Abstract：
:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by transferring resistance determinants via conjugation and transformation, and by detecting bla genes with PCR analysis. The six strains showed two different genomic patterns on pulsed-field gel electrophoresis. All the transconjugants and transformants gained high-level resistance to ampicillin, cephalexin, cefoxitin and cefotaxime, and showed a reduced susceptibility to imipenem, but maintained full susceptibility to aztreonam. In addition, the expressions of blaIMP and blaTEM genes were constitutive, either in Serratia marcescens clinical isolates or in their transconjugants and transformants. These findings may explain the rapid spread of the above resistance determinants among Enterobacteriaceae via transmissible plasmids in the clinical setting.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Zhao WH,Hu ZQ,Chen G,Matsushita K,Fukuchi K,Shimamura Tdoi
10.1016/j.micres.2006.06.005subject
Has Abstractpub_date
2007-01-01 00:00:00pages
46-52issue
1eissn
0944-5013issn
1618-0623pii
S0944-5013(06)00066-8journal_volume
162pub_type
杂志文章abstract：:DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interact...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126650
更新日期：2021-02-01 00:00:00
abstract：:A mercury-resistant plasmid of pTMJ212 which was able to shuttle between Acidithiobacillus caldus and Escherichia coli was constructed by inserting the mercury resistant determinants, the mer operon of Acidithiobacillus ferrooxidans, into the IncQ plasmid of pJRD215. pTMJ212 was transferred from Escherichia coli into ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.10.003
更新日期：2011-10-20 00:00:00
abstract：:The coral-killing invasive sponge, Terpios hoshinota, causes extensive mortality to live corals and is a potential threat to reefs at different geographical locations. However, to date, the invasive mechanism remains largely unknown. In this study, we aimed to understand the bacterial competition between sponge and co...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126553
更新日期：2020-11-01 00:00:00
abstract：:Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characte...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.008
更新日期：2018-11-01 00:00:00
abstract：:Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular size...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.010
更新日期：2005-01-01 00:00:00
abstract：:A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00127
更新日期：2002-01-01 00:00:00
abstract：:Saccharomyces cerevisiae and Candida shehatae were co-immobilized in an agar sheet which was introduced in an original two-chambered bioreactor asymmetrically fed in a batch mode with a mixture of glucose and xylose in a ratio of 35:15. The two sugars were consumed simultaneously. All glucose was fermented but only 20...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.005
更新日期：2007-01-01 00:00:00
abstract：:Outer membrane protein U (OmpU) is a major porin from Vibrio alginolyticus and has been considered a vaccine candidate against infection by V. alginolyticus. After pre-incubated with polyclonal antibody against rOmpU, V. alginolyticus showed a 78% decrease in extracellular iron level, suggesting that interruption of O...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126350
更新日期：2020-01-01 00:00:00
abstract：:In this study, the biosurfactant production by an Aureobasidium thailandense LB01 was reported for the first time. Different agro-industrial by-products (corn steep liquor, sugarcane molasses, and olive oil mill wastewater) were evaluated as alternative low-cost substrates. The composition of the culture medium was op...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.07.004
更新日期：2017-11-01 00:00:00
abstract：:The use of biocontrol strains recently has become a popular alternative to conventional chemical treatments. A set of bacteria isolated from sugar beet rhizosphere and from roots and shoots of apple and walnut were evaluated for their potential to control sugar beet seedling damping-off caused by R. solani AG-4 and AG...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.06.011
更新日期：2016-11-01 00:00:00
abstract：:Three classes of cell wall-defective mutants of the unicellular green alga Chlamydomonas reinhardtii have been described in the literature differing with respect to the amounts of cell wall material and its attachment to the plasma membrane, respectively. We have compared the production of the chaotrope-soluble cell w...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80012-2
更新日期：1997-07-01 00:00:00
abstract：:Annual ryegrass is a fast-growing cool-season grass broadly present in the Portuguese "montado", a typically Mediterranean agro-forestry-pastoral ecosystem. A culture-dependent approach was used to investigate natural associations of this crop with potentially beneficial bacteria, aiming to identify strains suitable f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.12.010
更新日期：2014-09-01 00:00:00
abstract：:Deciphering host innate immune function and bacterial pathogenic tactics require a system that facilitates both facets of host-pathogen interactions. In recent years, a model that becomes established in dissecting mechanisms of host antibacterial immune response through probing with a potent bacterial pathogen involve...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2020.126568
更新日期：2020-11-01 00:00:00
abstract：:Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electropho...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80026-7
更新日期：1996-08-01 00:00:00
abstract：:Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.07.001
更新日期：2010-01-01 00:00:00
abstract：:Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.12.006
更新日期：2019-03-01 00:00:00
abstract：:We previously reported that inactivation of a universally conserved dimethyl adenosine transferase (KsgA) attenuates virulence and increases sensitivity to oxidative and osmotic stress in Salmonella Enteritidis. Here, we show a role of KsgA in cell-envelope fitness as a potential mechanism underlying these phenotypes ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.009
更新日期：2018-11-01 00:00:00
abstract：:The community and Cd-resistance of endophytic fungi from roots of Salix variegata Franch. collected from the water-level-fluctuation zone of Three Gorges Reservoir Region, China, were investigated. A total of 53 strains were isolated and identified to 13 morphotaxa, in which Chromosporium, Fusarium and Gonatobotrys we...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.03.013
更新日期：2015-07-01 00:00:00
abstract：:Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.11.005
更新日期：2018-03-01 00:00:00
abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.007
更新日期：2010-02-28 00:00:00
abstract：:The genes that encode the alpha and beta subunits of protocatechuate 3,4-dioxygenase (3,4-PCD [EC 1.13.11.3]) were cloned from a Pseudomonas marginata genomic library. These genes pcaG and pcaH, were found when screening the library for hydrolase genes. The two open reading frames of the PCD genes could be identified ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(96)80004-8
更新日期：1996-12-01 00:00:00
abstract：:Although the use of crop-associated bacteria as biological control agents of fungal diseases has gained increasing interest, the biotechnological potential of forest tree-associated microbes and their natural products has scarcely been investigated. The objective of this study was to identify bacteria or bacterial pro...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126440
更新日期：2020-05-01 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:The greater wax moth Galleria mellonella has been increasingly used as a model host to determine Candida albicans virulence and efficacy of antifungal treatment. The G. mellonella lysozyme, similarly to its human counterpart, is a member of the c-type family of lysozymes that exhibits antibacterial and antifungal acti...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.10.003
更新日期：2016-12-01 00:00:00
abstract：:Alternaria species have been recognized as important cause of allergic diseases and are considered opportunistic pathogens for immunocompromised individuals. In order to assess the contribution of waters to the spread of Alternaria, we examined 390 water samples of various origin for the presence of these microorganis...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80022-1
更新日期：2000-04-01 00:00:00
abstract：:Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The ex...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.08.004
更新日期：2015-01-01 00:00:00
abstract：:The complete genomic DNA sequence was determined for one of the gene for the elongation factor 1 alpha (TEF), isolated from the zygomycete Absidia glauca. Sequence comparison with TEF genes from other fungi show the highest similarity to TEF-genes of the closely related zygomycete Mucor racemosus (Sundstrom et al. 198...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(11)80035-2
更新日期：1995-03-01 00:00:00
abstract：:Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.06.009
更新日期：2018-10-01 00:00:00
abstract：:We have cloned a laccase gene fragment isolated from a Trametes versicolor strain in Korea. It showed high similarity in nucleotide sequences when compared with other fungal laccases. TNT (2,4,6-trinitrotoluene), a widely used explosive, was transformed rapidly by T. versicolor. When TNT and its catabolic intermediate...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.12.001
更新日期：2006-01-01 00:00:00
