Abstract：
:Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% of studied strains there was a concordance between slime production and presence of icaA and icaD genes, and between lack of slime production and absence of both or only one of the tested genes. In four of five strains where positive phenotype was not associated with the presence of ica genes, gene co-expression (evaluated by mRNA determination) was lacking, while in only a case out of five, there was the presence of transcripts without phenotype. Our study, for the first time, introduces real-time PCR for the detection of both icaA and icaD genes and their mRNA and, furthermore, addresses the relationship between slime phenotype absence and mRNA expression, in clinical isolates of coagulase-negative staphylococci.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Liberto MC,Matera G,Quirino A,Lamberti AG,Capicotto R,Puccio R,Barreca GS,Focà E,Cascio A,Focà Adoi
10.1016/j.micres.2007.04.004subject
Has Abstractpub_date
2009-01-01 00:00:00pages
522-8issue
5eissn
0944-5013issn
1618-0623pii
S0944-5013(07)00063-8journal_volume
164pub_type
杂志文章abstract：:Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense an...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.11.012
更新日期：2016-02-01 00:00:00
abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.005
更新日期：2010-02-28 00:00:00
abstract：:In this study, we evaluated the efficiency of six isolates of Saccharomyces cerevisiae in controlling Colletotrichum acutatum, the causal agent of postbloom fruit drop that occur in pre-harvest citrus. We analyzed the mechanisms of action involved in biological control such as: production of antifungal compounds, nutr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.003
更新日期：2015-06-01 00:00:00
abstract：:The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an overse...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00067
更新日期：2001-01-01 00:00:00
abstract：:Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5-8.7) which are mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline soils ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.07.007
更新日期：2014-01-20 00:00:00
abstract：:The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi. Culture supernatants of 17 Lactic acid bacterial strains as well as of three commercial probiotic cultures were evaluated for antifungal activity using an agar-diffusion method. The me...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.07.002
更新日期：2004-01-01 00:00:00
abstract：:The conditions for the sequential production of antibiotic activity by Talaromyces flavus were determined. The highest level of activity against Trypanosoma cruzi was obtained from the aqueous extract of the Czapeck's fermentative culture after 48 hours, with lysis of 97.58% of the trypomastigote forms of Trypanosoma ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00148
更新日期：2002-01-01 00:00:00
abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.007
更新日期：2010-02-28 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.06.008
更新日期：2015-10-01 00:00:00
abstract：:Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been subjected to intensive research due to their biotechnological potential as crop inoculants. Phylogenetic analysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsistencies and low ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.11.009
更新日期：2017-01-01 00:00:00
abstract：:Bacterial carbonate precipitation has implications in geological processes and important biotechnological applications. Bacteria capable of precipitating carbonates have been isolated from different calcium carbonate deposits (speleothems) in caves, soil, freshwater and seawater around the world. However, the diversit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.09.006
更新日期：2016-01-01 00:00:00
abstract：:Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.001
更新日期：2005-01-01 00:00:00
abstract：:Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial di...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.06.005
更新日期：2009-01-01 00:00:00
abstract：:Seeds are reservoirs of beneficial and harmful microorganism that modulates plant growth and health. Here, we access seed to seedling bacteriome assembly modified by seed-disinfection and the underlined effect over maize germination performance and root-seedlings microbial colonization. Seed-disinfection was performed...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126643
更新日期：2021-02-01 00:00:00
abstract：:Signal recognition particle (SRP) mediates targeting of proteins to appropriate cellular compartments, which is an important process in all living organisms. In prokaryotes, SRP consists of Ffh, a protein, and 4.5S RNA that recognizes signal peptide emerging from ribosomes. The SRP (Ffh) of one the most successful int...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.03.002
更新日期：2012-10-12 00:00:00
abstract：:Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.011
更新日期：2013-11-07 00:00:00
abstract：:A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.07.007
更新日期：2006-01-01 00:00:00
abstract：:Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.08.003
更新日期：2017-12-01 00:00:00
abstract：:The potassium solubilizing microorganisms (KSMs) are a rhizospheric microorganism which solubilizes the insoluble potassium (K) to soluble forms of K for plant growth and yield. K-solubilization is carried out by a large number of saprophytic bacteria (Bacillus mucilaginosus, Bacillus edaphicus, Bacillus circulans, Ac...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2013.09.003
更新日期：2014-05-01 00:00:00
abstract：:The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.010
更新日期：2008-01-01 00:00:00
abstract：:Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.06.001
更新日期：2016-11-01 00:00:00
abstract：:Vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V. vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.002
更新日期：2009-01-01 00:00:00
abstract：:Saccharomyces cerevisiae and Candida shehatae were co-immobilized in an agar sheet which was introduced in an original two-chambered bioreactor asymmetrically fed in a batch mode with a mixture of glucose and xylose in a ratio of 35:15. The two sugars were consumed simultaneously. All glucose was fermented but only 20...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.005
更新日期：2007-01-01 00:00:00
abstract：:The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.08.016
更新日期：2018-01-01 00:00:00
abstract：:Annual ryegrass is a fast-growing cool-season grass broadly present in the Portuguese "montado", a typically Mediterranean agro-forestry-pastoral ecosystem. A culture-dependent approach was used to investigate natural associations of this crop with potentially beneficial bacteria, aiming to identify strains suitable f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.12.010
更新日期：2014-09-01 00:00:00
abstract：:Investigating the entire species composition of the microorganisms is crucial to understand their roles in the biogeochemical cycles. Metagenomic DNA was extracted from six intertidal sediment samples along the Liaodong Bay of Bohai Sea, China, and was sequenced which yielded a total of 64,496 high-quality sequences f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.09.019
更新日期：2014-07-01 00:00:00
abstract：:Leukotoxins produced by staphylococci, especially Staphylococcus aureus, have long been considered important virulence determinants. The present assay examined 26 strains of staphylococci isolated from sheep for their ability to produce factors leukotoxic to polymorphonuclear leukocytes deriving from ovine mammary gla...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80035-3
更新日期：1997-09-01 00:00:00
abstract：:Western immunoblot analyses of whole cell lysates probed with a human specific monoclonal anti-topoisomerase IIalpha antibody identified a 190 kDa protein over expressed in the arsenite resistant Leishmania donovani strain. The crude nuclear extract of the resistant strain showed higher topoisomerase II-like enzyme ac...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00177
更新日期：2003-01-01 00:00:00
abstract：:Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.001
更新日期：2017-07-01 00:00:00
abstract：:There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.01.009
更新日期：2007-01-01 00:00:00
