keep endothelial cells viable, which leads the culture ...

journal_title：Life sciences

authors： Yoshino A,Suzuki K,Urano T,Aoki K,Takada Y,Kazui T,Takada A

更新日期：2002-02-08 00:00:00

abstract：:Our recent report demonstrated that apoptosis-specific autoantibodies against granzyme B-induced cleavage fragments of SS-B (La) were found in the sera from patients with primary Sjögren's syndrome. The objective of this study was identified by the intracellular redistribution of La autoantigen during granzyme B-induc...

journal_title：Life sciences

authors： Huang M,Ida H,Arima K,Nakamura H,Aramaki T,Fujikawa K,Tamai M,Kamachi M,Kawakami A,Yamasaki H,Origuchi T,Eguchi K

更新日期：2007-10-27 00:00:00

abstract：:The binding of different gangliosides to rat T-helper lymphocytes was characterized under conditions that decrease CD4 expression on different mammalian T-helper lymphocytes. Saturation binding by monosialylated [3H]-GM1 to rat T-lymphocytes was time- and temperature-dependent, had a dissociation constant (KD) of 2.2 ...

journal_title：Life sciences

authors： Morrison WJ,Offner H,Vandenbark AA

更新日期：1989-01-01 00:00:00

abstract：:Cumulative damage in cells from aged people could lead to a greater fragility against acute oxidative stress. The effects of acute oxidative stress on cell viability, cAMP and cGMP concentrations, and protein synthesis rates were studied in lymphocytes from 25 young and 26 elderly subjects. Lymphocytes were exposed to...

journal_title：Life sciences

authors： Lopez-Hellin J,Garcia-Arumi E,Schwartz S

更新日期：1998-01-01 00:00:00

abstract：:We investigated the effects of VA-045, an apovincaminic acid derivative, on isolated blood vessels. VA-045 (10(-7)-10(-5) M) and vinpocetine (10(-7)-10(-5) M) inhibited the 64 mM KCl-induced and 10(-6)M norepinephrine (NE)-induced contraction of rat aortic strips. VA-045 (10(-7)-10(-4) M) and vinpocetine (10(-7)-10(-4...

journal_title：Life sciences

authors： Miyata N,Yamaura H,Tanaka M,Muramatsu M,Tsuchida K,Okuyama S,Otomo S

更新日期：1993-01-01 00:00:00

abstract：:We describe here a nonpeptide neuropeptide Y Y1 receptor antagonist, 2,4-dioxo-1,5-bis(2-oxo-2-orthotolyl-ethyl)-3-[3-[3-([3-[3-(3-p iperidin-1-ylmethyl-phenoxy)-propylcarbamoyl]-propyl]-car bamoyloxymethyl)-phenyl]-ureido]-2,3,4,5-tetrahydro-1H-benzo[b][1,4]diaz epine (Compound 1), which was previously synthesized as...

journal_title：Life sciences

authors： Shigeri Y,Ishikawa M,Ishihara Y,Fujimoto M

更新日期：1998-01-01 00:00:00

abstract：:The aim of this study is to investigate the effect of three cucurbitacins (Cuc) E, D and I on the bilirubin-albumin binding, both in human serum albumin (HSA) and in plasma. Bilirubin-HSA solution and plasma free of cucurbitacins were prepared as well as others containing serial concentrations of cucurbitacins. The co...

journal_title：Life sciences

authors： Greige-Gerges H,Abou Khalil R,Chahine R,Haddad C,Harb W,Ouaini N

更新日期：2007-01-16 00:00:00

abstract：AIMS:Transplantation of bone marrow mononuclear cells (BMMCs) exerts neuroprotection against cerebral ischemia. We examined the therapeutic timepoint of allogeneic BMMC transplantation in a rat model of focal cerebral ischemia, and determined the effects of repeated transplantation outside the therapeutic window. MAIN...

journal_title：Life sciences

authors： Kamiya F,Ueda M,Nito C,Kamiya N,Inaba T,Suda S,Saito T,Muraga K,Katayama Y

更新日期：2014-01-24 00:00:00

abstract：:Previous in vitro evidence suggests that adrenaline causes K influx in skeletal muscle by stimulating a ouabain sensitive Na/K ATPase membrane pump. However in rabbits, adrenaline induced hypokalaemia was not significantly altered by pretreatment with digoxin (50 micrograms/kg). Rats were infused with adrenaline or sa...

journal_title：Life sciences

authors： Struthers AD,Davies DL,Harland D,Price JS,Brown RA,Quigley C,Brown MJ

更新日期：1987-01-05 00:00:00

abstract：AIMS:Cisplatin (CDDP) is a potent anticancer agent, but severe renal toxicity can limit its use. We investigated the protective effect of cepharanthin (CE), a biscoclaurin alkaloid, on the renal toxicity of CDDP. MAIN METHODS:Mice were given CDDP along with CE. Effects of CE on CDDP toxicity were investigated by assay...

journal_title：Life sciences

authors： Sogawa N,Hirai K,Sogawa C,Ohyama K,Miyazaki I,Tsukamoto G,Asanuma M,Sasaki A,Kitayama S

更新日期：2013-04-09 00:00:00

abstract：AIMS:Parathyroid hormone-related protein (PTHrP) is a peptide growth factor produced in a wide range of tissues from brain and parathyroid, to kidney and uterus. The purpose of this study was to determine whether the adrenal cortical hormones, hydrocortisone (cortisol), modulate PTHrP expression and glucocorticoid rece...

journal_title：Life sciences

authors： Yoo YM,Baek MG,Jung EM,Yang H,Choi KC,Yu FH,Jeung EB

更新日期：2011-10-24 00:00:00

abstract：:Kappa (kappa) opioid agonists induce a water diuresis and inhibit vasopressin (AVP) secretion. Hypothalamic and neurohypophysial sites have both been implicated in the response. The present study was designed to ascertain if kappa-agonist inhibition of osmotically-stimulated AVP secretion is associated with parallel c...

journal_title：Life sciences

authors： Rossi NF,Kim JK,Summers SN,Schrier RW

更新日期：1997-01-01 00:00:00

abstract：:Anandamide (arachidonylethanolamide), isolated from porcine brain, has been shown to bind to the cannabinoid receptor and also to produce cannabimimetic activity in pharmacological assays. This study examined structure-activity relationships in alkylated anandamide analogs. The analogs were evaluated for their ability...

journal_title：Life sciences

authors： Adams IB,Ryan W,Singer M,Razdan RK,Compton DR,Martin BR

更新日期：1995-01-01 00:00:00

abstract：:Trifluoperazine (5-200 microM) stimulated the release of 3H-NE from isolated whole pineal glands in a dose dependent manner. Trifluoperazine-induced release was not dependent on extracellular Ca++, whereas 60 mM K+-evoked release was attenuated in the presence of EGTA and zero Ca++ Krebs. 60 mM K+ and 50 microM triflu...

journal_title：Life sciences

authors： Yurko KA,Quenzer LF

更新日期：1986-04-14 00:00:00

abstract：:We studied the effect of thapsigargin on intracellular calcium levels ([Ca2+]i) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. Thapsigargin elevated [Ca2+]i dose dependently with an EC50 of approximately 0.15 microM. The Ca2+ signal consisted of a slow rise, a gradual decay and a plateau. De...

journal_title：Life sciences

authors： Jan CR,Ho CM,Wu SN,Tseng CJ

更新日期：1999-01-01 00:00:00

abstract：:It has been reported that naloxone, an opioid antagonist, has antiarrhythmic activity in vivo. In Langendorff perfused rat hearts, we found that ischemia-reperfusion-induced ventricular tachyarrhythmia reverted to normal sinus rhythm after the treatment with naloxone (3 approximately 10 microM). The method of voltage ...

journal_title：Life sciences

authors： Hung CF,Wu MH,Tsai CH,Chu SH,Chi JF,Su MJ

更新日期：1998-01-01 00:00:00

abstract：:Artemisinin is a promising and a potent antimalarial drug, which meets the dual challenge posed by drug-resistant parasites and rapid progression of malarial illness. This review article focuses on the progress achieved during the last years in the production of artemisinin from Artemisia annua. The structure, biosynt...

journal_title：Life sciences

authors： Dhingra V,Vishweshwar Rao K,Lakshmi Narasu M

更新日期：2000-01-01 00:00:00

abstract：:Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibite...

journal_title：Life sciences

authors： Liew HC,Khoo HE,Moore PK,Bhatia M,Lu J,Moochhala SM

更新日期：2007-04-10 00:00:00

abstract：:Plasma 7B2-immunoreactivity (7B2-IR) concentrations in umbilical artery (UA), umbilical vein (UV) and maternal vein (MV) were measured by RIA at the time of obstetrical delivery at term. Plasma 7B2-IR concentrations (Mean +/- SEM) in UA (N = 12), UV (N = 16) and MV (N = 16) were 725 +/- 69, 699 +/- 64 and 116 +/- 4.5 ...

journal_title：Life sciences

authors： Iguchi H,Natori S,Nawata H,Kato K,Ibayashi H,Chan JS,Seidah NG,Chretien M

更新日期：1987-10-19 00:00:00

abstract：:Macrophages and osteoclasts derive from related cell lines. In osteopetrotic mutants the function of osteoclasts is greatly reduced compared to that in normal animals or children and macrophage function is variably affected depending upon the mutation. To further explore macrophage function in osteopetrosis we examine...

journal_title：Life sciences

authors： Popoff SN,Coderre JA,Marks SC Jr,Stock JL

更新日期：1989-01-01 00:00:00

abstract：:We have previously demonstrated that isatin (indole-2,3 dione), an endogenous compound widely distributed in mammalian tissues and body fluids, effectively inhibits atrial natriuretic peptide (ANP) receptor binding and ANP-stimulated guanylyl cyclase activity of rat membrane preparations. In the present study the effe...

journal_title：Life sciences

authors： Medvede AE,Abakumova OYu,Podobed OV,Tsvetkova TA,Sandler M,Glover V

更新日期：2001-08-31 00:00:00

abstract：:Morphine and the enkephalin-like peptide FK 33-824 given i.p. to rats potently inhibit gastrointestinal transit of a charcoal test meal, the doses inhibiting transit by 50% being respectively about 10 and 1 microgram/kg. These intestinal effects are opiate-specific, locally elicited and presumably involve the same act...

journal_title：Life sciences

authors： Manara L,Bianchi G,Fiocchi R,Notarnicola A,Peracchia F,Tavani A

更新日期：1982-09-20 00:00:00

abstract：:The immunosuppressive effects of microcolin A, a lipopeptide extracted from the marine blue green alga Lyngbya majuscula were investigated. Microcolin A suppressed concanavalin A (IC50 = 5.8 nM), phytohemagglutinin (IC50 = 12.5 nM) and lipopolysaccharide (IC50 = 8.0 nM) induced proliferation of murine splenocytes. Mix...

journal_title：Life sciences

authors： Zhang LH,Longley RE,Koehn FE

更新日期：1997-01-01 00:00:00