Abstract：
:A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Fernández F,Gutiérrez J,Sorlózano A,Romero JM,Soto MJ,Ruiz-Cabello Fdoi
10.1016/j.micres.2005.07.007subject
Has Abstractpub_date
2006-01-01 00:00:00pages
158-63issue
2eissn
0944-5013issn
1618-0623pii
S0944-5013(05)00077-7journal_volume
161pub_type
杂志文章abstract：:We have now entered in the post-genomic era, where we have knowledge of plethora of fungal genomes and cutting edge technology is available to study global mRNA, protein and metabolite profiles. These so-called 'omic' technologies (transcriptomics, proteomics and metabolomics) provide the possibility to characterize p...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2007.06.006
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abstract：:Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a sing...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.10.007
更新日期：2016-01-01 00:00:00
abstract：:Geopolitical and economic factors have motivated the scientific community to utilize renewable energy resources. In addition to the modifications in major steps and processes of biofuel production, manipulation of microbial genome engineering tools is essential in order to find sustainable solution of continuous deple...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2018.10.010
更新日期：2019-02-01 00:00:00
abstract：:Plant growth-promoting rhizobacteria promote plant growth by direct and indirect mechanisms. We isolated twelve bacterial strains showing different degrees of phosphate solubilizing activity from maize rhizosphere. Four isolates solubilized over 300 μg mL⁻¹ phosphate from insoluble Ca₃(PO₄)₂, with isolate SCAUK0330 so...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.07.003
更新日期：2014-01-20 00:00:00
abstract：:The greater wax moth Galleria mellonella has been increasingly used as a model host to determine Candida albicans virulence and efficacy of antifungal treatment. The G. mellonella lysozyme, similarly to its human counterpart, is a member of the c-type family of lysozymes that exhibits antibacterial and antifungal acti...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.10.003
更新日期：2016-12-01 00:00:00
abstract：:A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title：Microbiological research
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
abstract：:The inhibitory effect of onion oil against the growth of various isolates of bacteria representing Gram-positive (4 isolates) and Gram-negative (4 isolates) species were studied. Results show that onion oil was highly active against all Gram-positive bacteria tested and only one isolate (Klebsiella pneumoniae) of Gram...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(11)80052-2
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80026-7
更新日期：1996-08-01 00:00:00
abstract：:The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.06.008
更新日期：2015-10-01 00:00:00
abstract：:Vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V. vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.002
更新日期：2009-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.05.001
更新日期：2019-09-01 00:00:00
abstract：:Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene seq...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126522
更新日期：2020-10-01 00:00:00
abstract：:Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enh...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126640
更新日期：2021-01-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.09.008
更新日期：2018-01-01 00:00:00
abstract：:Cryoconite holes (cylindrical melt-holes on the glacier surface) are important hydrological and biological systems within glacial environments that support diverse microbial communities and biogeochemical processes. This study describes retrievable heterotrophic microbes in cryoconite hole water from three geographica...
journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.11.012
更新日期：2016-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.micres.2012.11.004
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journal_title：Microbiological research
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更新日期：2008-01-01 00:00:00
abstract：:Moderately halophilic bacteria are a kind of extreme environment microorganism that can tolerate moderate salt concentrations ranging from 0.5M to 2.5M. Here, via a metagenomic library screen, we identified four putative Na(+) transporters, designated H7-Nha, H16-Mppe, H19-Cap and H35-Mrp, from moderately halophilic c...
journal_title：Microbiological research
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.11.002
更新日期：2011-10-20 00:00:00
abstract：:Actinomycetes have been isolated from three Brazilian tropical soils. The dispersion and differential centrifugation procedure revealed count values 1.5 to 5.0 times greater than those obtained by the conventional dilution plate technique for all soils and media tested. Eighteen strains, promising for biotechnological...
journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2001-03-01 00:00:00
abstract：:Two morphologically different bacteriophages were isolated from the river and soil samples from various locations of Maharashtra, India against the phytopathogen Pseudomonas sp. that was recently reported to cause a new bacterial blight of pomegranate. Both the phages belonged to the order Caudovirales representing th...
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pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
abstract：:Zymography of alcohol dehydrogenase (ADH) activity in the entomopathogenic fungus Metarhizium anisopliae grown under various conditions revealed that micro-aerobic growth was associated with increased ADH activity. The major ADH protein, AdhIp, was purified to homogeneity by affinity chromatography and has an estimate...
journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
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journal_title：Microbiological research
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更新日期：2015-06-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.11.004
更新日期：2010-09-20 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.10.001
更新日期：2013-02-22 00:00:00
abstract：:Desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. We demonstrated the existence of Δ9 desaturase activity in epimastigotes of the Try...
journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
