Abstract：
:Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Specific translation products were absent in mutants and natural isolates unable to produce xanthomegnin metabolites. One mutant (TSM 73) did not produce several of these translation products, indicating that a mutation in a regulatory gene involved in xanthomegnin production had occurred.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Nicolaisen M,Sandal T,Frisvad JC,Rossen Ldoi
10.1016/S0944-5013(96)80026-7subject
Has Abstractpub_date
1996-08-01 00:00:00pages
285-90issue
3eissn
0944-5013issn
1618-0623pii
S0944-5013(96)80026-7journal_volume
151pub_type
杂志文章abstract：:Although reductive soil disinfestation (RSD) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after RSD remains poorly understood. MiSeq pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis were performed to investigate the changes ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.08.004
更新日期：2015-12-01 00:00:00
abstract：:The genes that encode the alpha and beta subunits of protocatechuate 3,4-dioxygenase (3,4-PCD [EC 1.13.11.3]) were cloned from a Pseudomonas marginata genomic library. These genes pcaG and pcaH, were found when screening the library for hydrolase genes. The two open reading frames of the PCD genes could be identified ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(96)80004-8
更新日期：1996-12-01 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in comple...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.08.006
更新日期：2016-11-01 00:00:00
abstract：:Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.09.010
更新日期：2019-01-01 00:00:00
abstract：:By chemical permeabilization of E. coli cells with detergents and membrane active peptides considerable amounts of the periplasmic anti-PC-miniantibodies can be released. Releases of active miniantibodies of 31%, respectively of 38%, were obtained by exposing low cell density suspensions to nonionic detergents like Tr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80056-0
更新日期：1997-12-01 00:00:00
abstract：:Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the s...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.02.007
更新日期：2011-12-20 00:00:00
abstract：:Glycosyl hydrolase (GH) family 18 chitinases (Chi) and family 33 chitin binding proteins (CBPs) from Bacillus thuringiensis serovar kurstaki (BtChi and BtCBP), B. licheniformis DSM13 (BliChi and BliCBP) and Serratia proteamaculans 568 (SpChiB and SpCBP21) were used to study the efficiency and synergistic action of BtC...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.01.006
更新日期：2013-08-25 00:00:00
abstract：:The disordered arrangement of flagella biosynthetic genes, combined with a simplified regulatory mechanism, has made elucidating the process of Campylobacter jejuni flagellation difficult. FlhF is a recently identified element that controls the assembly of the flagella, although its function mechanism and regulatory p...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.12.006
更新日期：2018-03-01 00:00:00
abstract：:Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.06.001
更新日期：2016-11-01 00:00:00
abstract：:In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella mel...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.012
更新日期：2008-01-01 00:00:00
abstract：:Eight ectomycorrhizal fungal isolates of Pisolithus associated with Eucalyptus species in different parts of India were collected and the genetic variability of these isolates was studied by ITS-RFLP and ITS sequencing. All the isolates showed same RFLP patterns with each restriction enzyme, indicating all these isola...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.03.003
更新日期：2004-01-01 00:00:00
abstract：:The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.08.016
更新日期：2018-01-01 00:00:00
abstract：:Outer membrane protein U (OmpU) is a major porin from Vibrio alginolyticus and has been considered a vaccine candidate against infection by V. alginolyticus. After pre-incubated with polyclonal antibody against rOmpU, V. alginolyticus showed a 78% decrease in extracellular iron level, suggesting that interruption of O...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126350
更新日期：2020-01-01 00:00:00
abstract：:Bacteria of the genus Agrobacterium can transfer a portion of their Ti plasmid (T-DNA) in complex with the VirE2 and VirD2 proteins into the plant-cell nucleus and cause it to be integrated in the host-cell chromosomes. The mechanism of T-DNA transfer across the plant-cell membrane and cytoplasm is unknown. The aim of...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.09.012
更新日期：2005-01-01 00:00:00
abstract：:The bacterium Helicobacter pylori infects more than 50% of the world population and causes several gastroduodenal diseases, including gastric cancer. Nevertheless, we still need to explore some protein interactions that may be involved in pathogenesis. MreB, an actin homolog, showed some special characteristics in pre...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.008
更新日期：2017-08-01 00:00:00
abstract：:Freeze-drying technology has been widely considered for decades as a suitable technique to preserve microorganisms. However, protective agents must be added prior to freeze drying to improve the survival and storage stability of the bacteria. The objective of our study was to evaluate the effect of a new protectant me...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126454
更新日期：2020-06-01 00:00:00
abstract：:Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The ex...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.08.004
更新日期：2015-01-01 00:00:00
abstract：:Cathepsin L (MrCathL) was identified from a constructed cDNA library of freshwater prawn Macrobrachium rosenbergii. MrCathL full-length cDNA is 1161 base pairs (bp) with an ORF of 1026bp which encodes a polypeptide of 342 amino acid (aa) long. The eukaryotic cysteine proteases, histidine and asparagine active site res...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.007
更新日期：2013-11-07 00:00:00
abstract：:Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytic...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.001
更新日期：2008-01-01 00:00:00
abstract：:Chaperone DnaK and its co-chaperone DnaJ plays various essential roles such as in assisting in the folding of nascent peptides, preventing protein aggregation and maintaining cellular protein homeostasis. Global transcriptional changes in vivo associated with deletion of dnaKJ were monitored using DNA microarray to el...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.03.001
更新日期：2016-05-01 00:00:00
abstract：:Pythium intermedium is known to play an important role in the carbon cycling of cool-temperate forest soils. In this study, a fast, precise and effective real-time PCR technique for estimating the population densities of P. intermedium from soils was developed using species-specific primers. Specificity was confirmed ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.11.010
更新日期：2010-10-20 00:00:00
abstract：:Methicillin-resistant staphylococci may also be resistant to some other antibiotics as well as beta-lactams. In this study, co-existence of resistance to methicillin and aminoglycosides was genetically investigated in staphylococci. A total of 50 staphylococci from in-patients, 17 Staphylococcus aureus and 33 coagulas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.05.002
更新日期：2006-01-01 00:00:00
abstract：:Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzy...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.012
更新日期：2007-01-01 00:00:00
abstract：:A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.011
更新日期：2015-08-01 00:00:00
abstract：:The inhibitory effect of onion oil against the growth of various isolates of bacteria representing Gram-positive (4 isolates) and Gram-negative (4 isolates) species were studied. Results show that onion oil was highly active against all Gram-positive bacteria tested and only one isolate (Klebsiella pneumoniae) of Gram...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(11)80052-2
更新日期：1995-05-01 00:00:00
abstract：:A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. Although there was ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.07.005
更新日期：2011-07-20 00:00:00
abstract：:The methylotrophic yeast, Ogataea thermomethanolica TBRC656, is an attractive host organism for heterologous protein production owing to the availability of protein expression vectors and a genome-editing tool. In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126372
更新日期：2020-02-01 00:00:00
abstract：:The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.04.017
更新日期：2016-09-01 00:00:00
abstract：:The functions of two long-chain fatty acid CoA ligase genes (facl) in crude oil-degrading Geobacillus thermodenitrificans NG80-2 were characterized. Facl1 and Facl2 encoded by GTNG_0892 and GTNG_1447 were expressed in Escherichia coli and purified as His-tagged fusion proteins. Both enzymes utilized a broad range of f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.05.001
更新日期：2012-12-20 00:00:00
