Abstract:
:Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results can identify these sRNAs in a genome-wide detection, using RNA sequencing (RNA-seq) by the deep sequencing technique. In this study, the raw data of radiation-exposed samples (RS) and control samples (CS) were acquired separately from the sequencing platform. There were 217 common sRNA candidates in the two samples screened in the genome-wide scale by bioinformatics analysis. There were 43 differentially expressed sRNA candidates, including 28 up-regulated and 15 down-regulated ones. The down-regulated sRNAs were selected for the sRNA target prediction, of which 12 sRNAs that may modulate the genes related to the transcription regulation and DNA repair were considered as the candidates involved in the radio-resistance regulation system.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Chen Z,Li L,Shan Z,Huang H,Chen H,Ding X,Guo J,Liu Ldoi
10.1016/j.micres.2016.06.001subject
Has Abstractpub_date
2016-11-01 00:00:00pages
122-129eissn
0944-5013issn
1618-0623pii
S0944-5013(16)30297-Xjournal_volume
192pub_type
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