Abstract：
:Extracellular proteases from 8 Antarctic psychrotolerant Pseudomonas sp. strains were purified and characterised. All of them are neutral metalloproteases, have an apparent molecular mass of 45kDa, optimal activity at 40 degrees C and pH 7-9, retaining significant activity at pH 5-11. With the exception of P96-18, which is less stable, all retain more than 50% activity after 3 h of incubation at pH 5-9 and show low thermal stability (their half-life times range from 20 to 60 min at 40 degrees C and less than 5 min at 50 degrees C). These proteases can be used in commercial processes carried out at neutral pH and moderate temperatures, and are of special interest for their application in mixtures of enzymes where final thermal selective inactivation is needed. Results also highlight the relevance of Antarctic biotopes for the isolation of protease-producing enzymes active at low temperatures.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Vazquez SC,Coria SH,MacCormack WPdoi
10.1016/j.micres.2004.03.001subject
Has Abstractpub_date
2004-01-01 00:00:00pages
157-66issue
2eissn
0944-5013issn
1618-0623journal_volume
159pub_type
杂志文章abstract：:The enolase EnoA1 of Lactobacillus plantarum is here shown to interact with human plasminogen (Plg). By sequence alignment of EnoA1 with Streptococcus pneumoniae and Bifidobacterium lactis enolases, we identified BS1 and BS2 Plg-binding sites. A structure prediction of EnoA1 showed lysine residues in position 255 (BS2...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.10.001
更新日期：2013-02-22 00:00:00
abstract：:By chemical permeabilization of E. coli cells with detergents and membrane active peptides considerable amounts of the periplasmic anti-PC-miniantibodies can be released. Releases of active miniantibodies of 31%, respectively of 38%, were obtained by exposing low cell density suspensions to nonionic detergents like Tr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80056-0
更新日期：1997-12-01 00:00:00
abstract：:Changes in microbial numbers and activities in a soil in response to bentazon applied at 10 and 100 ppm were studied after 4 and 30 weeks of incubation in laboratory conditions. As regards the eight general and functional microbial groups studied (aerobic and anaerobic bacteria, fungi, aerobic and anaerobic N2-fixing ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80064-4
更新日期：1996-03-01 00:00:00
abstract：:Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that convert cytidine (C) to thymine (T) without DSBs have been developed extensively in m...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.03.007
更新日期：2019-01-01 00:00:00
abstract：:Extended spectrum beta-lactamases (ESBLs) usually associated with multiple drug resistance, including beta-lactam and non-beta-lactam antibiotics. This resistance can cause Limitation in the choice of drugs appropriate for using in clinical practice, especially in life-threatening infections. In this study we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.10.001
更新日期：2005-01-01 00:00:00
abstract：:Deciphering host innate immune function and bacterial pathogenic tactics require a system that facilitates both facets of host-pathogen interactions. In recent years, a model that becomes established in dissecting mechanisms of host antibacterial immune response through probing with a potent bacterial pathogen involve...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2020.126568
更新日期：2020-11-01 00:00:00
abstract：:Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.08.003
更新日期：2017-12-01 00:00:00
abstract：:In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella mel...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.012
更新日期：2008-01-01 00:00:00
abstract：:For a long a time programmed cell death was thought to be a unique characteristic of higher eukaryotes, but evidence has accumulated showing that programmed cell death is a universal phenomenon in all life forms. Many different types of bacterial programmed cell death systems have been identified, rivalling the eukary...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.11.016
更新日期：2018-03-01 00:00:00
abstract：:Metal tolerance has been found to vary among Acidithiobacillus ferrooxidans strains and this can impact the efficiency of biomining practices. To explain observed strain variability for differences in metal tolerance we examined the effects of Cu(2+) and Ni(2+) concentrations (1-200 mM) on cytoplasmic membrane propert...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.03.004
更新日期：2011-03-20 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:Mycobacterium tuberculosis Rv2140c is a function unknown conserved phosphatidylethanolamine-binding protein (PEBP), homologous to Raf kinase inhibitor protein (RKIP) in human beings. To delineate its function, we heterologously expressed Rv2140c in a non-pathogenic M. smegmatis. Quantitative phosphoproteomic analysis ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126615
更新日期：2021-01-01 00:00:00
abstract：:The genus Enterococcus belong to the genera of bacteria that produce lactic acid and can confer health benefits to living organisms. Selenium (Se) is an essential micronutrient for humans and animals. Thirty-six Enterococcus species isolated from dairy products were screened for Se(IV) sorption capacity for use as a p...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.03.005
更新日期：2011-03-20 00:00:00
abstract：:Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.04.004
更新日期：2009-01-01 00:00:00
abstract：:A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.07.007
更新日期：2006-01-01 00:00:00
abstract：:Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.07.009
更新日期：2018-10-01 00:00:00
abstract：:Yeast colonies isolated from vineyard and cellar substrates were analysed in the present study. Yeast species assessment was carried out by amplification and digestion of a region of the ribosomal RNA gene repeat unit. Saccharomyces strains were also characterised using mitochondrial DNA restriction analysis. Oxidativ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00163
更新日期：2002-01-01 00:00:00
abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.007
更新日期：2010-02-28 00:00:00
abstract：:The complete genomic DNA sequence was determined for one of the gene for the elongation factor 1 alpha (TEF), isolated from the zygomycete Absidia glauca. Sequence comparison with TEF genes from other fungi show the highest similarity to TEF-genes of the closely related zygomycete Mucor racemosus (Sundstrom et al. 198...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(11)80035-2
更新日期：1995-03-01 00:00:00
abstract：:We have now entered in the post-genomic era, where we have knowledge of plethora of fungal genomes and cutting edge technology is available to study global mRNA, protein and metabolite profiles. These so-called 'omic' technologies (transcriptomics, proteomics and metabolomics) provide the possibility to characterize p...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2007.06.006
更新日期：2007-01-01 00:00:00
abstract：:The ATP-dependent Lon protease is universally distributed in bacteria, eukaryotic organelles and archaea. In comparison with bacterial and eukaryal Lon proteases, the biology of the archaeal Lon has been studied to a limited extent. In this study, the gene encoding the Lon protease of the alkaliphilic haloarchaeon Nat...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.07.002
更新日期：2011-05-20 00:00:00
abstract：:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.006
更新日期：2007-01-01 00:00:00
abstract：:Non-conventional yeasts are increasingly applied in fermented beverage industry to obtain distinctive products with improved quality. Among these yeasts, Lachancea thermotolerans has multiple features of industrial relevance, especially the production of l(+)-lactic acid (LA), useful for the biological acidification o...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126525
更新日期：2020-09-01 00:00:00
abstract：:In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were abl...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80002-6
更新日期：2000-03-01 00:00:00
abstract：:Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126685
更新日期：2021-01-05 00:00:00
abstract：:Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a sing...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.10.007
更新日期：2016-01-01 00:00:00
abstract：:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.005
更新日期：2007-01-01 00:00:00
abstract：:Monodon baculovirls (MBV), external fouling organisms (EFO) and bacteria (especially Vibrio species) were monitored during 1996-1997 at nine different Penaeus monodon rearing hatcheries in India. Total cultivable heterotrophic bacteria, Vibrio-like-bacteria, presumptive Vibrio harveyi, Vibrio anguillarum, Vibrio vulni...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00208
更新日期：2003-01-01 00:00:00
abstract：:Flavin adenine dinucleotide (FAD)-binding proteins play a vital role in energy transfer and utilization during fungal growth and mycelia aggregation. We sequenced the genome of Volvariella volvacea, an economically important edible fungus, and discovered 41 genes encoding FAD-binding proteins. Gene expression profiles...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.02.009
更新日期：2013-10-01 00:00:00
abstract：:Xanthomonas axonopodis pv. punicae (Xap), the bacterial blight pathogen of pomegranate, incurs substantial loss to yield and reduces export quality of this economically important fruit crop. During infection, the bacterium secretes six non-TAL (Xop) effectors into the pomegranate cells through a specialized type three...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126560
更新日期：2020-11-01 00:00:00
