Abstract：
:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separately at 1.0%, 1.5%, 2.0% and 2.5% levels to the basal medium in order to assess their potential on the growth and concentration of pigments, viz. Chl a, Chl b and beta-carotene of the alga. beta-Carotene was isolated and visible absorption spectrum was taken at 443 and 475 nm confirmed the presence of 9-cis-beta-carotene and all-trans-beta-carotene isomers. Maximum yield, highest division rate (mu) and highest pigment concentrations were observed in the cells grown in 1.5% S. wightii and 2.0% U. lactuca amended medium and these cells were subjected to DAPI staining. The results of epifluorescence microscopy and image analysis revealed a significant enhancement of the cell and nuclear area of the microalgae.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Raja R,Hema Iswarya S,Balasubramanyam D,Rengasamy Rdoi
10.1016/j.micres.2006.03.006subject
Has Abstractpub_date
2007-01-01 00:00:00pages
168-76issue
2eissn
0944-5013issn
1618-0623pii
S0944-5013(06)00030-9journal_volume
162pub_type
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