Immunohistochemical evaluation of TGF-beta and extracellular matrix proteins expression in rat squamous cell carcinomas.

Abstract:

OBJECTIVE:The aim of this study was to establish a possible association between the degree of differentiation of squamous cell carcinomas (SCC) derived from rat oral tissues treated in vivo with carcinogen 4NQO, and the expression of TGF-beta on epithelial cells and the distribution of extracellular matrix proteins (laminin-collagen type IV). MATERIALS AND METHODS:A parent tumor showing a spectrum of differentiation was used to establish clonal subpopulations that formed differentiated SCC and undifferentiated (spindle cell phenotype) tumours following transplantation to athymic mice. RESULTS:Immunohistological findings revealed the absence of TGF-beta staining on epithelial cells and extracellular matrix proteins in spindle cell tumours. In contrast, staining of SCC revealed a significant number of TGF-beta positive cells and the presence of extracellular matrix proteins. CONCLUSIONS:The findings suggested that there is a positive correlation between histological differentiation, TGF-beta expression and the elaboration of extracellular matrix proteins.

journal_name

Oral Dis

journal_title

Oral diseases

authors

Poulopoulos AK,Kayavis IG,Papanayotou P

doi

10.1111/j.1601-0825.1996.tb00198.x

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

18-24

issue

1

eissn

1354-523X

issn

1601-0825

journal_volume

2

pub_type

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