Abstract：
:Bacteria of the genus Agrobacterium can transfer a portion of their Ti plasmid (T-DNA) in complex with the VirE2 and VirD2 proteins into the plant-cell nucleus and cause it to be integrated in the host-cell chromosomes. The mechanism of T-DNA transfer across the plant-cell membrane and cytoplasm is unknown. The aim of this study was to isolate the virulence protein VirE2 in order to explore its role in T-DNA transfer across the eukaryotic-cell membrane and cytoplasm. To obtain VirE2, we cloned the virE2 gene into plasmid pQE31 in Escherichia coli cells. VirE2 protein was isolated from E. coli XL-1 blue cells containing a recombinant plasmid, pQE31-virE2. The cells were ultrasonically disrupted, and the protein containing six histidine residues at the N-terminal end was isolated by affinity chromatography on Ni-NTA agarose. The purified preparation was tested by immunodot, by using polyclonal rabbit antibodies and miniantibodies produced toward VirE2. The capacity of the recombinant protein VirE2 for interacting with single-stranded DNA was tested by the formation of complexes, recorded by agarose-gel electrophoresis. In summary, A. tumefaciens virulence protein VirE2, capable of forming a complex with single-stranded T-DNA during transfer into the plant cell, was isolated, purified, and partially characterized. Anti-VirE2 miniantibodies were obtained, and direct labeling of VirE2 with colloidal gold was done for the first time.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Volokhina I,Sazonova I,Velikov V,Chumakov Mdoi
10.1016/j.micres.2004.09.012subject
Has Abstractpub_date
2005-01-01 00:00:00pages
67-73issue
1eissn
0944-5013issn
1618-0623pii
S0944-5013(04)00083-7journal_volume
160pub_type
杂志文章abstract：:Plant growth promoting rhizobacteria (PGPR) are potential agents to control plant pathogens and their combined use with biopesticides such as phosphites may constitute a novel strategy to incorporate in disease management programs. In the present study, 11 bacterial isolates were selected on the basis of their antagon...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.07.004
更新日期：2015-11-01 00:00:00
abstract：:The rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. Fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. Acremonium sp....
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00138
更新日期：2002-01-01 00:00:00
abstract：:Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.06.009
更新日期：2018-10-01 00:00:00
abstract：:Desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. We demonstrated the existence of Δ9 desaturase activity in epimastigotes of the Try...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.07.012
更新日期：2017-01-01 00:00:00
abstract：:Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.04.004
更新日期：2009-01-01 00:00:00
abstract：:Metal tolerance has been found to vary among Acidithiobacillus ferrooxidans strains and this can impact the efficiency of biomining practices. To explain observed strain variability for differences in metal tolerance we examined the effects of Cu(2+) and Ni(2+) concentrations (1-200 mM) on cytoplasmic membrane propert...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.03.004
更新日期：2011-03-20 00:00:00
abstract：:Flavin adenine dinucleotide (FAD)-binding proteins play a vital role in energy transfer and utilization during fungal growth and mycelia aggregation. We sequenced the genome of Volvariella volvacea, an economically important edible fungus, and discovered 41 genes encoding FAD-binding proteins. Gene expression profiles...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.02.009
更新日期：2013-10-01 00:00:00
abstract：:Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.001
更新日期：2005-01-01 00:00:00
abstract：:Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.07.001
更新日期：2010-01-01 00:00:00
abstract：:Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electropho...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80026-7
更新日期：1996-08-01 00:00:00
abstract：:Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial di...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.06.005
更新日期：2009-01-01 00:00:00
abstract：:The genes that encode the alpha and beta subunits of protocatechuate 3,4-dioxygenase (3,4-PCD [EC 1.13.11.3]) were cloned from a Pseudomonas marginata genomic library. These genes pcaG and pcaH, were found when screening the library for hydrolase genes. The two open reading frames of the PCD genes could be identified ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(96)80004-8
更新日期：1996-12-01 00:00:00
abstract：:The orphan histidine kinase (HK) from Streptomyces peucetius ATCC 27952 (ohkAsp) was found to be implicated in the regulation of doxorubicin (DOX)/daunorubicin (DNR) biosynthesis, self-defense and developmental attributes. OhkAsp is a homolog of OhkA from Streptomyces coelicolor and Streptomyces avermitilis (with 73 a...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.05.007
更新日期：2018-09-01 00:00:00
abstract：:Foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. Nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxon...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.06.002
更新日期：2008-01-01 00:00:00
abstract：:Bitespiramycin (biotechnological spiramycin, Bsm) is a new 16-membered macrolide antibiotic produced by Streptomyces spiramyceticus WSJ-1 integrated exogenous genes. The gene cluster for Bsm biosynthesis consists of two parts: spiramycin biosynthetic gene cluster (92 kb) and two exogenous genes including 4"-O-isovaler...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126532
更新日期：2020-11-01 00:00:00
abstract：:Araucaria angustifolia, a unique species of this genus that occurs naturally in Brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. Root-associated bacteria from A. angustifolia were...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.03.003
更新日期：2012-01-20 00:00:00
abstract：:Although it is known that oxalic acid provides a selective advantage to the secreting microbe our understanding of how this acid is biosynthesized remains incomplete. This study reports the identification, cloning, and partial characterization of the oxalic acid biosynthetic enzyme from the animal bacterial pathogen, ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.11.002
更新日期：2011-10-20 00:00:00
abstract：:The present investigation reveals the solubilization efficiency of tri-calcium phosphate (TCP), Udaipur rock phosphate (URP), aluminium phosphate (AP) and ferric phosphate (FP) by Aspergillus niger (ITCC 6719) and Trichoderma harzianum (ITCC 6721) as function of carbon concentrations. Increasing glucose concentration ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.09.005
更新日期：2016-12-01 00:00:00
abstract：:The complete genomic DNA sequence was determined for one of the gene for the elongation factor 1 alpha (TEF), isolated from the zygomycete Absidia glauca. Sequence comparison with TEF genes from other fungi show the highest similarity to TEF-genes of the closely related zygomycete Mucor racemosus (Sundstrom et al. 198...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(11)80035-2
更新日期：1995-03-01 00:00:00
abstract：:Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The ex...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.08.004
更新日期：2015-01-01 00:00:00
abstract：:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.005
更新日期：2007-01-01 00:00:00
abstract：:Hirsutella rhossiliensis OWVT-1 has substantial potential as a biocontrol agent against plant-parasitic nematodes. Serine proteases have emerged as a potentially useful factor in the nematode-fungus interactions. When grown in liquid culture with the nematode Panagrellus redivivus as the sole nitrogen source, an extra...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.01.003
更新日期：2009-01-01 00:00:00
abstract：:Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126650
更新日期：2021-02-01 00:00:00
abstract：:In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to t...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80035-X
更新日期：2000-09-01 00:00:00
abstract：:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.01.001
更新日期：2004-01-01 00:00:00
abstract：:There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.01.009
更新日期：2007-01-01 00:00:00
abstract：:One strain of Lactobacillus acidophilus was found to produce a bacteriocin-like substance in the culture filtrate. The substance was produced in a growth-associated manner, showed heat stability at neutral and acidic pH and exhibited antibacterial activity against various species of Lactobacillus including L. acidophi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00190
更新日期：2003-01-01 00:00:00
abstract：:Evaluation of plant growth promoting bacteria and the associated metabolites under saline conditions can be a potential eco-friendly remediation and productivity enhancement strategy. Salt-tolerant Pseudomonas entomophila PE3 was isolated from saline soil and screened for plant growth promoting (PGP) traits. The isola...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126671
更新日期：2021-03-01 00:00:00
abstract：:In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella mel...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.012
更新日期：2008-01-01 00:00:00
abstract：:Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.01.005
更新日期：2015-02-01 00:00:00
