Analysis of the transcriptional regulation of YlODC gene from the dimorphic fungus Yarrowia lipolytica.

Abstract:

:Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytica (YlODC) was analyzed. To this end we made a transcriptional fusion of its promoter with the ORF of the beta-glucuronidase gene from Escherichia coli, and analyzed the effect of polyamines and the dimorphic transition on the rate of transcription. The results obtained show that there exists a weak but reproducible transcriptional regulation of YlODC under the analyzed conditions. Our data suggest that gene transcription is down-regulated by putrescine, and stimulated by spermidine. It is suggested that the simultaneous operation of transcriptional and post-translational regulatory mechanisms endows the cell with a more efficient mechanism to control the physiological processes that depend on polyamines.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Jiménez-Bremont JF,Ruiz-Herrera J

doi

10.1016/j.micres.2008.05.001

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

717-23

issue

6

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(08)00028-1

journal_volume

163

pub_type

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