Release of miniantibodies from E. coli cells into the supernatant at low and high cell densities.

Abstract:

:By chemical permeabilization of E. coli cells with detergents and membrane active peptides considerable amounts of the periplasmic anti-PC-miniantibodies can be released. Releases of active miniantibodies of 31%, respectively of 38%, were obtained by exposing low cell density suspensions to nonionic detergents like Triton X-100 and tetraethyleneglycolmonodecylether. At high cell densities releasing levels of 40% and 56% were observed for these detergents. The addition of cationic, membrane active peptides (PMBN, polylysine 115, magainin II and melittin) led to release of up to 20% of active miniantibodies at low cell densities. The excretion of miniantibodies at low cell densities was increased up to 35% by phospholipase A2. Interestingly, the membrane associating properties of the anti-PC-miniantibodies influenced the permeability of the outer membrane and the excretion of beta-lactamase.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Mörbe JL,Riesenberg D

doi

10.1016/S0944-5013(97)80056-0

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

385-94

issue

4

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(97)80056-0

journal_volume

152

pub_type

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