Abstract：
:Ammonia production from urea by ureolytic oral bacteria is believed to have a significant impact on oral health and ecological balance of oral microbial populations. Actinomyces naeslundii is an important ureolytic organism in the oral cavity. In this study, we aimed to investigate the substrate affinity and pH optimum for ureolysis of A. naeslundii (ATCC12104), and expression of urease under different environmental factors. In addition, in vitro acid killing and pH drop experiments were used to detect the role of ureolysis in bacterial aciduricity and capacity to modulate pH homeostasis. We observed the K(s) value of the ureolytic activity was 7.5mM and a pH optimum near 6.5. Urease expression by A. naeslundii (ATCC12104) was affected by multiple factors, including environmental pH, glucose and nitrogen availability. The cells could be protected against acid killing through hydrolysis of physiologically relevant concentrations of urea. A. naeslundii (ATCC12104) demonstrated a significant capacity to temper glycolytic acidification in vitro at urea concentrations normally found in the oral cavity.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Liu Y,Hu T,Zhang J,Zhou Xdoi
10.1016/j.micres.2005.11.002subject
Has Abstractpub_date
2006-01-01 00:00:00pages
304-10issue
4eissn
0944-5013issn
1618-0623pii
S0944-5013(05)00119-9journal_volume
161pub_type
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