Abstract:
:By chemical permeabilization of E. coli cells with detergents and membrane active peptides considerable amounts of the periplasmic anti-PC-miniantibodies can be released. Releases of active miniantibodies of 31%, respectively of 38%, were obtained by exposing low cell density suspensions to nonionic detergents like Triton X-100 and tetraethyleneglycolmonodecylether. At high cell densities releasing levels of 40% and 56% were observed for these detergents. The addition of cationic, membrane active peptides (PMBN, polylysine 115, magainin II and melittin) led to release of up to 20% of active miniantibodies at low cell densities. The excretion of miniantibodies at low cell densities was increased up to 35% by phospholipase A2. Interestingly, the membrane associating properties of the anti-PC-miniantibodies influenced the permeability of the outer membrane and the excretion of beta-lactamase.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Mörbe JL,Riesenberg Ddoi
10.1016/S0944-5013(97)80056-0subject
Has Abstractpub_date
1997-12-01 00:00:00pages
385-94issue
4eissn
0944-5013issn
1618-0623pii
S0944-5013(97)80056-0journal_volume
152pub_type
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