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Detection of membrane associated thioredoxin on human cell lines.

Abstract：

:Thioredoxin (TRX) is a ubiquitous dithiol-oxidoreduction enzyme broadly expressed in cells from prokaryote to eukaryote organisms. Human thioredoxin (human TRX) gene, previously cloned in our laboratory, codes for a 12-kDa protein found in the culture supernatant of several hemopoietic human cell lines. This protein is secreted by a nonclassical pathway. The role of the secreted enzyme as a signalling soluble mediator was demonstrated, but nothing is known about a membrane associated form of thioredoxin which could be involved in cell/cell contacts and accessory signal function. Thus, we performed experiments to determine if human TRX is also expressed at the cell surface. We report here positive results based upon indirect immunofluorescence flow cytometric analysis of different human cell lines (HeLa, U 937, Jurkat, 3B6, Daudi and Raji) using a cross reactive sheep anti E. coli TRX polyclonal antibody demonstrating a significant expression of human TRX at the surface of human cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wollman EE,Kahan A,Fradelizi D

doi

10.1006/bbrc.1996.6015

subject

Has Abstract

pub_date

1997-01-23 00:00:00

pages

602-6

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)96015-5

journal_volume

230

pub_type

杂志文章

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